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Article

Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages

  • Authors:
    • Ba Reum Kim
    • Young‑Chang Cho
    • Hien Thi Thu Le
    • Huong Lan Vuong
    • Sewoong Lee
    • Sayeon Cho
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung‑Ang University, Seoul 06974, Republic of Korea
  • Pages: 691-697
    |
    Published online on: April 19, 2017
       https://doi.org/10.3892/br.2017.895
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Abstract

The rhizome of Anemarrhena asphodeloides Bunge (A. asphodeloides) has been used as a traditional East Asian medicine for the treatment of various types of inflammatory disease. However, to the best of our knowledge, there have been no systemic studies regarding the molecular mechanisms of action of the A. asphodeloides rhizome anti‑inflammatory effects. The aim of the present study was to elucidate the anti‑inflammatory effects and underlying mechanism of action of ethanol extracts of the rhizome of A. asphodeloides (EAA) in murine macrophages. Non‑cytotoxic concentrations of EAA (10-100 µg/ml) significantly decreased the production of NO and interleukin (IL)‑6 in lipopolysaccharide (LPS)-stimulated macrophages, while the production of tumor necrosis factor‑α was not regulated by EAA. EAA‑mediated reduction of nitric oxide (NO) was due to reduced expression levels of inducible NO synthase (iNOS). Furthermore, protein expression levels of LPS‑induced cyclooxygenase‑2, another inflammatory enzyme, were alleviated in the presence of EAA. EAA-mediated reduction of those proinflammatory mediators was due to inhibition of nuclear factor‑κB (NF‑κB) and activator protein 1 transcriptional activities followed by the stabilization of inhibitor of κ Bα and inhibition of p38, respectively. These results indicate that EAA suppresses LPS‑induced inflammatory responses by negatively regulating p38 and NF‑κB, indicating that EAA is a candidate treatment for alleviating inflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Kim BR, Cho YC, Le HT, Vuong HL, Lee S and Cho S: Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages. Biomed Rep 6: 691-697, 2017.
APA
Kim, B.R., Cho, Y., Le, H.T., Vuong, H.L., Lee, S., & Cho, S. (2017). Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages. Biomedical Reports, 6, 691-697. https://doi.org/10.3892/br.2017.895
MLA
Kim, B. R., Cho, Y., Le, H. T., Vuong, H. L., Lee, S., Cho, S."Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages". Biomedical Reports 6.6 (2017): 691-697.
Chicago
Kim, B. R., Cho, Y., Le, H. T., Vuong, H. L., Lee, S., Cho, S."Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages". Biomedical Reports 6, no. 6 (2017): 691-697. https://doi.org/10.3892/br.2017.895
Copy and paste a formatted citation
x
Spandidos Publications style
Kim BR, Cho YC, Le HT, Vuong HL, Lee S and Cho S: Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages. Biomed Rep 6: 691-697, 2017.
APA
Kim, B.R., Cho, Y., Le, H.T., Vuong, H.L., Lee, S., & Cho, S. (2017). Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages. Biomedical Reports, 6, 691-697. https://doi.org/10.3892/br.2017.895
MLA
Kim, B. R., Cho, Y., Le, H. T., Vuong, H. L., Lee, S., Cho, S."Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages". Biomedical Reports 6.6 (2017): 691-697.
Chicago
Kim, B. R., Cho, Y., Le, H. T., Vuong, H. L., Lee, S., Cho, S."Suppression of inflammation by the rhizome of Anemarrhena asphodeloides via regulation of nuclear factor-κB and p38 signal transduction pathways in macrophages". Biomedical Reports 6, no. 6 (2017): 691-697. https://doi.org/10.3892/br.2017.895
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