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Article Open Access

Ex vivo regenerative effects of a spring water

  • Authors:
    • Giovanni Nicoletti
    • Marco Saler
    • Tommaso Pellegatta
    • Marco Mario Tresoldi
    • Viola Bonfanti
    • Alberto Malovini
    • Angela Faga
    • Federica Riva
  • View Affiliations / Copyright

    Affiliations: Plastic and Reconstructive Surgery, Department of Clinical‑Surgical Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy, Laboratory of Informatics and Systems Engineering for Clinical Research, Istituti Clinici Scientifici Maugeri, 27100 Pavia, Italy, Advanced Technologies for Regenerative Medicine and Inductive Surgery Research Center, University of Pavia, 27100 Pavia, Italy
    Copyright: © Nicoletti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 508-514
    |
    Published online on: October 18, 2017
       https://doi.org/10.3892/br.2017.1002
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Abstract

Previous experiments by our group have indicated the regenerative effects of a spring water (Comano), which was possibly associated with the native non‑pathogenic bacterial flora. The present study aimed to confirm these regenerative properties in a human ex vivo experimental model in the context of physiological wound healing. Human 6‑mm punch skin biopsies harvested during plastic surgery sessions were injured in their central portion to induce skin loss and were cultured in either conventional medium (controls) or medium powder reconstituted with filtered Comano spring water (treated samples). At 24, 48 and 72 h the specimens were observed following staining with hematoxylin and eosin, Picrosirius Red, orcein and anti‑proliferating cell nuclear antigen. Compared with the controls, the treated samples exhibited reduced overall cell infiltration, evidence of fibroblasts, stimulation of cell proliferation and collagen and elastic fiber regeneration. In the spring water, in addition to 12 resident non‑pathogenic bacterial strains exhibiting favorable metabolic activities, more unknown non‑pathogenic species are being identified by genomic analysis. In the present study, the efficacy of this ‘germ‑free’, filtered spring water in wound regeneration was indicated. Thus, the Comano spring water microbiota should be acknowledged for its regenerative properties.
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View References

1 

Belizário JE and Napolitano M: Human microbiomes and their roles in dysbiosis, common diseases, and novel therapeutic approaches. Front Microbiol. 6:10502015. View Article : Google Scholar : PubMed/NCBI

2 

Kanno E, Kawakami K, Ritsu M, Ishii K, Tanno H, Toriyabe S, Imai Y, Maruyama R and Tachi M: Wound healing in skin promoted by inoculation with Pseudomonas aeruginosa PAO1: The critical role of tumor necrosis factor-α secreted from infiltrating neutrophils. Wound Repair Regen. 19:608–621. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kostarnoy AV, Gancheva PG, Logunov DY, Verkhovskaya LV, Bobrov MA, Scheblyakov DV, Tukhvatulin AI, Filippova NE, Naroditsky BS and Gintsburg AL: Topical bacterial lipopolysaccharide application affects inflammatory response and promotes wound healing. J Interferon Cytokine Res. 33:514–522. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Faga A, Nicoletti G, Gregotti C, Finotti V, Nitto A and Gioglio L: Effects of thermal water on skin regeneration. Int J Mol Med. 29:732–740. 2012.PubMed/NCBI

5 

Nicoletti G, Saler M, Pellegatta T, Malovini A, Faga A, Scalise A and Riva F: Effects of a spring water on human skin fibroblasts in vitro cultures: Preliminary results. Acta Vulnol. 14:196–201. 2016.

6 

Aries MF, Fabre P, Duplan H, Hernandez Pigeon H, Galliano MF, Castex-Rizzi N, Bessou-Touya S and Nguyen T: I-modulia, an Aquaphilus dolomiae extract, stimulates innate immune response through Toll-like receptor activation. J Am Acad Dermatol. 70 Suppl 1:AB632014. View Article : Google Scholar

7 

Mahe YF, Perez MJ, Tacheau C, Fanchon C, Martin R, Rousset F and Seite S: A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway. Clin Cosmet Investig Dermatol. 6:191–196. 2013.PubMed/NCBI

8 

Nicoletti G, Corbella M, Jaber O, Marone P, Scevola D and Faga A: Non-pathogenic microflora of a spring water with regenerative properties. Biomed Rep. 3:758–762. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Jousson O: Culture-independent versus culture-dependent approaches for microbial community analysis of a thermal spring with therapeutic propertiesEMBL Symposium Heidelberg. New Approaches and Concepts in Microbiology; Germany: June 27–30–2017

10 

Nakamura M, Rikimaru T, Yano T, Moore KG, Pula PJ, Schofield BH and Dannenberg AM Jr: Full-thickness human skin explants for testing the toxicity of topically applied chemicals. J Invest Dermatol. 95:325–332. 1990. View Article : Google Scholar : PubMed/NCBI

11 

Mori M, Rossi S, Ferrari F, Bonferoni MC, Sandri G, Riva F, Tenci M, Del Fante C, Nicoletti G and Caramella C: Sponge-like dressings based on the association of chitosan and sericin for the treatment of chronic skin ulcers. II. Loading of the hemoderivative platelet lysate. J Pharm Sci. 105:1188–1195. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Fontana F, Mori M, Riva F, Mäkilä E, Liu D, Salonen J, Nicoletti G, Hirvonen J, Caramella C and Santos HA: Platelet lysate-modified porous silicon microparticles for enhanced cell proliferation in wound healing applications. ACS Appl Mater Interfaces. 8:988–996. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Junqueira LCU, Bignolas G and Brentani RR: Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem J. 11:447–455. 1979. View Article : Google Scholar : PubMed/NCBI

