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Article

Antiproliferative effect of berberine on canine mammary gland cancer cell culture

  • Authors:
    • Reyhaneh Sefidabi
    • Pejman Mortazavi
    • Saeed Hosseini
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran
  • Pages: 95-98
    |
    Published online on: November 11, 2016
       https://doi.org/10.3892/br.2016.809
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Abstract

Canine mammary gland tumors are the most frequent cause of cancer in female dogs. Numerous studies using cancer cell lines and clinical trials have indicated that various natural products and antioxidants reduce or possibly prevent the development of cancer. Berberine (BBR), the most important alkaloid in the Berberidaceae, which exerts a wide range of pharmacological and biochemical effects, has drawn much attention due to its particularly high antitumor activity in vitro and in animal studies. The aim of the present study was to investigate the antiproliferative effect of BBR against a canine mammary gland carcinoma cell line (CF41.Mg) in vitro. CF41.Mg cells were cultured in RPMI‑1640 medium containing 10% heat inactived fetal bovine serum (FBS) and 100 mg/ml peniciline‑streptomycin. Subsequently the cells were treated with different concentrations of BBR chloride (10, 25, 50, 100 and 200 µM) at a density of 12,000 cells/well in 96‑well plates. Following treatment, the MTT assay was used to detect cell viability after 24‑, 48- and 72-h incubations at 37˚C with 5% CO2. The results indicated that BBR inhibited proliferation of canine mammary gland carcinoma cells, as treatment with 100 µM BBR for 24 h resulted in a significant decrease in cell viability (P<0.005). As the present study demonstrated that BBR (10‑200 µM) induced cancer cell death, it is proposed that BBR may serve as a candidate agent against canine mammary tumor cells via its antiproliferative activity.
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1 

Croce CM: Oncogenes and cancer. N Engl J Med. 358:502–511. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Chandra D: Mitochondria as Targets for Phytochemicals in Cancer Prevention and Therapy. Springer; New York, USA: pp. 62–64. 2013

3 

Cooper GM: The Cell: A Molecular Approach. 2nd. Sinauer Associates; Sunderland, MA: 2000

4 

Andrade FH, Figueiroa FC, Bersano PR, Bissacot DZ and Rocha NS: Malignant mammary tumor in female dogs: Environmental contaminants. Diagn Pathol. 5:452010. View Article : Google Scholar : PubMed/NCBI

5 

Casey SC, Amedei A, Aquilano K, Azmi AS, Benencia F, Bhakta D, Bilsland AE, Boosani CS, Chen S, Ciriolo MR, et al: Cancer prevention and therapy through the modulation of the tumor microenvironment. Semin Cancer Biol. 35:S199–S223. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Seifu D, Assefa F and Abay SM: Medical plants as antioxidant agents: Understanding their mechanism of action and therapeutic efficacy. Research Signpost; Kerala, India: pp. 97–145. 2012

7 

Efferth T, Miyachi H and Bartsch H: Pharmacogenomics of a traditional Japanese herbal medicine (Kampo) for cancer therapy. Cancer Genomics Proteomics. 4:81–91. 2007.PubMed/NCBI

8 

Hsu S, Yu FX, Huang Q, Lewis J, Singh B, Dickinson D, Borke J, Sharawy M, Wataha J, Yamamoto T, et al: A mechanism-based in vitro anticancer drug screening approach for phenolic phytochemicals. Assay Drug Dev Technol. 1:611–618. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Siriwatanametanon N, Fiebich BL, Efferth T, Prieto JM and Heinrich M: Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities. J Ethnopharmacol. 130:196–207. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Solowey E, Lichtenstein M, Sallon S, Paavilainen H, Solowey E and Lorberboum-Galski H: Evaluating medicinal plants for anticancer activity. ScientificWorldJournal. 2014:7214022014. View Article : Google Scholar : PubMed/NCBI

11 

Greenwell M and Rahman PK: Medicinal Plants: Their Use in Anticancer Treatment. Int J Pharm Sci Res. 6:4103–4112. 2015.PubMed/NCBI

12 

Gao S, Basu S, Yang G, Deb A and Hu M: Oral bioavailability challenges of natural products used in cancer chemoprevention. Pro Chem. 25:1553–1574. 2013.

13 

Choi MS, Yuk DY, Oh JH, Jung HY, Han SB, Moon DC and Hong JT: Berberine inhibits human neuroblastoma cell growth through induction of p53-dependent apoptosis. Anticancer Res 28 (6A). 3777–3784. 2008.

