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Article

Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells

  • Authors:
    • Mohammad J. Alkhatatbeh
    • Lisa F. Lincz
    • Rick F. Thorne
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan, Hunter Haematology Research Group, Calvary Mater Newcastle Hospital, Waratah, NSW 2298, Australia, School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia
  • Pages: 63-68
    |
    Published online on: November 9, 2016
       https://doi.org/10.3892/br.2016.806
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Abstract

Plasma microparticles (MPs) are heterogeneously sized submicron extracellular vesicles that originate from the cell membrane as a result of cell activation or apoptosis. Circulating MPs express cell‑specific molecules that reflect their cell of origin and they are increasingly investigated for their potential role in intercellular communication. The aim of the current study was to determine if size exclusion chromatography could be used to purify fluorescent‑labeled MPs in sufficient concentrations to be used experimentally in cell binding assays. Bio‑maleimide was used to stain plasma MPs in platelet free plasma before applying to size exclusion chromatography. Collected fractions were analyzed for protein content and MPs were enumerated by flow cytometry. Fractions were ultracentrifuged and MPs further confirmed by western blotting for the putative diabetic marker, cluster of differentiation (CD)36 and platelet‑specific CD41 proteins. Fractions that contained MPs were incubated with HK2 cells to determine MP‑cell binding. Bio‑maleimide‑stained MPs were detected across various fractions of size exclusion, and pellets of these fractions confirmed positivity for the MP markers, CD41 and CD36. The addition of the isolated MPs to HK2 renal tubular cells and analysis by epi‑fluorescent imaging demonstrated that, in principle, the labeled MPs are able to bind to cells in vitro. Notably, only the first eluted MP fraction bound HK2 cells indicating a possible association between MP size and cell‑targeting properties.
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1 

Hargett LA and Bauer NN: On the origin of microparticles: From “platelet dust” to mediators of intercellular communication. Pulm Circ. 3:329–340. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Yáñez-Mó M, Siljander PR, Andreu Z, Zavec AB, Borràs FE, Buzas EI, Buzas K, Casal E, Cappello F, Carvalho J, et al: Biological properties of extracellular vesicles and their physiological functions. J Extracell Vesicles. 4:270662015. View Article : Google Scholar : PubMed/NCBI

3 

Nielsen MH, Beck-Nielsen H, Andersen MN and Handberg A: A flow cytometric method for characterization of circulating cell-derived microparticles in plasma. J Extracell Vesicles. 3:Feb 4–2014.(Epub ahead of print). http://dx.doi.org/10.3402/jev.v3.20795PubMed/NCBI

4 

Arraud N, Linares R, Tan S, Gounou C, Pasquet JM, Mornet S and Brisson AR: Extracellular vesicles from blood plasma: Determination of their morphology, size, phenotype and concentration. J Thromb Haemost. 12:614–627. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Wolf P: The nature and significance of platelet products in human plasma. Br J Haematol. 13:269–288. 1967. View Article : Google Scholar : PubMed/NCBI

6 

Lynch SF and Ludlam CA: Plasma microparticles and vascular disorders. Br J Haematol. 137:36–48. 2007.PubMed/NCBI

7 

Horstman LL, Jy W, Jimenez JJ and Ahn YS: Endothelial microparticles as markers of endothelial dysfunction. Front Biosci. 9:1118–1135. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Bernard S, Loffroy R, Sérusclat A, Boussel L, Bonnefoy E, Thévenon C, Rabilloud M, Revel D, Moulin P and Douek P: Increased levels of endothelial microparticles CD144 (VE-Cadherin) positives in type 2 diabetic patients with coronary noncalcified plaques evaluated by multidetector computed tomography (MDCT). Atherosclerosis. 203:429–435. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Alkhatatbeh MJ, Enjeti AK, Acharya S, Thorne RF and Lincz LF: The origin of circulating CD36 in type 2 diabetes. Nutr Diabetes. 3:e592013. View Article : Google Scholar : PubMed/NCBI

