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Article

Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases

  • Authors:
    • Mahyar Nourian
    • Vahid Chaleshi
    • Leila Pishkar
    • Pedram Azimzadeh
    • Shaghayegh Baradaran Ghavami
    • Hedieh Balaii
    • Samaneh Alinaghi
    • Shabnam Shahrokh
    • Hamid Asadzadeh Aghdaei
    • Mohammad Reza Zali
  • View Affiliations / Copyright

    Affiliations: Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran, Young Researchers and Elite Club, Islamshahr Branch, Islamic Azad University, Islamshahr 3314767653, Iran, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran
  • Pages: 698-702
    |
    Published online on: May 9, 2017
       https://doi.org/10.3892/br.2017.908
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Abstract

Crohn's disease (CD) and ulcerative colitis (UC) are types of chronic inflammatory bowel disease (IBD) of which the actual causes remain unknown. Emerging data indicate that alterations in cytokine synthesis may be involved in IBD pathogenesis. The aim of the present study was to determine whether the tumor necrosis factor (TNF)‑α mRNA expression level and rs1799964 polymorphism are the genetic susceptibility component of IBD development. The TNF‑α mRNA expression level of peripheral blood mononuclear cells (PBMCs) was measured using comparative reverse‑transcription quantitative polymerase chain reaction (PCR). Genomic DNA from 201 individuals (CD: n=15; UC: n=86; control subjects: n=100) was analyzed for the presence of the TNF‑α‑1031 polymorphism by PCR‑restriction fragment length polymorphism. An increased TNF‑α mRNA expression level was additionally observed in the CC genotype of the ‑1031 TNF‑α gene polymorphism compared with the TC and TT genotypes (P<0.05). Furthermore, the present results revealed that there was no significant difference in the genotype/allele frequencies of the ‑1031 TNF‑α gene polymorphism in Iranian IBD patients. By comparison, the TNF‑α mRNA expression level was evaluated in patients with a history of taking medications and demonstrated a significant association in the group that received the 5‑ASA + Pred + AZA,5. 5‑ASA + Pred + AZA + IFX when compared with the other groups (P<0.05). Thus, these results support the hypothesis that overexpression of the TNF‑α gene, which correlated with the CC genotype, may represent a genetic risk factor for Iranian IBD.
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Copy and paste a formatted citation
Spandidos Publications style
Nourian M, Chaleshi V, Pishkar L, Azimzadeh P, Baradaran Ghavami S, Balaii H, Alinaghi S, Shahrokh S, Asadzadeh Aghdaei H, Zali MR, Zali MR, et al: Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases. Biomed Rep 6: 698-702, 2017.
APA
Nourian, M., Chaleshi, V., Pishkar, L., Azimzadeh, P., Baradaran Ghavami, S., Balaii, H. ... Zali, M.R. (2017). Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases. Biomedical Reports, 6, 698-702. https://doi.org/10.3892/br.2017.908
MLA
Nourian, M., Chaleshi, V., Pishkar, L., Azimzadeh, P., Baradaran Ghavami, S., Balaii, H., Alinaghi, S., Shahrokh, S., Asadzadeh Aghdaei, H., Zali, M. R."Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases". Biomedical Reports 6.6 (2017): 698-702.
Chicago
Nourian, M., Chaleshi, V., Pishkar, L., Azimzadeh, P., Baradaran Ghavami, S., Balaii, H., Alinaghi, S., Shahrokh, S., Asadzadeh Aghdaei, H., Zali, M. R."Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases". Biomedical Reports 6, no. 6 (2017): 698-702. https://doi.org/10.3892/br.2017.908
Copy and paste a formatted citation
x
Spandidos Publications style
Nourian M, Chaleshi V, Pishkar L, Azimzadeh P, Baradaran Ghavami S, Balaii H, Alinaghi S, Shahrokh S, Asadzadeh Aghdaei H, Zali MR, Zali MR, et al: Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases. Biomed Rep 6: 698-702, 2017.
APA
Nourian, M., Chaleshi, V., Pishkar, L., Azimzadeh, P., Baradaran Ghavami, S., Balaii, H. ... Zali, M.R. (2017). Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases. Biomedical Reports, 6, 698-702. https://doi.org/10.3892/br.2017.908
MLA
Nourian, M., Chaleshi, V., Pishkar, L., Azimzadeh, P., Baradaran Ghavami, S., Balaii, H., Alinaghi, S., Shahrokh, S., Asadzadeh Aghdaei, H., Zali, M. R."Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases". Biomedical Reports 6.6 (2017): 698-702.
Chicago
Nourian, M., Chaleshi, V., Pishkar, L., Azimzadeh, P., Baradaran Ghavami, S., Balaii, H., Alinaghi, S., Shahrokh, S., Asadzadeh Aghdaei, H., Zali, M. R."Evaluation of tumor necrosis factor (TNF)-α mRNA expression level and the rs1799964 polymorphism of the TNF-α gene in peripheral mononuclear cells of patients with inflammatory bowel diseases". Biomedical Reports 6, no. 6 (2017): 698-702. https://doi.org/10.3892/br.2017.908
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