Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
February-2017 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2017 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis

  • Authors:
    • Wan‑Li Zuo
    • Jie‑Min Zhao
    • Ji‑Xiong Huang
    • Wei Zhou
    • Ze‑Hong Lei
    • Yan‑Ming Huang
    • Yan‑Fen Huang
    • Hai‑Gang Li
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Jiangmen Central Hospital, The Jiangmen Affiliated Hospital of Sun Yat‑sen University, Jiangmen, Guangdong 529030, P.R. China, Department of Pathology, The Jiangmen Central Hospital, The Jiangmen Affiliated Hospital of Sun Yat‑sen University, Jiangmen, Guangdong 529030, P.R. China, Department of Medical Laboratory, The Jiangmen Central Hospital, The Jiangmen Affiliated Hospital of Sun Yat‑sen University, Jiangmen, Guangdong 529030, P.R. China, Department of Pathology, Zengcheng People's Hospital, Guangzhou, Guangdong 511300, P.R. China
  • Pages: 201-205
    |
    Published online on: December 29, 2016
       https://doi.org/10.3892/br.2016.832
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Pulmonary fibrosis (PF) is a life‑threatening non‑tumorous disease characterized by progressive fibrosis and worsening lung function. Various drugs, such as bleomycin, can contribute to lung injury and PF, with lung injury potentially occurring in 10% of bleomycin users. Bleomycin is the most commonly used drug in the establishment of an animal model of PF in rats. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) serve an important role in controlling tissue organization and fibrosis following injury. The present study examined the effect of bosentan on fibrotic lung tissue in rats administrated with bleomycin. In total, 48 Wistar rats were administrated with bleomycin, with or without bosentan, while the control rats received saline. The lung tissues were microscopically examined by staining with hematoxylin and eosin and Masson's trichome. ELISA was also used to detect the MMP‑9 and TIMP‑1 concentrations in the plasma. The results indicated that the bosentan‑treated groups on the next day and the 15th day showed significant reversal of pathological findings. In addition, the concentrations of MMP‑9 and TIMP‑1 appeared to be altered following bosentan treatment, improving the bleomycin‑induced PF. Masson's trichome staining showed high collagen deposition in the lung tissue sections, which may be a direct result of the activity of MMP‑9 and TIMP‑1. Furthermore, the deposition of collagen was significantly inhibited in bosentan‑treated groups. In conclusion, these results demonstrated that bosentan inhibited lung fibrosis induced by bleomycin and it may be used as an inhibitor of PF.
View Figures

Figure 1

View References

1 

Selman M, Buendía-Roldán I and Pardo A: Aging and pulmonary fibrosis. Rev Invest Clin. 68:75–83. 2016.PubMed/NCBI

2 

Hutchinson J, Fogarty A, Hubbard R and McKeever T: Global incidence and mortality of idiopathic pulmonary fibrosis: A systematic review. Eur Respir J. 46:795–806. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Antoniou KM, Wuyts W, Wijsenbeek M and Wells AU: Medical Therapy in Idiopathic Pulmonary Fibrosis. Semin Respir Crit Care Med. 37:368–377. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Lynch JP III, Huynh RH, Fishbein MC, Saggar R, Belperio JA and Weigt SS: Idiopathic Pulmonary Fibrosis: Epidemiology, Clinical Features, Prognosis, and Management. Semin Respir Crit Care Med. 37:331–357. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Vancheri C, Failla M, Crimi N and Raghu G: Idiopathic pulmonary fibrosis: A disease with similarities and links to cancer biology. Eur Respir J. 35:496–504. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Raghu G, Collard HR, Egan JJ, Martinez FJ, Behr J, Brown KK, Colby TV, Cordier JF, Flaherty KR, Lasky JA, et al: ATS/ERS/JRS/ALAT Committee on Idiopathic Pulmonary Fibrosis: An official ATS/ERS/JRS/ALAT statement: idiopathic pulmonary fibrosis: evidence-based guidelines for diagnosis and management. Am J Respir Crit Care Med. 183:788–824. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Turgut NH, Kara H, Elagoz S, Deveci K, Gungor H and Arslanbas E: The Protective effect of naringin against bleomycin-induced pulmonary fibrosis in Wistar rats. Pulm Med. 2016:76013932016. View Article : Google Scholar : PubMed/NCBI

8 

Moore BB and Hogaboam CM: Murine models of pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 294:L152–L160. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Bonnans C, Chou J and Werb Z: Remodelling the extracellular matrix in development and disease. Nat Rev Mol Cell Biol. 15:786–801. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Zhang Z, Jian X, Zhang W, Wang J and Zhou Q: Using bosentan to treat paraquat poisoning-induced acute lung injury in rats. PLoS One. 8:e759432013. View Article : Google Scholar : PubMed/NCBI

11 

Koo HS, Kim KC and Hong YM: Gene expressions of nitric oxide synthase and matrix metalloproteinase-2 in monocrotaline-induced pulmonary hypertension in rats after bosentan treatment. Korean Circ J. 41:83–90. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Giannelli G, Iannone F, Marinosci F, Lapadula G and Antonaci S: The effect of bosentan on matrix metalloproteinase-9 levels in patients with systemic sclerosis-induced pulmonary hypertension. Curr Med Res Opin. 21:327–332. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Wang HD, Yamaya M, Okinaga S, Jia YX, Kamanaka M, Takahashi H, Guo LY, Ohrui T and Sasaki H: Bilirubin ameliorates bleomycin-induced pulmonary fibrosis in rats. Am J Respir Crit Care Med. 165:406–411. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Bancroft JD and Gamble M: Connective tissue stainsTheory and practice of histological techniques. 6th. Elsevier Health Sciences; Churchill Livingstone: pp. 1502008

