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Article

Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model

  • Authors:
    • Airton Pereira E Silva
    • Sylvia Maria Nicolau Campos
    • Isabelle Mazza Guimarães
    • Gerlinde Agate Platais Brasil Teixeira
  • View Affiliations / Copyright

    Affiliations: Gastrointestinal Immunology Group, Department of Immunobiology, Institute of Biology, Federal Fluminense University, Niterói, Rio de Janeiro 24020‑150, Brazil
  • Pages: 247-250
    |
    Published online on: July 21, 2017
       https://doi.org/10.3892/br.2017.950
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Abstract

Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niterói, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research. Thus, a mouse gut inflammation model developed by our research group was used in the present study. Analysis was performed using optical microscopy and digital imaging using the APERIO scanning system and the ImageScope® software. Intestinal epithelial cells (IECs), intra epithelial leucocytes (IEL), and villi number and area were evaluated. No significant differences were observed in villi number, IEC and IEL; however, the villi area was significantly smaller when measured using the computer. Thus, the present study indicates that virtual microscopy is a trustworthy method for research purposes.
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1 

Boyce BF: Whole slide imaging: Uses and limitations for surgical pathology and teaching. Biotech Histochem. 90:321–330. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Bogner A, Jouneau PH, Thollet G, Basset D and Gauthier C: A history of scanning electron microscopy developments: Towards ‘wet-STEM’ imaging. Micron. 38:390–401. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Hawkes PW: The correction of electron lens aberrations. Ultramicroscopy. 156:A1–A64. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Banavar SR, Chippagiri P, Pandurangappa R, Annavajjula S and Rajashekaraiah PB: Image montaging for creating a virtual pathology slide: An innovative and economical tool to obtain a whole slide image. Anal Cell Pathol (Amst). 2016:90849092016.PubMed/NCBI

5 

Dee FR: Virtual microscopy in pathology education. Hum Pathol. 40:1112–1121. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Silage DA and Gil J: Digital image tiles: A method for the processing of large sections. J Microsc. 138:221–227. 1985. View Article : Google Scholar : PubMed/NCBI

7 

Westerkamp D and Gahm T: Non-distorted assemblage of the digital images of adjacent fields in histological sections. Anal Cell Pathol. 5:235–247. 1993.PubMed/NCBI

8 

Furness PN: The use of digital images in pathology. J Pathol. 183:253–263. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Arena ET, Rueden CT, Hiner MC, Wang S, Yuan M and Eliceiri KW: Quantitating the cell: Turning images into numbers with ImageJ. Wiley Interdiscip Rev Dev Biol. 2016.PubMed/NCBI

10 

Entenberg D, Rodriguez-Tirado C, Kato Y, Kitamura T, Pollard JW and Condeelis J: In vivo subcellular resolution optical imaging in the lung reveals early metastatic proliferation and motility. Intravital. 4:1–11. 2015. View Article : Google Scholar

11 

Indu M, Rathy R and Binu MP: ‘Slide less pathology’: Fairy tale or reality? J Oral Maxillofac Pathol. 20:284–288. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Pantanowitz L, Valenstein PN, Evans AJ, Kaplan KJ, Pfeifer JD, Wilbur DC, Collins LC and Colgan TJ: Review of the current state of whole slide imaging in pathology. J Pathol Inform. 2:362011. View Article : Google Scholar : PubMed/NCBI

13 

García-Rojo M, Sánchez J, de la Santa E, Durán E, Ruiz JL, Silva A, Rubio FJ, Rodríguez AM, Meléndez B, González L, et al: Automated image analysis in the study of lymphocyte subpopulation in eosinophilic oesophagitis. Diagn Pathol. 9:S72014. View Article : Google Scholar : PubMed/NCBI

14 

Higgins C: Applications and challenges of digital pathology and whole slide imaging. Biotech Histochem. 90:341–347. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Rocha R, Vassallo J, Soares F, Miller K and Gobbi H: Digital slides: Present status of a tool for consultation, teaching, and quality control in pathology. Pathol Res Pract. 205:735–741. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Hamilton PW, Wang Y and McCullough SJ: Virtual microscopy and digital pathology in training and education. APMIS. 120:305–315. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Camparo P, Egevad L, Algaba F, Berney DM, Boccon-Gibod L, Compérat E, Evans AJ, Grobholz R, Kristiansen G, Langner C, et al: Utility of whole slide imaging and virtual microscopy in prostate pathology. APMIS. 120:298–304. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Lundström C, Thorstenson S, Waltersson M, Persson A and Treanor D: Summary of 2(nd) Nordic symposium on digital pathology. J Pathol Inform. 6:52015. View Article : Google Scholar : PubMed/NCBI

