Gaseous nitric oxide for the local treatment of bacterial keratitis in mice

Deichelbohrer,Mona; Wu,Ming‑Feng; Seitz,Berthold; Wagenpfeil,Stefan; Meier,Carola; Bischoff,Markus; Tschernig,Thomas

doi:10.3892/br.2016.821

January-2017 Volume 6 Issue 1

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January-2017 Volume 6 Issue 1

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Article

Gaseous nitric oxide for the local treatment of bacterial keratitis in mice

Authors:
- Mona Deichelbohrer
- Ming‑Feng Wu
- Berthold Seitz
- Stefan Wagenpfeil
- Carola Meier
- Markus Bischoff
- Thomas Tschernig
View Affiliations / Copyright

Affiliations: Institute of Anatomy and Cell Biology, Saarland University Faculty of Medicine, Homburg, D‑66424 Saar, Germany, Department of Ophthalmology, Saarland University Medical Center, Homburg, D‑66424 Saar, Germany, Institute for Medical Biometry, Epidemiology and Medical Informatics, Saarland University, Homburg, D‑66424 Saar, Germany, Institute of Medical Microbiology and Hygiene, Saarland University Faculty of Medicine, Homburg, D‑66424 Saar, Germany
Pages: 75-78
|
Published online on: November 30, 2016

https://doi.org/10.3892/br.2016.821
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Abstract

The successful treatment of severe bacterial keratitis continues to be a challenge in animals and humans. In the present study the aim was to assess gaseous therapy using gaseous nitric oxide (gNO) in a murine model of Pseudomonas aeruginosa keratitis. The cornea of anesthetized mice was mechanically scratched and covered with a bacterial suspension of P. aeruginosa. One day later, the infected eyes were exposed to 200 ppm NO for 30 min. Three to seven days later the mice were sacrificed and the bulbi were obtained and processed for light microscopy. The read out parameter was the maximal corneal thickness and the severity of the hypopyon. The therapy with NO did not result in either a reduction of inflammation concerning the maximal corneal thickness or the severity of the hypopyon. The bacterial load was not investigated due to technical limitations. Thus, exposure to gNO did not reduce the local inflammation in P. aeruginosa induced murine keratitis at the investigated time‑points. This does not exclude effects of NO on the bacterial load, and in experimental and human keratitis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Gaseous nitric oxide for the local treatment of bacterial keratitis in mice

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