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Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels

  • Authors:
    • Ying Zhou
    • Peng Yang
    • Aili Li
    • Xiaojun Ye
    • Shiyan Ren
    • Xianlun Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, China-Japan Friendship Hospital, Beijing 100029, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 188-194
    |
    Published online on: December 29, 2016
       https://doi.org/10.3892/br.2016.834
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Abstract

Prostaglandin E2 (PGE2) has been demonstrated to attenuate cardiac ischemia-reperfusion (I/R) injury. However, the underlying mechanism of PGE2 in cardiac I/R injury remains unknown. Upregulated expression levels of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) were reported in acute myocardial infarction (AMI), and were demonstrated to diminish I/R injury. In the current study the involvement of VEGF and eNOS in the myocardial protective effect of PGE2 were investigated in a catheter-based porcine model of AMI. Twenty-two Chinese miniature pigs were randomized into sham-surgery (n=6), control (n=8) and PGE2 (n=8) groups. PGE2 (1 µg/kg) was injected from 10 min prior to left anterior descending occlusion up to 1 h after reperfusion in the PGE2 group. Subsequently, the hemodynamic parameters were evaluated. Thioflavin-S and Evans Blue double staining were performed to evaluate the extent of the myocardial reperfusion area (RA) and no-reflow area (NRA). Immunohistochemical and western blot analysis were used to evaluate protein expression levels of VEGF and eNOS. Left ventricular (LV) systolic pressure significantly improved and LV end-diastolic pressure significantly decreased in the PGE2 group when compared with the control group 2 h after occlusion and 3 h after reperfusion (P<0.05, respectively). The RA and NRA were smaller in the PGE2 group than in the control group (P<0.05, respectively). Furthermore, PGE2 treatment increased the myocardial content of VEGF and eNOS when compared with the control group (P<0.05, respectively). Thus, the results of the present study demonstrate the cardio-protective mechanisms of PGE2, which may protect the heart from I/R injury via enhancement of VEGF and eNOS expression levels.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Yang P, Li A, Ye X, Ren S and Li X: Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels. Biomed Rep 6: 188-194, 2017.
APA
Zhou, Y., Yang, P., Li, A., Ye, X., Ren, S., & Li, X. (2017). Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels. Biomedical Reports, 6, 188-194. https://doi.org/10.3892/br.2016.834
MLA
Zhou, Y., Yang, P., Li, A., Ye, X., Ren, S., Li, X."Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels". Biomedical Reports 6.2 (2017): 188-194.
Chicago
Zhou, Y., Yang, P., Li, A., Ye, X., Ren, S., Li, X."Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels". Biomedical Reports 6, no. 2 (2017): 188-194. https://doi.org/10.3892/br.2016.834
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Yang P, Li A, Ye X, Ren S and Li X: Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels. Biomed Rep 6: 188-194, 2017.
APA
Zhou, Y., Yang, P., Li, A., Ye, X., Ren, S., & Li, X. (2017). Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels. Biomedical Reports, 6, 188-194. https://doi.org/10.3892/br.2016.834
MLA
Zhou, Y., Yang, P., Li, A., Ye, X., Ren, S., Li, X."Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels". Biomedical Reports 6.2 (2017): 188-194.
Chicago
Zhou, Y., Yang, P., Li, A., Ye, X., Ren, S., Li, X."Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels". Biomedical Reports 6, no. 2 (2017): 188-194. https://doi.org/10.3892/br.2016.834
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