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A protocol for the culture and isolation of murine synovial fibroblasts

  • Authors:
    • Jinjun Zhao
    • Qingqing Ouyang
    • Ziyou Hu
    • Qin Huang
    • Jing Wu
    • Ran Wang
    • Min Yang
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology and Immunology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China, Research Center of Clinical Medicine, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 171-175
    |
    Published online on: June 28, 2016
       https://doi.org/10.3892/br.2016.708
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Abstract

The culture of synovial fibroblasts (SFs) is one of the most effective tools for investigating the pathology and physiology of synovial tissues and should prove useful for identifying the importance of SFs in disease as well as for the development of novel therapeutic approaches for several chronic joint diseases, such as rheumatoid arthritis. However, thus far, a detailed protocol for the primary culture and isolation of murine SFs has not been established. Therefore, the present study describes an easy and convenient method for isolating and culturing SFs from C57BL/6 mice. This protocol can be divided into 4 stages: Isolation of synovial tissues, isolation of SFs, seeding of SFs for growth in culture and purity analysis of SFs using the four cell markers, vimentin, cluster of differentiation 90.2 (CD90.2; Thy‑1.2), intracellular adhesion molecule 1 (CD54) and vascular cell adhesion molecule 1 (CD106). This method is efficient and a purified population of SFs can be obtained 10 days after the initiation of culture.
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1 

Emery P, Breedveld FC, Dougados M, Kalden JR, Schiff MH and Smolen JS: Early referral recommendation for newly diagnosed rheumatoid arthritis: Evidence based development of a clinical guide. Ann Rheum Dis. 61:290–297. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Dougados M, Devauchelle-Pensec V, Ferlet JF, Jousse-Joulin S, D'Agostino MA, Backhaus M, Bentin J, Chalès G, Chary-Valckenaere I, Conaghan P, et al: The ability of synovitis to predict structural damage in rheumatoid arthritis: a comparative study between clinical examination and ultrasound. Ann Rheum Dis. 72:665–671. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Bartok B and Firestein GS: Fibroblast-like synoviocytes: Key effector cells in rheumatoid arthritis. Immunol Rev. 233:233–255. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Neumann E, Lefèvre S, Zimmermann B, Gay S and Müller-Ladner U: Rheumatoid arthritis progression mediated by activated synovial fibroblasts. Trends Mol Med. 16:458–468. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Lefèvre S, Knedla A, Tennie C, Kampmann A, Wunrau C, Dinser R, Korb A, Schnäker EM, Tarner IH, Robbins PD, et al: Synovial fibroblasts spread rheumatoid arthritis to unaffected joints. Nat Med. 15:1414–1420. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Karouzakis E, Gay RE, Gay S and Neidhart M: Epigenetic control in rheumatoid arthritis synovial fibroblasts. Nat Rev Rheumatol. 5:266–272. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Noss EH and Brenner MB: The role and therapeutic implications of fibroblast-like synoviocytes in inflammation and cartilage erosion in rheumatoid arthritis. Immunol Rev. 223:252–270. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Perlman H and Pope RM: The synovial lining micromass system: Toward rheumatoid arthritis in a dish? Arthritis Rheum. 62:643–646. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Müller-Ladner U, Ospelt C, Gay S, Distler O and Pap T: Cells of the synovium in rheumatoid arthritis. Synovial fibroblasts. Arthritis Res Ther. 9:2232007. View Article : Google Scholar : PubMed/NCBI

