Analysis of IL6‑protein complexes in chondrosarcoma

Galoian,Karina; Luo,Shihua; Patel,Parthik

doi:10.3892/br.2017.1016

January-2018 Volume 8 Issue 1

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January-2018 Volume 8 Issue 1

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Article

Analysis of IL6‑protein complexes in chondrosarcoma

Authors:
- Karina Galoian
- Shihua Luo
- Parthik Patel
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Affiliations: Miller School of Medicine, Department of Orthopedics, University of Miami, Miami, FL 33136, USA
Pages: 91-98
|
Published online on: November 10, 2017

https://doi.org/10.3892/br.2017.1016
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Abstract

Cytokines produced in the tumour microenvironment serve important roles in cancer pathogenesis or in the supression of disease progression. Metastatic chondrosarcoma is a cancer of the cartilage, and our group previously reported from a human ELISA assay that interleukin 6 (IL6) expression in JJ012 chondrosarcoma cells was 86‑fold lower than that in C28 chondrocytes, indicating its role as an anti‑inflammatory and anti‑tumorigenic factor. Additionally, to the best of our knowledge, the study was the first to demonstrate downregulation of IL6 in a human chondrosarcoma cell line. To fully elucidate the effect of this IL6 downregulation, it is important to identify protein complexes and components that bind IL6 and potentially affect its gene expression directly or indirectly. To investigate IL6‑protein interactions leading to these differences in IL6 expression, the current study performed a gel retardation electrophoretic mobility shift assay (EMSA), followed by 2D gel phoresis, in‑gel trypsin digestion and proteomic mass spectral analysis. The results indicated a presence of ubiquitination enzymes in C28 chondrocytes, while none were identified in JJ012 chondrosarcoma cells. While it seems counterintuitive, it may be that the absence of ubiquitination of certain factors leads to the downregulation of IL6 expression in human chondrosarcoma. Therefore, dysregulated ubiquitination may be among the possible mechanisms for the markedly reduced IL6 expression in chondrosarcoma.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis of IL6‑protein complexes in chondrosarcoma

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