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Article

Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene

  • Authors:
    • Majid Zarrin
    • Farzaneh Ganj
    • Sama Faramarzi
  • View Affiliations / Copyright

    Affiliations: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran, Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran
  • Pages: 705-708
    |
    Published online on: October 18, 2016
       https://doi.org/10.3892/br.2016.783
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Abstract

Fusarium species are well-known plant pathogens and food contaminants that have also appeared as one of the most important groups of medically significant fungi. The sequences of the translation elongation factor (TEF)-1α gene have been broadly employed for species detection. A total of 50 strains of Fusarium spp., including environmental, clinical and reference isolates were used for the current study. The primer sets, Fu3f and Fu3r, were used to amplify an ~420-bp DNA fragment of the TEF-1α gene. Double digestion with two restriction enzymes, XhoI and SduI was used for discrimination of the Fusarium species in the TEF-1α gene fragment. Double digestion of the TEF-1α gene fragment from five clinically important Fusarium species were clearly differentiated from each other: The F. solani species complex, F. oxysporum species complex, F. verticillioides, F. proliferatum and F. fujikuroi. This method facilitates detection and enables verification of the Fusarium genus; therefore, it may be applied for disease control.
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Copy and paste a formatted citation
Spandidos Publications style
Zarrin M, Ganj F and Faramarzi S: Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene. Biomed Rep 5: 705-708, 2016.
APA
Zarrin, M., Ganj, F., & Faramarzi, S. (2016). Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene. Biomedical Reports, 5, 705-708. https://doi.org/10.3892/br.2016.783
MLA
Zarrin, M., Ganj, F., Faramarzi, S."Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene". Biomedical Reports 5.6 (2016): 705-708.
Chicago
Zarrin, M., Ganj, F., Faramarzi, S."Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene". Biomedical Reports 5, no. 6 (2016): 705-708. https://doi.org/10.3892/br.2016.783
Copy and paste a formatted citation
x
Spandidos Publications style
Zarrin M, Ganj F and Faramarzi S: Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene. Biomed Rep 5: 705-708, 2016.
APA
Zarrin, M., Ganj, F., & Faramarzi, S. (2016). Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene. Biomedical Reports, 5, 705-708. https://doi.org/10.3892/br.2016.783
MLA
Zarrin, M., Ganj, F., Faramarzi, S."Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene". Biomedical Reports 5.6 (2016): 705-708.
Chicago
Zarrin, M., Ganj, F., Faramarzi, S."Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene". Biomedical Reports 5, no. 6 (2016): 705-708. https://doi.org/10.3892/br.2016.783
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