Synthetic polyphosphate inhibits endogenous coagulation and platelet aggregation in vitro

Yang,Xiaoyang; Wan,Mengjie; Liang,Ting; Peng,Minyuan; Chen,Fangping

doi:10.3892/br.2016.816

January-2017 Volume 6 Issue 1

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January-2017 Volume 6 Issue 1

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Article

Synthetic polyphosphate inhibits endogenous coagulation and platelet aggregation in vitro

Authors:
- Xiaoyang Yang
- Mengjie Wan
- Ting Liang
- Minyuan Peng
- Fangping Chen
View Affiliations / Copyright

Affiliations: Department of Hematology, Haikou Municipal Hospital, Affiliated Haikou Hospital of Central South University, Haikou, Hainan 570208, P.R. China, Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Pages: 57-62
|
Published online on: November 23, 2016

https://doi.org/10.3892/br.2016.816
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Abstract

Platelet-derived polyphosphate has previously been indicated to induce coagulation. However, industrially synthesized polyphosphate has been found to have different effects from those of the platelet-derived form. The present study investigated whether synthetic sodium polyphosphate inhibits coagulation using routine coagulation tests and thromboelastography. Synthetic polyphosphate was found to inhibit adenosine diphosphate-, epinephrine-, arachidonic acid-, ristocetin-, thrombin-, oxytocin- and pituitrin-induced platelet aggregation. The effects of synthetic polyphosphate in clotting inhibition were revealed by the analysis of clotting factor activity and platelet aggregation tests. Synthetic polyphosphate may inhibit platelet aggregation by reducing platelet calcium levels, as indicated by the results of flow cytometric analysis and high-throughput fluorescent screening. Furthermore, analysis of thromboxane (TX)B2 by ELISA indicated that synthetic polyphosphate reduces platelet aggregation by inhibiting the TXA2 signaling pathway. In conclusion, synthetic polyphosphate inhibits clotting factor activity and endogenous coagulation by reducing the levels of calcium ions and TXA2 to curb platelet aggregation.

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1	Brown MR and Kornberg A: Inorganic polyphosphate in the origin and survival of species. Proc Natl Acad Sci USA. 101:16085–16087. 2004. View Article : Google Scholar : PubMed/NCBI
2	Schwartz C, Jones KK, Mack TW and Vance RW: The use of sodium metaphosphate for the preparation of soft-curd milk. J Dairy Sci. 23:19–35. 1940. View Article : Google Scholar
3	Wiame JM: Yeast metaphosphate. Fed Proc. 6:3021947.PubMed/NCBI
4	Jimenez-Nuñez MD, Moreno-Sanchez D, Hernandez-Ruiz L, Benítez-Rondán A, Ramos-Amaya A, Rodríguez-Bayona B, Medina F, Brieva JA and Ruiz FA: Myeloma cells contain high levels of inorganic polyphosphate which is associated with nucleolar transcription. Haematologica. 97:1264–1271. 2012. View Article : Google Scholar : PubMed/NCBI
5	Faxälv L, Boknäs N, Ström JO, Tengvall P, Theodorsson E, Ramström S and Lindahl TL: Putting polyphosphates to the test: evidence against platelet-induced activation of factor XII. Blood. 122:3818–3824. 2013. View Article : Google Scholar : PubMed/NCBI
6	Smith SA, Choi SH, Davis-Harrison R, Huyck J, Boettcher J, Rienstra CM and Morrissey JH: Polyphosphate exerts differential effects on blood clotting, depending on polymer size. Blood. 116:4353–4359. 2010. View Article : Google Scholar : PubMed/NCBI
7	Yang X, Wan M, Yang K and Chen F: Long chain polyphosphates identified in infectious fever patients in the department of hematology. Acta Med Mediterr. 32:377–383. 2016.
8	Levi M, Toh CH, Thachil J and Watson HG: British Committee for Standards in Haematology: Guidelines for the diagnosis and management of disseminated intravascular coagulation. Br J Haematol. 145:24–33. 2009. View Article : Google Scholar : PubMed/NCBI
9	Kol A and Borjesson DL: Application of thrombelastography/thromboelastometry to veterinary medicine. Vet Clin Pathol. 39:405–416. 2010. View Article : Google Scholar : PubMed/NCBI
10	do Céu Monteiro M, Sansonetty F, Gonçalves MJ and O'Connor JE: Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41. Cytometry. 35:302–310. 1999. View Article : Google Scholar : PubMed/NCBI
11	Walsh TG, Harper MT and Poole AW: SDF-1α is a novel autocrine activator of platelets operating through its receptor CXCR4. Cell Signal. 27:37–46. 2015. View Article : Google Scholar : PubMed/NCBI
12	Wada H, Matsumoto T and Yamashita Y: Diagnosis and treatment of disseminated intravascular coagulation (DIC) according to four DIC guidelines. J Intensive Care. 2:152014. View Article : Google Scholar : PubMed/NCBI
13	Lorenz B and Schröder HC: Methods for investigation of inorganic polyphosphates and polyphosphate-metabolizing enzymes. Prog Mol Subcell Biol. 23:217–239. 1999. View Article : Google Scholar : PubMed/NCBI
14	Smith SA, Mutch NJ, Baskar D, Rohloff P, Docampo R and Morrissey JH: Polyphosphate modulates blood coagulation and fibrinolysis. Proc Natl Acad Sci USA. 103:903–908. 2006. View Article : Google Scholar : PubMed/NCBI
15	Katz-Summercorn AC, Cuffolo G, Hossain MA and Wilde M: The use of rapid thromboelastogram for trauma mortality prediction. Am J Surg. 208:3162014. View Article : Google Scholar : PubMed/NCBI
16	Tanriverdi S, Koroglu OA, Uygur O, Balkan C, Yalaz M and Kultursay N: The effect of inhaled nitric oxide therapy on thromboelastogram in newborns with persistent pulmonary hypertension. Eur J Pediatr. 173:1381–1385. 2014. View Article : Google Scholar : PubMed/NCBI
17	Valeri CR and Ragno G: In vitro testing of platelets using the thromboelastogram, platelet function analyzer, and the clot signature analyzer to predict the bleeding time. Transfus Apheresis Sci. 35:33–41. 2006. View Article : Google Scholar
18	Fontana P, Zufferey A, Daali Y and Reny JL: Antiplatelet therapy: targeting the TxA2 pathway. J Cardiovasc Transl Res. 7:29–38. 2014. View Article : Google Scholar : PubMed/NCBI
19	Lordkipanidzé M, Pharand C, Schampaert E, Turgeon J, Palisaitis DA and Diodati JG: A comparison of six major platelet function tests to determine the prevalence of aspirin resistance in patients with stable coronary artery disease. Eur Heart J. 28:1702–1708. 2007. View Article : Google Scholar : PubMed/NCBI
20	Sangkuhl K, Shuldiner AR, Klein TE and Altman RB: Platelet aggregation pathway. Pharmacogenet Genomics. 21:516–521. 2011. View Article : Google Scholar : PubMed/NCBI