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Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human

  • Authors:
    • Jing Tang
    • Qiong Wu
    • Yi Li
    • Xiujuan Wu
    • Yujia Wang
    • Lihua Zhu
    • Yujun Shi
    • Hong Bu
    • Ji Bao
    • Mingjun Xie
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Medicine, Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China, Department of General Surgery, Yibin City First People's Hospital, Yibin, Sichuan 644000, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 627-632
    |
    Published online on: May 3, 2017
       https://doi.org/10.3892/br.2017.905
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Abstract

Genetic constructs with promoters fused to reporter genes for simultaneous monitoring of cellular events have been the focus of attention in recent years. Adenoviral vectors, which have distinctive characteristics, have been used to monitor the differentiation of stem cells in vitro. In the present study, a modified adenoviral vector was constructed, containing a mouse, rat, and human general albumin promoter sequence fused to a ZsGreen reporter gene, and evaluated its efficiency in different cell types. Two hepatocyte cell lines (Hepa1‑6 and HepG2), rat primary hepatocytes, rat bone marrow mesenchymal stem cells (BM‑MSCs) and rat BM‑MSCs‑derived hepatocyte‑like cells were transduced with this vector, and the transfection efficiency and functional capabilities of the promoter were evaluated by fluorescent microscopy. The results demonstrated efficient expression of ZsGreen in Hepa1‑6 cells, HepG2 cells, rat primary hepatocytes, and rat BM‑MSCs‑derived hepatocyte‑like cells, but not in rat BM‑MSCs. In conclusion, the current study demonstrates a simple, high‑efficiency, general tool for real‑time monitoring of the differentiation status of hepatocytes from stem cells in mice, rats, and humans. This tool may be useful for evaluating different protocols to generate functional hepatocytes from stem cells in multiple species.
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Copy and paste a formatted citation
Spandidos Publications style
Tang J, Wu Q, Li Y, Wu X, Wang Y, Zhu L, Shi Y, Bu H, Bao J, Xie M, Xie M, et al: Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomed Rep 6: 627-632, 2017.
APA
Tang, J., Wu, Q., Li, Y., Wu, X., Wang, Y., Zhu, L. ... Xie, M. (2017). Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomedical Reports, 6, 627-632. https://doi.org/10.3892/br.2017.905
MLA
Tang, J., Wu, Q., Li, Y., Wu, X., Wang, Y., Zhu, L., Shi, Y., Bu, H., Bao, J., Xie, M."Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human". Biomedical Reports 6.6 (2017): 627-632.
Chicago
Tang, J., Wu, Q., Li, Y., Wu, X., Wang, Y., Zhu, L., Shi, Y., Bu, H., Bao, J., Xie, M."Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human". Biomedical Reports 6, no. 6 (2017): 627-632. https://doi.org/10.3892/br.2017.905
Copy and paste a formatted citation
x
Spandidos Publications style
Tang J, Wu Q, Li Y, Wu X, Wang Y, Zhu L, Shi Y, Bu H, Bao J, Xie M, Xie M, et al: Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomed Rep 6: 627-632, 2017.
APA
Tang, J., Wu, Q., Li, Y., Wu, X., Wang, Y., Zhu, L. ... Xie, M. (2017). Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomedical Reports, 6, 627-632. https://doi.org/10.3892/br.2017.905
MLA
Tang, J., Wu, Q., Li, Y., Wu, X., Wang, Y., Zhu, L., Shi, Y., Bu, H., Bao, J., Xie, M."Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human". Biomedical Reports 6.6 (2017): 627-632.
Chicago
Tang, J., Wu, Q., Li, Y., Wu, X., Wang, Y., Zhu, L., Shi, Y., Bu, H., Bao, J., Xie, M."Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human". Biomedical Reports 6, no. 6 (2017): 627-632. https://doi.org/10.3892/br.2017.905
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