Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
December-2016 Volume 5 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2016 Volume 5 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts

  • Authors:
    • Shingo Kainuma
    • Haruhiko Tokuda
    • Kazuhiko Fujita
    • Tetsu Kawabata
    • Go Sakai
    • Rie Matsushima‑Nishiwaki
    • Atsushi Harada
    • Osamu Kozawa
    • Takanobu Otsuka
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Orthopedic Surgery, National Center for Geriatrics and Gerontology, Obu, Aichi 474‑8511, Japan
  • Pages: 771-775
    |
    Published online on: November 1, 2016
       https://doi.org/10.3892/br.2016.798
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Incretins, the polypeptide hormone glucose‑ dependent insulinotropic polypeptide (GIP) and glucagon‑like peptide-1 (GLP-1) secreted from the small intestine after nutrient ingestion, are generally known to stimulate insulin secretion from pancreatic β-cells. We previously demonstrated that triiodothyronine (T3) stimulates osteocalcin synthesis at least in part through p38 mitogen‑activated protein kinase in osteoblast‑like MC3T3‑E1 cells. In the present study, we investigated the effects of GIP and GLP‑1 on T3‑stimulated osteocalcin synthesis and the mechanism of action involved in MC3T3‑E1 cells. GIP and GLP‑1 markedly suppressed the T3‑stimulated osteocalcin release. GIP and GLP‑1 significantly attenuated the expression levels of osteocalcin mRNA induced by T3. The T3‑stimulated transactivation activity of the thyroid hormone‑responsive element was reduced by GIP and GLP‑1. These results suggest that incretins repressed the T3‑stimulated osteocalcin synthesis in osteoblast‑like MC3T3‑E1 cells, and the suppressive effect of incretins was mediated through transcriptional levels.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Karsenty G and Wagner EF: Reaching a genetic and molecular understanding of skeletal development. Dev Cell. 2:389–406. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Boyce BF and Xing L: Functions of RANKL/RANK/OPG in bone modeling and remodeling. Arch Biochem Biophys. 473:139–146. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Hauschka PV, Lian JB, Cole DE and Gundberg CM: Osteocalcin and matrix Gla protein: Vitamin K-dependent proteins in bone. Physiol Rev. 69:990–1047. 1989.PubMed/NCBI

4 

Ducy P, Desbois C, Boyce B, Pinero G, Story B, Dunstan C, Smith E, Bonadio J, Goldstein S, Gundberg C, et al: Increased bone formation in osteocalcin-deficient mice. Nature. 382:448–452. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Karsenty G and Ferron M: The contribution of bone to whole-organism physiology. Nature. 481:314–320. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Gogakos AI, Bassett JH Duncan and Williams GR: Thyroid and bone. Arch Biochem Biophys. 503:129–136. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Vestergaard P and Mosekilde L: Hyperthyroidism, bone mineral, and fracture risk - a meta-analysis. Thyroid. 13:585–593. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Cheng SY, Leonard JL and Davis PJ: Molecular aspects of thyroid hormone actions. Endocr Rev. 31:139–170. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Mullur R, Liu YY and Brent GA: Thyroid hormone regulation of metabolism. Physiol Rev. 94:355–382. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Kasono K, Sato K, Han DC, Fujii Y, Tsushima T and Shizume K: Stimulation of alkaline phosphatase activity by thyroid hormone in mouse osteoblast-like cells (MC3T3-E1): A possible mechanism of hyperalkaline phosphatasia in hyperthyroidism. Bone Miner. 4:355–363. 1988.PubMed/NCBI