10 

Jaiswal R, Grau GE Raymond and Bebawy M: Cellular communication via microparticles: Role in transfer of multidrug resistance in cancer. Future Oncol. 10:655–669. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Antonyak MA and Cerione RA: Microvesicles as mediators of intercellular communication in cancer. Methods Mol Biol. 1165:147–173. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Jansen F, Yang X, Hoyer FF, Paul K, Heiermann N, Becher MU, Abu Hussein N, Kebschull M, Bedorf J, Franklin BS, et al: Endothelial microparticle uptake in target cells is annexin I/phosphatidylserine receptor dependent and prevents apoptosis. Arterioscler Thromb Vasc Biol. 32:1925–1935. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Fernandez-Martínez AB, Torija AV, Carracedo J, Ramirez R and de Lucio-Cazaña FJ: Microparticles released by vascular endothelial cells increase hypoxia inducible factor expression in human proximal tubular HK-2 cells. Int J Biochem Cell Biol. 53:334–342. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Alkhatatbeh MJ, Mhaidat NM, Enjeti AK, Lincz LF and Thorne RF: The putative diabetic plasma marker, soluble CD36, is non-cleaved, non-soluble and entirely associated with microparticles. J Thromb Haemost. 9:844–851. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Thorne RF, Zhang X, Song C, Jin B and Burns GF: Novel immunoblotting monoclonal antibodies against human and rat CD36/fat used to identify an isoform of CD36 in rat muscle. DNA Cell Biol. 25:302–311. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Enjeti AK, Lincz L and Seldon M: Bio-maleimide as a generic stain for detection and quantitation of microparticles. Int J Lab Hematol. 30:196–199. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Robert S, Poncelet P, Lacroix R, Arnaud L, Giraudo L, Hauchard A, Sampol J and Dignat-George F: Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: A first step towards multicenter studies? J Thromb Haemost. 7:190–197. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Lacroix R, Robert S, Poncelet P, Kasthuri RS, Key NS and Dignat-George F: ISTH SSC Workshop: Standardization of platelet-derived microparticle enumeration by flow cytometry with calibrated beads: Results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. J Thromb Haemost. 8:2571–2574. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Sanchez-Niño MD, Fernandez-Fernandez B, Perez-Gomez MV, Poveda J, Sanz AB, Cannata-Ortiz P, Ruiz-Ortega M, Egido J, Selgas R and Ortiz A: Albumin-induced apoptosis of tubular cells is modulated by BASP1. Cell Death Dis. 6:e16442015. View Article : Google Scholar : PubMed/NCBI

20 

de Menezes-Neto A, Sáez MJ, Lozano-Ramos I, Segui-Barber J, Martin-Jaular L, Ullate JM, Fernandez-Becerra C, Borrás FE and Del Portillo HA: Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. J Extracell Vesicles. 4:273782015. View Article : Google Scholar : PubMed/NCBI

21 

Baranyai T, Herczeg K, Onódi Z, Voszka I, Módos K, Marton N, Nagy G, Mäger I, Wood MJ, El Andaloussi S, et al: Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods. PLoS One. 10:e01456862015. View Article : Google Scholar : PubMed/NCBI

22 

Böing AN, van der Pol E, Grootemaat AE, Coumans FA, Sturk A and Nieuwland R: Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 3:Sep 8–2014.http://dx.doi.org/10.3402/jev.v3.23430 View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Alkhatatbeh M , Lincz LF and Thorne RF: Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells. Biomed Rep 6: 63-68, 2017.
APA
Alkhatatbeh, M. ., Lincz, L.F., & Thorne, R.F. (2017). Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells. Biomedical Reports, 6, 63-68. https://doi.org/10.3892/br.2016.806
MLA
Alkhatatbeh, M. ., Lincz, L. F., Thorne, R. F."Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells". Biomedical Reports 6.1 (2017): 63-68.
Chicago
Alkhatatbeh, M. ., Lincz, L. F., Thorne, R. F."Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells". Biomedical Reports 6, no. 1 (2017): 63-68. https://doi.org/10.3892/br.2016.806
Copy and paste a formatted citation
x
Spandidos Publications style
Alkhatatbeh M , Lincz LF and Thorne RF: Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells. Biomed Rep 6: 63-68, 2017.
APA
Alkhatatbeh, M. ., Lincz, L.F., & Thorne, R.F. (2017). Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells. Biomedical Reports, 6, 63-68. https://doi.org/10.3892/br.2016.806
MLA
Alkhatatbeh, M. ., Lincz, L. F., Thorne, R. F."Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells". Biomedical Reports 6.1 (2017): 63-68.
Chicago
Alkhatatbeh, M. ., Lincz, L. F., Thorne, R. F."Bio-maleimide-stained plasma microparticles can be purified in a native state and target human proximal tubular HK2 cells". Biomedical Reports 6, no. 1 (2017): 63-68. https://doi.org/10.3892/br.2016.806
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