Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling

  • Authors:
    • Feijia Ding
    • Yunhong Li
    • Xiaolin Hou
    • Rui Zhang
    • Shuting Hu
    • Yin Wang
  • View Affiliations

  • Published online on: October 11, 2016     https://doi.org/10.3892/br.2016.776
  • Pages: 623-628
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Oxymatrine (OMT) is an alkaloid extracted from Sophora flavescens, which has broad anti-inflammatory, antitumor and immunosuppressant actions. However, the underlying molecular mechanisms have remained elusive. Heat shock protein 60 (HSP60) has recently been shown to have an important role in autoimmune reactions. The present study aimed to investigate whether OMT exerts its anti‑inflammatory effects by inhibiting microglial activation and examined the role of HSP60 in this process. Western blot analysis and ELISA showed that OMT decreased the expression and release of HSP60 by LPS‑activated BV2 cells. The expression of heat shock factor 1, the transcription factor of HSP60, was also suppressed by OMT. Extracellular HSP60 has been previously indicated to induce microglial apoptosis through the Toll‑like receptor (TLR)‑4 pathway. Flow cytometric analysis demonstrated that LPS treatment induced apoptosis of BV2 cells, which was inhibited by OMT in parallel with inhibition of LPS‑induced expression of TLR‑4. Furthermore, OMT was shown to suppress the levels of myeloid differentiation factor (MYD)88, nuclear factor (NF)‑κB, caspase‑3, inducible nitric oxide synthase, tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. In light of these results, it was concluded that OMT may exert its neuroprotective effects via HSP60/TLR‑4/MYD88/NF‑κB signaling pathways to inhibit microglial activation. OMT may therefore offer substantial therapeutic potential for treating neurodegenerative diseases associated with microglial activation.
View Figures
View References

Related Articles

Journal Cover

November-2016
Volume 5 Issue 5

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ding F, Li Y, Hou X, Zhang R, Hu S and Wang Y: Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling. Biomed Rep 5: 623-628, 2016
APA
Ding, F., Li, Y., Hou, X., Zhang, R., Hu, S., & Wang, Y. (2016). Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling. Biomedical Reports, 5, 623-628. https://doi.org/10.3892/br.2016.776
MLA
Ding, F., Li, Y., Hou, X., Zhang, R., Hu, S., Wang, Y."Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling". Biomedical Reports 5.5 (2016): 623-628.
Chicago
Ding, F., Li, Y., Hou, X., Zhang, R., Hu, S., Wang, Y."Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling". Biomedical Reports 5, no. 5 (2016): 623-628. https://doi.org/10.3892/br.2016.776