10 

Miao CG, Huang C, Huang Y, Yang YY, He X, Zhang L, Lv XW, Jin Y and Li J: MeCP2 modulates the canonical Wnt pathway activation by targeting SFRP4 in rheumatoid arthritis fibroblast-like synoviocytes in rats. Cell Signal. 25:598–608. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Pillinger MH, Dinsell V, Apsel B, Tolani SN, Marjanovic N, Chan ES, Gomez P, Clancy R, Chang LF and Abramson SB: Regulation of metalloproteinases and NF-kappaB activation in rabbit synovial fibroblasts via E prostaglandins and Erk: Contrasting effects of nabumetone and 6MNA. Br J Pharmacol. 142:973–982. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Tao K, Zeng H, Xiao DM, Xiong A, Weng J and Kang B: Influences of IL-6R antibody on PMMA bone cement-mediated expression of OPG and RANKL in synovial fibroblasts. J Huazhong Univ Sci Technolog Med Sci. 34:241–246. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Fiorito S, Magrini L, Adrey J, Mailhé D and Brouty-Boyé D: Inflammatory status and cartilage regenerative potential of synovial fibroblasts from patients with osteoarthritis and chondropathy. Rheumatology (Oxford). 44:164–171. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Pierer M, Brentano F, Rethage J, Wagner U, Hantzschel H, Gay RE, Gay S and Kyburz D: The TNF superfamily member LIGHT contributes to survival and activation of synovial fibroblasts in rheumatoid arthritis. Rheumatology (Oxford). 46:1063–1070. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Scian R, Barrionuevo P, Rodriguez AM, Arriola Benitez PC, García Samartino C, Fossati CA, Giambartolomei GH and Delpino MV: Brucella abortus invasion of synoviocytes inhibits apoptosis and induces bone resorption through RANKL expression. Infect Immun. 81:1940–1951. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Nikitopoulou I, Oikonomou N, Karouzakis E, Sevastou I, Nikolaidou-Katsaridou N, Zhao Z, Mersinias V, Armaka M, Xu Y, Masu M, et al: Autotaxin expression from synovial fibroblasts is essential for the pathogenesis of modeled arthritis. J Exp Med. 209:925–933. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Ohki E, Suzuki M, Aoe T, Ikawa Y, Negishi E and Ueno K: Expression of histamine H4 receptor in synovial cells from rheumatoid arthritic patients. Biol Pharm Bull. 30:2217–2220. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Brühl H, Mack M, Niedermeier M, Lochbaum D, Schölmerich J and Straub RH: Functional expression of the chemokine receptor CCR7 on fibroblast-like synoviocytes. Rheumatology (Oxford). 47:1771–1774. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Zimmermann T, Kunisch E, Pfeiffer R, Hirth A, Stahl HD, Sack U, Laube A, Liesaus E, Roth A, Palombo-Kinne E, et al: Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture–primary culture cells markedly differ from fourth-passage cells. Arthritis Res. 3:72–76. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Saalbach A, Aneregg U, Bruns M, Schnabel E, Herrmann K and Haustein UF: Novel fibroblast-specific monoclonal antibodies: Properties and specificities. J Invest Dermatol. 106:1314–1319. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Rosengren S, Boyle DL and Firestein GS: Acquisition, culture, and phenotyping of synovial fibroblasts. Methods Mol Med. 135:365–375. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Ouyang Q, Hu Z, Huang Q, Wu J, Wang R and Yang M: A protocol for the culture and isolation of murine synovial fibroblasts. Biomed Rep 5: 171-175, 2016.
APA
Zhao, J., Ouyang, Q., Hu, Z., Huang, Q., Wu, J., Wang, R., & Yang, M. (2016). A protocol for the culture and isolation of murine synovial fibroblasts. Biomedical Reports, 5, 171-175. https://doi.org/10.3892/br.2016.708
MLA
Zhao, J., Ouyang, Q., Hu, Z., Huang, Q., Wu, J., Wang, R., Yang, M."A protocol for the culture and isolation of murine synovial fibroblasts". Biomedical Reports 5.2 (2016): 171-175.
Chicago
Zhao, J., Ouyang, Q., Hu, Z., Huang, Q., Wu, J., Wang, R., Yang, M."A protocol for the culture and isolation of murine synovial fibroblasts". Biomedical Reports 5, no. 2 (2016): 171-175. https://doi.org/10.3892/br.2016.708
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Ouyang Q, Hu Z, Huang Q, Wu J, Wang R and Yang M: A protocol for the culture and isolation of murine synovial fibroblasts. Biomed Rep 5: 171-175, 2016.
APA
Zhao, J., Ouyang, Q., Hu, Z., Huang, Q., Wu, J., Wang, R., & Yang, M. (2016). A protocol for the culture and isolation of murine synovial fibroblasts. Biomedical Reports, 5, 171-175. https://doi.org/10.3892/br.2016.708
MLA
Zhao, J., Ouyang, Q., Hu, Z., Huang, Q., Wu, J., Wang, R., Yang, M."A protocol for the culture and isolation of murine synovial fibroblasts". Biomedical Reports 5.2 (2016): 171-175.
Chicago
Zhao, J., Ouyang, Q., Hu, Z., Huang, Q., Wu, J., Wang, R., Yang, M."A protocol for the culture and isolation of murine synovial fibroblasts". Biomedical Reports 5, no. 2 (2016): 171-175. https://doi.org/10.3892/br.2016.708
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