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Article

Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs

  • Authors:
    • Hao Tao
    • Yazhou Lin
    • Guoqing Zhang
    • Rui Gu
    • Bohua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing 100068, P.R. China
  • Pages: 357-360
    |
    Published online on: August 1, 2016
       https://doi.org/10.3892/br.2016.731
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Abstract

Allogeneic bone marrow mesenchymal stem cell (BMSC) transplantation has been investigated worldwide. However, few reports have addressed the survival status of human BMSCs in the intervertebral discs (IVDs) in vivo following transplantation. The current study aimed to observe the survival status of human BMSCs in rabbit IVDs. The IVDs of 15 New Zealand white rabbits were divided into three groups: Punctured blank control group (L1‑2); punctured physiological saline control group (L2‑3); and punctured human BMSCs transfected with green fluorescent protein (GFP) group (L3‑4, L4‑5 and L5‑6). One, 2, 4, 6 and 8 weeks after transplantation the IVDs were removed and a fluorescence microscope was used to observe the density of GFP‑positive human BMSCs. The results indicated that in the sections of specimens removed at 1, 2, 4, 6 and 8 weeks post‑transplantation, no GFP‑positive cells were observed in the control groups, whereas GFP‑positive cells were apparent in the nucleus pulposus at all periods in the GFP‑labeled human BMSCs group, and the cell density at 6 and 8 weeks was significantly less than that at 1, 2 and 4 weeks post‑transplantation (P<0.001). Thus, it was identified that human BMSCs were able to survive in the rabbit IVDs for 8 weeks.
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Copy and paste a formatted citation
Spandidos Publications style
Tao H, Lin Y, Zhang G, Gu R and Chen B: Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs. Biomed Rep 5: 357-360, 2016.
APA
Tao, H., Lin, Y., Zhang, G., Gu, R., & Chen, B. (2016). Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs. Biomedical Reports, 5, 357-360. https://doi.org/10.3892/br.2016.731
MLA
Tao, H., Lin, Y., Zhang, G., Gu, R., Chen, B."Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs". Biomedical Reports 5.3 (2016): 357-360.
Chicago
Tao, H., Lin, Y., Zhang, G., Gu, R., Chen, B."Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs". Biomedical Reports 5, no. 3 (2016): 357-360. https://doi.org/10.3892/br.2016.731
Copy and paste a formatted citation
x
Spandidos Publications style
Tao H, Lin Y, Zhang G, Gu R and Chen B: Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs. Biomed Rep 5: 357-360, 2016.
APA
Tao, H., Lin, Y., Zhang, G., Gu, R., & Chen, B. (2016). Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs. Biomedical Reports, 5, 357-360. https://doi.org/10.3892/br.2016.731
MLA
Tao, H., Lin, Y., Zhang, G., Gu, R., Chen, B."Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs". Biomedical Reports 5.3 (2016): 357-360.
Chicago
Tao, H., Lin, Y., Zhang, G., Gu, R., Chen, B."Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs". Biomedical Reports 5, no. 3 (2016): 357-360. https://doi.org/10.3892/br.2016.731
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