15 

Szapiel SV, Elson NA, Fulmer JD, Hunninghake GW and Crystal RG: Bleomycin-induced interstitial pulmonary disease in the nude, athymic mouse. Am Rev Respir Dis. 120:893–899. 1979.PubMed/NCBI

16 

Sleijfer S: Bleomycin-induced pneumonitis. Chest. 120:617–624. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Zhou XM, Zhang GC, Li JX and Hou J: Inhibitory effects of Hu-qi-yin on the bleomycin-induced pulmonary fibrosis in rats. J Ethnopharmacol. 111:255–264. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Araz O, Demirci E, Ucar E Yilmazel, Calik M, Pulur D, Karaman A, Yayla M, Altun E, Halici Z and Akgun M: Comparison of reducing effect on lung injury of dexamethasone and bosentan in acute lung injury: An experimental study. Multidiscip Respir Med. 8:74–81. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Watson WH, Ritzenthaler JD and Roman J: Lung extracellular matrix and redox regulation. Redox Biol. 8:305–315. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Teixeira KC, Soares FS, Rocha LG, Silveira PC, Silva LA, Valença SS, Dal Pizzol F, Streck EL and Pinho RA: Attenuation of bleomycin-induced lung injury and oxidative stress by N-acetylcysteine plus deferoxamine. Pulm Pharmacol Ther. 21:309–316. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Liang X, Tian Q, Wei Z, Liu F, Chen J, Zhao Y, Qu P, Huang X, Zhou X, Liu N, et al: Effect of Feining on bleomycin-induced pulmonary injuries in rats. J Ethnopharmacol. 134:971–976. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Arafa HM, AbdelWahab MH, ElShafeey MF, Badary OA and Hamada FM: Anti-fibrotic effect of meloxicam in a murine lung fibrosis model. Eur J Pharmacol. 564:181–189. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Yoon JS, Chae MK, Jang SY, Lee SY and Lee EJ: Antifibrotic effects of quercetin in primary orbital fibroblasts and orbital fat tissue cultures of Graves' orbitopathy. Invest Ophthalmol Vis Sci. 53:5921–5929. 2012. View Article : Google Scholar : PubMed/NCBI

24 

King TE Jr, Pardo A and Selman M: Idiopathic pulmonary fibrosis. Lancet. 378:1949–1961. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Pardo A, Cabrera S, Maldonado M and Selman M: Role of matrix metalloproteinases in the pathogenesis of idiopathic pulmonary fibrosis. Respir Res. 17:232016. View Article : Google Scholar : PubMed/NCBI

26 

Pardo A and Selman M: Matrix metalloproteases in aberrant fibrotic tissue remodeling. Proc Am Thorac Soc. 3:383–388. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Selman M, Ruiz V, Cabrera S, Segura L, Ramírez R, Barrios R and Pardo A: TIMP-1, −2, −3, and −4 in idiopathic pulmonary fibrosis. A prevailing nondegradative lung microenvironment? Am J Physiol Lung Cell Mol Physiol. 279:L562–L574. 2000.PubMed/NCBI

28 

Ramos C, Montaño M, García-Alvarez J, Ruiz V, Uhal BD, Selman M and Pardo A: Fibroblasts from idiopathic pulmonary fibrosis and normal lungs differ in growth rate, apoptosis, and tissue inhibitor of metalloproteinases expression. Am J Respir Cell Mol Biol. 24:591–598. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Zuo F, Kaminski N, Eugui E, Allard J, Yakhini Z, BenDor A, Lollini L, Morris D, Kim Y, DeLustro B, et al: Gene expression analysis reveals matrilysin as a key regulator of pulmonary fibrosis in mice and humans. Proc Natl Acad Sci USA. 99:6292–6297. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zuo WL, Zhao JM, Huang JX, Zhou W, Lei ZH, Huang YM, Huang YF and Li HG: Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis. Biomed Rep 6: 201-205, 2017.
APA
Zuo, W., Zhao, J., Huang, J., Zhou, W., Lei, Z., Huang, Y. ... Li, H. (2017). Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis. Biomedical Reports, 6, 201-205. https://doi.org/10.3892/br.2016.832
MLA
Zuo, W., Zhao, J., Huang, J., Zhou, W., Lei, Z., Huang, Y., Huang, Y., Li, H."Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis". Biomedical Reports 6.2 (2017): 201-205.
Chicago
Zuo, W., Zhao, J., Huang, J., Zhou, W., Lei, Z., Huang, Y., Huang, Y., Li, H."Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis". Biomedical Reports 6, no. 2 (2017): 201-205. https://doi.org/10.3892/br.2016.832
Copy and paste a formatted citation
x
Spandidos Publications style
Zuo WL, Zhao JM, Huang JX, Zhou W, Lei ZH, Huang YM, Huang YF and Li HG: Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis. Biomed Rep 6: 201-205, 2017.
APA
Zuo, W., Zhao, J., Huang, J., Zhou, W., Lei, Z., Huang, Y. ... Li, H. (2017). Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis. Biomedical Reports, 6, 201-205. https://doi.org/10.3892/br.2016.832
MLA
Zuo, W., Zhao, J., Huang, J., Zhou, W., Lei, Z., Huang, Y., Huang, Y., Li, H."Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis". Biomedical Reports 6.2 (2017): 201-205.
Chicago
Zuo, W., Zhao, J., Huang, J., Zhou, W., Lei, Z., Huang, Y., Huang, Y., Li, H."Effect of bosentan is correlated with MMP‑9/TIMP‑1 ratio in bleomycin‑induced pulmonary fibrosis". Biomedical Reports 6, no. 2 (2017): 201-205. https://doi.org/10.3892/br.2016.832
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team