14 

King KL and Cidlowski JA: Cell cycle regulation and apoptosis. Annu Rev Physiol. 60:601–617. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Foster I: Cancer: A cell cycle defect. Radiography. 14:144–149. 2008. View Article : Google Scholar

16 

Thatte U and Dahanukar S: Apoptosis: Clinical relevance and pharmacological manipulation. Drugs. 54:511–532. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Pearson M, Carbone R, Sebastiani C, Cioce M, Fagioli M, Saito S, Higashimoto Y, Appella E, Minucci S, Pandolfi PP, et al: PML regulates p53 acetylation and premature senescence induced by oncogenic Ras. Nature. 406:207–210. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Vermeulen K, Berneman ZN and Van Bockstaele DR: Cell cycle and apoptosis. Cell Prolif. 36:165–175. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Hsu WH, Hsieh YS, Kuo HC, Teng CY, Huang HI, Wang CJ, Yang SF, Liou YS and Kuo WH: Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL. Arch Toxicol. 81:719–728. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Sun Y, Xun K, Wang Y and Chen X: A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs. Anticancer Drugs. 20:757–769. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Serafim TL, Oliveira PJ, Sardao VA, Perkins E, Parke D and Holy J: Different concentrations of berberine result in distinct cellular localization patterns and cell cycle effects in a melanoma cell line. Cancer Chemother Pharmacol. 61:1007–1018. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Bremer E, van Dam G, Kroesen BJ, de Leij L and Helfrich W: Targeted induction of apoptosis for cancer therapy: Current progress and prospects. Trends Mol Med. 12:382–393. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Yin X, Zhou J, Jie C, Xing D and Zhang Y: Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549. Life Sci. 75:2233–2244. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Krey AK and Hahn FE: Berberine: Complex with DNA. Science. 166:755–757. 1969. View Article : Google Scholar : PubMed/NCBI

25 

Meeran SM, Katiyar S and Katiyar SK: Berberine-induced apoptosis in human prostate cancer cells is initiated by reactive oxygen species generation. Toxicol Appl Pharmacol. 229:33–43. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Letasiová S, Jantová S, Cipák L and Múcková M: Berberine-antiproliferative activity in vitro and induction of apoptosis/necrosis of the U937 and B16 cells. Cancer Lett. 239:254–262. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Jantova S, Cipak L and Letasiova S: Berberine induces apoptosis through a mitochondrial/caspase pathway in human promonocytic U937 cells. Toxicol In Vitro. 21:25–31. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Król M, Pawłowski KM, Szyszko K, Maciejewski H, Dolka I, Manuali E, Jank M and Motyl T: The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture in vitro. BMC Vet Res. 8:352012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sefidabi R, Mortazavi P and Hosseini S: Antiproliferative effect of berberine on canine mammary gland cancer cell culture . Biomed Rep 6: 95-98, 2017.
APA
Sefidabi, R., Mortazavi, P., & Hosseini, S. (2017). Antiproliferative effect of berberine on canine mammary gland cancer cell culture . Biomedical Reports, 6, 95-98. https://doi.org/10.3892/br.2016.809
MLA
Sefidabi, R., Mortazavi, P., Hosseini, S."Antiproliferative effect of berberine on canine mammary gland cancer cell culture ". Biomedical Reports 6.1 (2017): 95-98.
Chicago
Sefidabi, R., Mortazavi, P., Hosseini, S."Antiproliferative effect of berberine on canine mammary gland cancer cell culture ". Biomedical Reports 6, no. 1 (2017): 95-98. https://doi.org/10.3892/br.2016.809
Copy and paste a formatted citation
x
Spandidos Publications style
Sefidabi R, Mortazavi P and Hosseini S: Antiproliferative effect of berberine on canine mammary gland cancer cell culture . Biomed Rep 6: 95-98, 2017.
APA
Sefidabi, R., Mortazavi, P., & Hosseini, S. (2017). Antiproliferative effect of berberine on canine mammary gland cancer cell culture . Biomedical Reports, 6, 95-98. https://doi.org/10.3892/br.2016.809
MLA
Sefidabi, R., Mortazavi, P., Hosseini, S."Antiproliferative effect of berberine on canine mammary gland cancer cell culture ". Biomedical Reports 6.1 (2017): 95-98.
Chicago
Sefidabi, R., Mortazavi, P., Hosseini, S."Antiproliferative effect of berberine on canine mammary gland cancer cell culture ". Biomedical Reports 6, no. 1 (2017): 95-98. https://doi.org/10.3892/br.2016.809
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