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Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury

  • Authors:
    • Jianhua Gong
    • Junyi Wang
    • Yu Tian
    • Jing Zhang
    • Wenjin Liang
    • Zeming Li
    • Jidong Yu
    • Bo Tang
    • Songqing He
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery and Laboratory, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China, Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 525-531
    |
    Published online on: April 11, 2017
       https://doi.org/10.3892/br.2017.891
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Abstract

The aim of the present study was to investigate the association between tubulin folding cofactor B (TBCB) expression and ischemia‑reperfusion injury (IRI) in mice. A total of 48 C57BL/6 mice were randomly divided into a control group (Sham, n=6) and an ischemia‑reperfusion group (n=42). The ischemia‑reperfusion group was further divided into 6 subgroups as per different times after reperfusion (2, 4, 6, 8, 12 and 24 h), with 7 mice per subgroup. A hepatic IRI model was established in mice by clamping the hepatic hilum. Morphology, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin 6 (IL‑6) and tumor necrosis factor‑α (TNF‑α), and the expression level of TBCB were detected. Compared with the control group, the livers from the ischemia‑reperfusion group were significantly changed, particularly at 12 h following ischemia‑reperfusion, with obvious hepatic cell degeneration and necrosis. The ALT, AST, IL‑6 and TNF‑α levels in the sera of the mice in the hepatic ischemia‑reperfusion group were increased at all time points following ischemia‑reperfusion, and were the highest at 12 h, demonstrating statistically significant differences when compared with the control group (P<0.05). Furthermore, the expression levels of TBCB, TNF‑α and IL‑6 were significantly increased at all time-points following ischemia‑reperfusion, and were the most significant at 12 h. At 24 h following ischemia‑reperfusion, the expression levels had decreased. The present study indicated that TBCB expression is associated with TNF‑α and IL‑6 expression levels in mice with hepatic ischemia‑reperfusion, and may be key in the development of liver injury during ischemia‑reperfusion in mice.
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Copy and paste a formatted citation
Spandidos Publications style
Gong J, Wang J, Tian Y, Zhang J, Liang W, Li Z, Yu J, Tang B and He S: Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury. Biomed Rep 6: 525-531, 2017.
APA
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z. ... He, S. (2017). Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury. Biomedical Reports, 6, 525-531. https://doi.org/10.3892/br.2017.891
MLA
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z., Yu, J., Tang, B., He, S."Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury". Biomedical Reports 6.5 (2017): 525-531.
Chicago
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z., Yu, J., Tang, B., He, S."Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury". Biomedical Reports 6, no. 5 (2017): 525-531. https://doi.org/10.3892/br.2017.891
Copy and paste a formatted citation
x
Spandidos Publications style
Gong J, Wang J, Tian Y, Zhang J, Liang W, Li Z, Yu J, Tang B and He S: Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury. Biomed Rep 6: 525-531, 2017.
APA
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z. ... He, S. (2017). Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury. Biomedical Reports, 6, 525-531. https://doi.org/10.3892/br.2017.891
MLA
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z., Yu, J., Tang, B., He, S."Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury". Biomedical Reports 6.5 (2017): 525-531.
Chicago
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z., Yu, J., Tang, B., He, S."Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury". Biomedical Reports 6, no. 5 (2017): 525-531. https://doi.org/10.3892/br.2017.891
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