19 

Teixeira G, Paschoal PO, de Oliveira VL, Pedruzzi MM, Campos SM, Andrade L and Nóbrega A: Diet selection in immunologically manipulated mice. Immunobiology. 213:1–12. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Yu W, Freeland DMH and Nadeau KC: Food allergy: Immune mechanisms, diagnosis and immunotherapy. Nat Rev Immunol. 16:751–765. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Dogan S, Astvatsatourov A, Deserno TM, Bock F, Shah-Hosseini K, Michels A and Mösges R: Objectifying the conjunctival provocation test: Photography-based rating and digital analysis. Int Arch Allergy Immunol. 163:59–68. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Bachert C, Borchard U, Wedi B, Klimek L, Rasp G, Riechelmann H, Schultze-Werninghaus G, Wahn U and Ring J: Allergic rhinoconjunctivitis. Guidelines of the DGAI in association with the DDG. J Dtsch Dermatol Ges. 4:264–275. 2006.(In German). View Article : Google Scholar : PubMed/NCBI

23 

Campos SM, de Oliveira VL, Lessa L, Vita M, Conceição M, Andrade LA and Teixeira GA: Maternal immunomodulation of the offspring's immunological system. Immunobiology. 219:813–821. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Paschoal PO, Campos SM, Pedruzzi MM, Garrido V, Bisso M, Antunes DM, Nobrega AF and Teixeira G: Food allergy/hypersensitivity: Antigenicity or timing? Immunobiology. 214:269–278. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Casuccio GS, Janocko PB, Lee RJ, Kelly JF, Dattner SL and Mgebroff JS: The use of computer controlled scanning electron microscopy in environmental studies. J Air Pollut Control Assoc. 33:937–943. 1983. View Article : Google Scholar

26 

Apotheker H and Jako GJ: A microscope for use in dentistry. J Microsurg. 3:7–10. 1981. View Article : Google Scholar : PubMed/NCBI

27 

Fisher RB and Parsons DS: The gradient of mucosal surface area in the small intestine of the rat. J Anat. 84:272–282. 1950.PubMed/NCBI

28 

Wood HO: The surface area of the intestinal mucosa in the rat and in the cat. J Anat. 78:103–105. 1944.PubMed/NCBI

29 

Marsh MN: Intestinal Manifestations of food hypersensitivity. 34. Raven Press; New York, NY: 1995

30 

Oberhuber G, Granditsch G and Vogelsang H: The histopathology of coeliac disease: Time for a standardized report scheme for pathologists. Eur J Gastroenterol Hepatol. 11:1185–1194. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Dickson BC, Streutker CJ and Chetty R: Coeliac disease: An update for pathologists. J Clin Pathol. 59:1008–1016. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pereira E Silva A, Nicolau Campos SM, Guimarães IM and Agate Platais Brasil Teixeira G: Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model. Biomed Rep 7: 247-250, 2017.
APA
Pereira E Silva, A., Nicolau Campos, S.M., Guimarães, I.M., & Agate Platais Brasil Teixeira, G. (2017). Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model. Biomedical Reports, 7, 247-250. https://doi.org/10.3892/br.2017.950
MLA
Pereira E Silva, A., Nicolau Campos, S. M., Guimarães, I. M., Agate Platais Brasil Teixeira, G."Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model". Biomedical Reports 7.3 (2017): 247-250.
Chicago
Pereira E Silva, A., Nicolau Campos, S. M., Guimarães, I. M., Agate Platais Brasil Teixeira, G."Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model". Biomedical Reports 7, no. 3 (2017): 247-250. https://doi.org/10.3892/br.2017.950
Copy and paste a formatted citation
x
Spandidos Publications style
Pereira E Silva A, Nicolau Campos SM, Guimarães IM and Agate Platais Brasil Teixeira G: Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model. Biomed Rep 7: 247-250, 2017.
APA
Pereira E Silva, A., Nicolau Campos, S.M., Guimarães, I.M., & Agate Platais Brasil Teixeira, G. (2017). Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model. Biomedical Reports, 7, 247-250. https://doi.org/10.3892/br.2017.950
MLA
Pereira E Silva, A., Nicolau Campos, S. M., Guimarães, I. M., Agate Platais Brasil Teixeira, G."Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model". Biomedical Reports 7.3 (2017): 247-250.
Chicago
Pereira E Silva, A., Nicolau Campos, S. M., Guimarães, I. M., Agate Platais Brasil Teixeira, G."Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model". Biomedical Reports 7, no. 3 (2017): 247-250. https://doi.org/10.3892/br.2017.950
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