Fast method for skeletal tissue gene expression analysis

Dalle Carbonare,Luca; Vilei,Maria  Teresa; Stranieri,Chiara; Innamorati,Giulio; Rosato,Antonio; Boldrin,Elisa; Sella,Stefania; Giannini,Sandro; Valenti,Maria  Teresa

doi:10.3892/br.2016.699

August-2016 Volume 5 Issue 2

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August-2016 Volume 5 Issue 2

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Article

Fast method for skeletal tissue gene expression analysis

Authors:
- Luca Dalle Carbonare
- Maria Teresa Vilei
- Chiara Stranieri
- Giulio Innamorati
- Antonio Rosato
- Elisa Boldrin
- Stefania Sella
- Sandro Giannini
- Maria Teresa Valenti
View Affiliations / Copyright

Affiliations: Department of Medicine, Section of Internal Medicine D, University of Verona, I-37134 Verona, Italy, Clinica Medica 1, Department of Medicine, University of Padova, I-35128 Padova, Italy, Department of Surgery Oncology and Gastroenterology, University of Padova, I-35128 Padova, Italy
Pages: 248-250
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Published online on: June 1, 2016

https://doi.org/10.3892/br.2016.699
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Abstract

Several chronic diseases have been associated with bone alteration in the last few years. Despite the wealth of information provided by the analysis of the transcriptome in affected tissues, only a limited number of studies evaluated gene expression in bone tissue due to the difficulty to obtain high quality RNA. Therefore, skeletal pathologies have been often associated to a defective maturation process that occurs during recruitment of progenitor stem cells. In order to explore the possibility of analysing the gene expression during osteogenic differentiation in skeletal tissue, a single‑step method to extract well‑preserved RNA from bone specimens was performed. A comparison between this technique and a traditional method was made by analysing the quality and yield of RNA obtained. In addition, RNAs were assayed by reverse transcription‑quantitative polymerase chain reaction to analyse the expression levels of the bone genes associated with the differentiation process in a mouse model. The present data showed that good quality RNA can be obtained from bone tissue by a simple single‑step method allowing the expression analysis of the genes encoded by skeletal tissue. In conclusion, the present study allows the possibility to easily obtain good quality RNA from bone tissue that is suitable for gene expression studies of bone diseases.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Fast method for skeletal tissue gene expression analysis

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Abstract

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