11 

Ishisaki A, Tokuda H, Yoshida M, Hirade K, Kunieda K, Hatakeyama D, Shibata T and Kozawa O: Activation of p38 mitogen-activated protein kinase mediates thyroid hormone- stimulated osteocalcin synthesis in osteoblasts. Mol Cell Endocrinol. 214:189–195. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Kanno Y, Ishisaki A, Yoshida M, Nakajima K, Tokuda H, Numata O and Kozawa O: Adenylyl cyclase-cAMP system inhibits thyroid hormone-stimulated osteocalcin synthesis in osteoblasts. Mol Cell Endocrinol. 229:75–82. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Baggio LL and Drucker DJ: Biology of incretins: GLP-1 and GIP. Gastroenterology. 132:2131–2157. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Holst JJ: The physiology of glucagon-like peptide 1. Physiol Rev. 87:1409–1439. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Bollag RJ, Zhong Q, Phillips P, Min L, Zhong L, Cameron R, Mulloy AL, Rasmussen H, Qin F, Ding KH, et al: Osteoblast- derived cells express functional glucose-dependent insulinotropic peptide receptors. Endocrinology. 141:1228–1235. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Bollag RJ, Zhong Q, Ding KH, Phillips P, Zhong L, Qin F, Cranford J, Mulloy AL, Cameron R and Isales CM: Glucose-dependent insulinotropic peptide is an integrative hormone with osteotropic effects. Mol Cell Endocrinol. 177:35–41. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Sanz C, Vázquez P, Blázquez C, Barrio PA, Alvarez MM and Blázquez E: Signaling and biological effects of glucagon-like peptide 1 on the differentiation of mesenchymal stem cells from human bone marrow. Am J Physiol Endocrinol Metab. 298:E634–E643. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Sudo H, Kodama HA, Amagai Y, Yamamoto S and Kasai S: In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI

19 

Kozawa O, Tokuda H, Miwa M, Kotoyori J and Oiso Y: Cross-talk regulation between cyclic AMP production and phosphoinositide hydrolysis induced by prostaglandin E2 in osteoblast-like cells. Exp Cell Res. 198:130–134. 1992. View Article : Google Scholar : PubMed/NCBI

20 

Zhang W, Yang N and Shi XM: Regulation of mesenchymal stem cell osteogenic differentiation by glucocorticoid-induced leucine zipper (GILZ). J Biol Chem. 283:4723–4729. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Simpson DA, Feeney S, Boyle C and Stitt AW: Retinal VEGF mRNA measured by SYBR green I fluorescence: A versatile approach to quantitative PCR. Mol Vis. 6:178–183. 2000.PubMed/NCBI

22 

Seino Y and Yabe D: Glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1: Incretin actions beyond the pancreas. J Diabetes Investig. 4:108–130. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Godinho RO, Duarte T and Pacini ES: New perspectives in signaling mediated by receptors coupled to stimulatory G protein: The emerging significance of cAMP effux and extracellular cAMP-adenosine pathway. Front Pharmacol. 6:582015. View Article : Google Scholar : PubMed/NCBI

24 

Lecka-Czernik B: Safety of anti-diabetic therapies on bone. Clin Rev Bone Miner Metab. 11:49–58. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Lee NK and Karsenty G: Reciprocal regulation of bone and energy metabolism. Trends Endocrinol Metab. 19:161–166. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kainuma S, Tokuda H, Fujita K, Kawabata T, Sakai G, Matsushima‑Nishiwaki R, Harada A, Kozawa O and Otsuka T: Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts. Biomed Rep 5: 771-775, 2016.
APA
Kainuma, S., Tokuda, H., Fujita, K., Kawabata, T., Sakai, G., Matsushima‑Nishiwaki, R. ... Otsuka, T. (2016). Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts. Biomedical Reports, 5, 771-775. https://doi.org/10.3892/br.2016.798
MLA
Kainuma, S., Tokuda, H., Fujita, K., Kawabata, T., Sakai, G., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts". Biomedical Reports 5.6 (2016): 771-775.
Chicago
Kainuma, S., Tokuda, H., Fujita, K., Kawabata, T., Sakai, G., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts". Biomedical Reports 5, no. 6 (2016): 771-775. https://doi.org/10.3892/br.2016.798
Copy and paste a formatted citation
x
Spandidos Publications style
Kainuma S, Tokuda H, Fujita K, Kawabata T, Sakai G, Matsushima‑Nishiwaki R, Harada A, Kozawa O and Otsuka T: Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts. Biomed Rep 5: 771-775, 2016.
APA
Kainuma, S., Tokuda, H., Fujita, K., Kawabata, T., Sakai, G., Matsushima‑Nishiwaki, R. ... Otsuka, T. (2016). Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts. Biomedical Reports, 5, 771-775. https://doi.org/10.3892/br.2016.798
MLA
Kainuma, S., Tokuda, H., Fujita, K., Kawabata, T., Sakai, G., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts". Biomedical Reports 5.6 (2016): 771-775.
Chicago
Kainuma, S., Tokuda, H., Fujita, K., Kawabata, T., Sakai, G., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Attenuation by incretins of thyroid hormone-stimulated osteocalcin synthesis in osteoblasts". Biomedical Reports 5, no. 6 (2016): 771-775. https://doi.org/10.3892/br.2016.798
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team