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Article

The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells

  • Authors:
    • Seyed Younes Hosseini
    • Kazem Baesi
    • Negar Azarpira
    • Ameneh Pakneiat
    • Seyedeh Akram Hosseini
  • View Affiliations / Copyright

    Affiliations: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71348‑45794, Iran, Hepatitis and AIDS Department, Pasteur institute of Iran, Tehran 13169‑43551, Iran, Transplant Research Center, Shiraz University of Medical Sciences, Shiraz 14336‑71348, Iran, Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz 71348‑45794, Iran
  • Pages: 671-674
    |
    Published online on: April 19, 2017
       https://doi.org/10.3892/br.2017.894
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Abstract

The role of the hepatitis B virus (HBV) endogenous pre-core protein in liver fibrosis is controversial. Whether the expression of the pre-core induces the activation of human stellate cells (HSCs) has not yet been reported. Plasmids expressing HBx, or pre‑core protein were transfected into LX‑2 cells. Subsequently, total RNA extracted and reverse transcription-quantitative polymerase chain reaction was performed to measure the fold change of collagen type I, α1 chain, α‑smooth muscle actin and TIMP metalloproteinase inhibitor‑1. Moreover, transforming growth factor (TGF)‑β in the supernatant of HSCs was evaluated by ELISA assay. In addition, a MTT assay was performed to test the cytotoxicity of the endogenous expression in LX‑2 cells. None of the plasmids exhibited cytotoxic nor significant proliferative effects on LX-2 cells by MTT assessment. The gene expression analysis of fibrotic genes in LX‑2 cells demonstrated that the pre‑core protein presented no significant (P>0.05) fibrotic impact when compared to the empty control plasmid and HBx. The data from the TGF-β ELISA was consistent with the mRNA expression as detected with the control plasmid (P>0.05). The endogenous expression of the HBV pre‑core exhibited no fibrotic impression in HSCs when compared to HBx.
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1 

Gutierrez-Reyes G, Gutierrez-Ruiz MC and Kershenobich D: Liver fibrosis and chronic viral hepatitis. Arch Med Res. 38:644–651. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Mormone E, George J and Nieto N: Molecular pathogenesis of hepatic fibrosis and current therapeutic approaches. Chem Biol Interact. 193:225–231. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Khanizadeh S, Ravanshad M, Hosseini SY, Davoodian P, Zadeh AN, Sabahi F, Sarvari J, Khanlari Z and Hasani-Azad M: The possible role of NS3 protease activity of hepatitis C virus on fibrogenesis and miR-122 expression in hepatic stellate cells. Acta Virol. 60:242–248. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Liu X, Zhu ST, You H, Cong M, Liu TH, Wang BE and Jia JD: Hepatitis B virus infects hepatic stellate cells and affects their proliferation and expression of collagen type I. Chin Med J (Engl). 122:1455–1461. 2009.PubMed/NCBI

5 

Bataller R, Paik YH, Lindquist JN, Lemasters JJ and Brenner DA: Hepatitis C virus core and nonstructural proteins induce fibrogenic effects in hepatic stellate cells. Gastroenterology. 126:529–540. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Coenen M, Nischalke HD, Krämer B, Langhans B, Glässner A, Schulte D, Körner C, Sauerbruch T, Nattermann J and Spengler U: Hepatitis C virus core protein induces fibrogenic actions of hepatic stellate cells via toll-like receptor 2. Lab Invest. 91:1375–1382. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Shin JY, Hur W, Wang JS, Jang JW, Kim CW, Bae SH, Jang SK, Yang SH, Sung YC, Kwon OJ, et al: HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-beta1. Exp Mol Med. 37:138–145. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Wu CF, Lin YL and Huang YT: Hepatitis C virus core protein stimulates fibrogenesis in hepatic stellate cells involving the obese receptor. J Cell Biochem. 2012.

9 

Roseman AM, Berriman JA, Wynne SA, Butler PJ and Crowther RA: A structural model for maturation of the hepatitis B virus core. Proc Natl Acad Sci USA. 102:pp. 15821–15826. 2005; View Article : Google Scholar : PubMed/NCBI

10 

Lee BO, Tucker A, Frelin L, Sallberg M, Jones J, Peters C, Hughes J, Whitacre D, Darsow B, Peterson DL and Milich DR: Interaction of the hepatitis B core antigen and the innate immune system. J Immunol. 182:6670–6681. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Manigold T, Böcker U, Chen J, Gundt J, Traber P, Singer MV and Rossol S: Hepatitis B core antigen is a potent inductor of interleukin-18 in peripheral blood mononuclear cells of healthy controls and patients with hepatitis B infection. J Med Virol. 71:31–40. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Vanlandschoot P, van Houtte F, Serruys B and Leroux-Roels G: The arginine-rich carboxy-terminal domain of the hepatitis B virus core protein mediates attachment of nucleocapsids to cell-surface-expressed heparan sulfate. J Gen Virol. 86:75–84. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Mohamadkhani A, Jazii FR, Poustchi H, Nouraein O, Abbasi S, Sotoudeh M and Montazeri G: The role of mutations in core protein of hepatitis B virus in liver fibrosis. Virol J. 6:2092009. View Article : Google Scholar : PubMed/NCBI

