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Article

Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines

  • Authors:
    • Reza Alizadeh‑Navaei
    • Alireza Rafiei
    • Saeid Abedian‑Kenari
    • Hossein Asgarian‑Omran
    • Reza Valadan
    • Akbar Hedayatizadeh‑Omran
  • View Affiliations / Copyright

    Affiliations: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175‑861, Iran, Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari 48175‑861, Iran
  • Pages: 130-132
    |
    Published online on: May 20, 2016
       https://doi.org/10.3892/br.2016.684
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Abstract

Evaluating the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) may be useful for predicting the best models and achieving more accurate results in cancer research. Therefore, the aim of the present study was to analyze the LGR5 expression levels in different cell lines. Eight commonly used cell lines were assessed (COS‑7, NIH3T3, HEK293, VERO, HeLa, BHK, HepG2 and AGS). All the cell lines were cultured in RPMI‑1640 medium contain 10% fetal calf serum at 37˚C in humidified conditions with 5% CO2. According to the western blotting results, LGR5 was expressed in all cell lines. Densitometry results of LGR5 expression in the different cell lines showed that high LGR5 expression levels were apparent in BHK, AGS, VERO and NIH3T3 cell lines compared with the other cell lines. The results indicate that for the normal and cancer cell lines, BNK and AGS may be a better choice, respectively, for in vitro cancer studies.
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Copy and paste a formatted citation
Spandidos Publications style
Alizadeh‑Navaei R, Rafiei A, Abedian‑Kenari S, Asgarian‑Omran H, Valadan R and Hedayatizadeh‑Omran A: Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines. Biomed Rep 5: 130-132, 2016.
APA
Alizadeh‑Navaei, R., Rafiei, A., Abedian‑Kenari, S., Asgarian‑Omran, H., Valadan, R., & Hedayatizadeh‑Omran, A. (2016). Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines. Biomedical Reports, 5, 130-132. https://doi.org/10.3892/br.2016.684
MLA
Alizadeh‑Navaei, R., Rafiei, A., Abedian‑Kenari, S., Asgarian‑Omran, H., Valadan, R., Hedayatizadeh‑Omran, A."Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines". Biomedical Reports 5.1 (2016): 130-132.
Chicago
Alizadeh‑Navaei, R., Rafiei, A., Abedian‑Kenari, S., Asgarian‑Omran, H., Valadan, R., Hedayatizadeh‑Omran, A."Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines". Biomedical Reports 5, no. 1 (2016): 130-132. https://doi.org/10.3892/br.2016.684
Copy and paste a formatted citation
x
Spandidos Publications style
Alizadeh‑Navaei R, Rafiei A, Abedian‑Kenari S, Asgarian‑Omran H, Valadan R and Hedayatizadeh‑Omran A: Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines. Biomed Rep 5: 130-132, 2016.
APA
Alizadeh‑Navaei, R., Rafiei, A., Abedian‑Kenari, S., Asgarian‑Omran, H., Valadan, R., & Hedayatizadeh‑Omran, A. (2016). Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines. Biomedical Reports, 5, 130-132. https://doi.org/10.3892/br.2016.684
MLA
Alizadeh‑Navaei, R., Rafiei, A., Abedian‑Kenari, S., Asgarian‑Omran, H., Valadan, R., Hedayatizadeh‑Omran, A."Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines". Biomedical Reports 5.1 (2016): 130-132.
Chicago
Alizadeh‑Navaei, R., Rafiei, A., Abedian‑Kenari, S., Asgarian‑Omran, H., Valadan, R., Hedayatizadeh‑Omran, A."Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines". Biomedical Reports 5, no. 1 (2016): 130-132. https://doi.org/10.3892/br.2016.684
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