14 

Montes GS and Junqueira LC: The use of the Picrosirius-polarization method for the study of the biopathology of collagen. Mem Inst Oswaldo Cruz. 86 Suppl 3:1–11. 1991. View Article : Google Scholar : PubMed/NCBI

15 

Éditeur officiel du Québec: Regulation respecting bottled water. Food Products Act. Chapter P-29, s. 40. http://www.legisquebec.gouv.qc.ca/en/pdf/cr/S-2.1,%20R.%2013.pdfApril 24–2017

16 

Kompanets EV, Isaeva NM and Balakhnin IA: Bacteria of the genus Aeromonas and their role in aquaculture. Mikrobiol Zh. 54:89–99. 1992.(In Russian). PubMed/NCBI

17 

Erguven GO and Yildirim N: Efficiency of some soil bacteria for chemical oxygen demand reduction of synthetic chlorsulfuron solutions under agiated culture conditions. Cell Mol Biol (Noisy-le-grand). 62:92–96. 2016.PubMed/NCBI

18 

Han L, Zhao D and Li C: Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71. Braz J Microbiol. 46:433–441. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Cheriaa J, Mosrati R, Ladhari N and Bakhrouf A: Acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate. Pak J Biol Sci. 11:1588–1593. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Li C, Yang J, Wang X, Wang E, Li B, He R and Yuan H: Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24. Bioresour Technol. 182:18–25. 2015. View Article : Google Scholar : PubMed/NCBI

21 

El Amraoui B, El Amraoui M, Cohen N and Fassouane A: Antifungal and antibacterial activity of marine microorganisms. Ann Pharm Fr. 72:107–111. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Durán M, Faljoni-Alario A and Durán N: Chromobacterium violaceum and its important metabolites - review. Folia Microbiol (Praha). 55:535–547. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Hoshino T: Violacein and related tryptophan metabolites produced by Chromobacterium violaceum: Biosynthetic mechanism and pathway for construction of violacein core. Appl Microbiol Biotechnol. 91:1463–1475. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Rezzonico F, Smits TH, Montesinos E, Frey JE and Duffy B: Genotypic comparison of Pantoea agglomerans plant and clinical strains. BMC Microbiol. 9:2042009. View Article : Google Scholar : PubMed/NCBI

25 

Pellegatta T, Saler M, Bonfanti V, Nicoletti G and Faga A: Novel perspectives on the role of the human microbiota in regenerative medicine and surgery. Biomed Rep. 5:519–524. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Liang Z and Keeley A: Filtration recovery of extracellular DNA from environmental water samples. Environ Sci Technol. 47:9324–9331. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Safferling K, Sütterlin T, Westphal K, Ernst C, Breuhahn K, James M, Jäger D, Halama N and Grabe N: Wound healing revised: A novel reepithelialization mechanism revealed by in vitro and in silico models. J Cell Biol. 203:691–709. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Xu W, Jong Hong S, Jia S, Zhao Y, Galiano RD and Mustoe TA: Application of a partial-thickness human ex vivo skin culture model in cutaneous wound healing study. Lab Invest. 92:584–599. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Nicoletti G, Saler M, Pellegatta T, Tresoldi MM, Bonfanti V, Malovini A, Faga A and Riva F: Ex vivo regenerative effects of a spring water. Biomed Rep 7: 508-514, 2017.
APA
Nicoletti, G., Saler, M., Pellegatta, T., Tresoldi, M.M., Bonfanti, V., Malovini, A. ... Riva, F. (2017). Ex vivo regenerative effects of a spring water. Biomedical Reports, 7, 508-514. https://doi.org/10.3892/br.2017.1002
MLA
Nicoletti, G., Saler, M., Pellegatta, T., Tresoldi, M. M., Bonfanti, V., Malovini, A., Faga, A., Riva, F."Ex vivo regenerative effects of a spring water". Biomedical Reports 7.6 (2017): 508-514.
Chicago
Nicoletti, G., Saler, M., Pellegatta, T., Tresoldi, M. M., Bonfanti, V., Malovini, A., Faga, A., Riva, F."Ex vivo regenerative effects of a spring water". Biomedical Reports 7, no. 6 (2017): 508-514. https://doi.org/10.3892/br.2017.1002
Copy and paste a formatted citation
x
Spandidos Publications style
Nicoletti G, Saler M, Pellegatta T, Tresoldi MM, Bonfanti V, Malovini A, Faga A and Riva F: Ex vivo regenerative effects of a spring water. Biomed Rep 7: 508-514, 2017.
APA
Nicoletti, G., Saler, M., Pellegatta, T., Tresoldi, M.M., Bonfanti, V., Malovini, A. ... Riva, F. (2017). Ex vivo regenerative effects of a spring water. Biomedical Reports, 7, 508-514. https://doi.org/10.3892/br.2017.1002
MLA
Nicoletti, G., Saler, M., Pellegatta, T., Tresoldi, M. M., Bonfanti, V., Malovini, A., Faga, A., Riva, F."Ex vivo regenerative effects of a spring water". Biomedical Reports 7.6 (2017): 508-514.
Chicago
Nicoletti, G., Saler, M., Pellegatta, T., Tresoldi, M. M., Bonfanti, V., Malovini, A., Faga, A., Riva, F."Ex vivo regenerative effects of a spring water". Biomedical Reports 7, no. 6 (2017): 508-514. https://doi.org/10.3892/br.2017.1002
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