14 

Zan Y, Zhang Y and Tien P: Hepatitis B virus e antigen induces activation of rat hepatic stellate cells. Biochem Biophys Res Commun. 435:391–396. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Xu L, Hui AY, Albanis E, Arthur MJ, O'Byrne SM, Blaner WS, Mukherjee P, Friedman SL and Eng FJ: Human hepatic stellate cell lines, LX-1 and LX-2: New tools for analysis of hepatic fibrosis. Gut. 54:142–151. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Hosseini SY, Kalantar K, Shahin K, Ghayour M, Rajabibazl M, Fattahi MR, et al: Comparison of the In Vitro Antifibrogenic Effects of Silymarin, Silybin A and 18α-Glycyrrhizin on Activated Hepatic Stellate Cells. Jundishapur J Nat Pharm Prod Inpress. e402852017.

17 

Khanizadeh S, Ravanshad M, Hosseini S, Davoodian P, Zadeh A Nejati and Sarvari J: Blocking of SMAD4 expression by shRNA effectively inhibits fibrogenesis of human hepatic stellate cells. Gastroenterol Hepatol Bed Bench. 8:262–269. 2015.PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Guo XL, Liang B, Wang XW, Fan FG, Jin J, Lan R, et al: Glycyrrhizic acid attenuates CCl4-induced hepatocyte apoptosis in rats via a p53-mediated pathway. World journal of gastroenterology. World J Gastroenterol. 19:37812013. View Article : Google Scholar : PubMed/NCBI

20 

Ye L, Yu H, Li C, Hirsch ML, Zhang L, Samulski RJ, Li W and Liu Z: Adeno-Associated Virus Vector Mediated Delivery of the HBV Genome Induces Chronic Hepatitis B Virus Infection and Liver Fibrosis in Mice. PLoS One. 10:e01300522015. View Article : Google Scholar : PubMed/NCBI

21 

Reeves HL and Friedman SL: Activation of hepatic stellate cells - a key issue in liver fibrosis. Front Biosci. 7:d808–d826. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Kisseleva T and Brenner DA: Hepatic stellate cells and the reversal of fibrosis. J Gastroenterol Hepatol. 21 Suppl 3:S84–S87. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Lin J, Wu JF, Zhang Q, Zhang HW and Cao GW: Virus-related liver cirrhosis: Molecular basis and therapeutic options. World J Gastroenterol. 20:6457–6469. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Martín-Vílchez S, Sanz-Cameno P, Rodríguez-Muñoz Y, Majano PL, Molina-Jiménez F, López-Cabrera M, Moreno-Otero R and Lara-Pezzi E: The hepatitis B virus X protein induces paracrine activation of human hepatic stellate cells. Hepatology. 47:1872–1883. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Guo GH, Tan DM, Zhu PA and Liu F: Hepatitis B virus X protein promotes proliferation and upregulates TGF-beta1 and CTGF in human hepatic stellate cell line, LX-2. Hepatobiliary Pancreat Dis Int. 8:59–64. 2009.PubMed/NCBI

26 

Chen HY, Chen ZX, Huang RF, Lin N and Wang XZ: Hepatitis B virus X protein activates human hepatic stellate cells through upregulating TGFbeta1. Genetics and molecular research. GMR. 13:8645–8656. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Wu JF, Ni YH, Chen HL, Hsu HY and Chang MH: The impact of hepatitis B virus precore/core gene carboxyl terminal mutations on viral biosynthesis and the host immune response. J Infect Dis. 209:1374–1381. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hosseini SY, Baesi K, Azarpira N, Pakneiat A and Hosseini SA: The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells. Biomed Rep 6: 671-674, 2017.
APA
Hosseini, S.Y., Baesi, K., Azarpira, N., Pakneiat, A., & Hosseini, S.A. (2017). The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells. Biomedical Reports, 6, 671-674. https://doi.org/10.3892/br.2017.894
MLA
Hosseini, S. Y., Baesi, K., Azarpira, N., Pakneiat, A., Hosseini, S. A."The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells". Biomedical Reports 6.6 (2017): 671-674.
Chicago
Hosseini, S. Y., Baesi, K., Azarpira, N., Pakneiat, A., Hosseini, S. A."The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells". Biomedical Reports 6, no. 6 (2017): 671-674. https://doi.org/10.3892/br.2017.894
Copy and paste a formatted citation
x
Spandidos Publications style
Hosseini SY, Baesi K, Azarpira N, Pakneiat A and Hosseini SA: The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells. Biomed Rep 6: 671-674, 2017.
APA
Hosseini, S.Y., Baesi, K., Azarpira, N., Pakneiat, A., & Hosseini, S.A. (2017). The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells. Biomedical Reports, 6, 671-674. https://doi.org/10.3892/br.2017.894
MLA
Hosseini, S. Y., Baesi, K., Azarpira, N., Pakneiat, A., Hosseini, S. A."The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells". Biomedical Reports 6.6 (2017): 671-674.
Chicago
Hosseini, S. Y., Baesi, K., Azarpira, N., Pakneiat, A., Hosseini, S. A."The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells". Biomedical Reports 6, no. 6 (2017): 671-674. https://doi.org/10.3892/br.2017.894
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