Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
October-2017 Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2017 Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy

  • Authors:
    • Elizaveta Ershova
    • Vasilina Sergeeva
    • Maria Klimenko
    • Kristina Avetisova
    • Peter Klimenko
    • Edmund Kostyuk
    • Natalia Veiko
    • Roman Veiko
    • Vera Izevskaya
    • Sergey Kutsev
    • Svetlana Kostyuk
  • View Affiliations / Copyright

    Affiliations: Federal State Budgetary Institution ‘Research Centre For Medical Genetics’, Moscow 115478, Russia, Pediatric Faculty, Pirogov Russian National Research Medical University, Moscow 117997, Russia, Center of Family Planning and Reproduction, Moscow 117209, Russia
  • Pages: 319-324
    |
    Published online on: August 17, 2017
       https://doi.org/10.3892/br.2017.968
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The level of apoptosis is increased during pregnancy. Dying cells emit DNA that remains in blood circulation and is known as cell‑free DNA (cfDNA). The concentration of cfDNA can reflect the level of cell death. The present article is the result of studying cfDNA concentration and DNase I activity in the blood plasma of 40 non‑pregnant women (control), 40 healthy pregnant women (over 37 weeks) and 40 pregnant women with a diagnosis of intrauterine growth restriction (IUGR). In order to explain the obtained results, a program modeling the change of cfDNA concentration under the influence of different internal and external factors was written. It was reported that, despite the fact that the level of cell death is increased, cfDNA concentration in blood can be decreased due to activation of cfDNA elimination system. A significant increase of DNase I activity has been reported in cases of IUGR. Increase in DNase I activity over a certain threshold indicates presence of pathological processes in the organism. CfDNA circulating in blood cannot be a reliable marker of increased cell death during pregnancy. Thus, assessment of the level of cell death during pregnancy should be done by simultaneous analysis of cfDNA level and DNase I activity.
View Figures

Figure 1

Figure 2

View References

1 

Gahan PB and Stroun M: The Biology of Circulating Nucleic Acids in Plasma and Serum (CNAPS)Extracellular Nucleic Acids. Kikuchi Yo and Rykova EY: Springer; Berlin: pp. 167–189. 2010, View Article : Google Scholar

2 

Mittra I, Nair NK and Mishra PK: Nucleic acids in circulation: Are they harmful to the host? J Biosci. 37:301–312. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Rykova EY, Morozkin ES, Ponomaryova AA, Loseva EM, Zaporozhchenko IA, Cherdyntseva NV, Vlassov VV and Laktionov PP: Cell-free and cell-bound circulating nucleic acid complexes: Mechanisms of generation, concentration and content. Expert Opin Biol Ther. 12 Suppl 1:S141–S153. 2012. View Article : Google Scholar : PubMed/NCBI

4 

van der Vaart M and Pretorius PJ: Characterization of circulating DNA in healthy human plasma. Clin Chim Acta. 395:1862008. View Article : Google Scholar : PubMed/NCBI

5 

van der Vaart M and Pretorius PJ: Circulating DNA. Its origin and fluctuation. Ann N Y Acad Sci. 1137:18–26. 2008. View Article : Google Scholar : PubMed/NCBI

6 

van der Vaart M and Pretorius PJ: The origin of circulating free DNA. Clin Chem. 53:22152007. View Article : Google Scholar : PubMed/NCBI

7 

Gahan PB: Biology of circulating nucleic acids and possible roles in diagnosis and treatment in diabetes and cancer. Infect Disord Drug Targets. 12:360–370. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Gahan PB, Anker P and Stroun M: Metabolic DNA as the origin of spontaneously released DNA? Ann N Y Acad Sci. 1137:7–17. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Gahan PB and Stroun M: The virtosome-a novel cytosolic informative entity and intercellular messenger. Cell Biochem Funct. 28:529–538. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Lo YM, Corbetta N, Chamberlain PF, Rai V, Sargent IL, Redman CW and Wainscoat JS: Presence of fetal DNA in maternal plasma and serum. Lancet. 350:485–487. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Gahan PB: Circulating nucleic acids in plasma and serum: Applications in diagnostic techniques for noninvasive prenatal diagnosis. Int J Womens Health. 5:177–186. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Bianchi DW and Wilkins-Haug L: Integration of noninvasive DNA testing for aneuploidy into prenatal care: What has happened since the rubber met the road? Clin Chem. 60:78–87. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Sifakis S, Koukou Z and Spandidos DA: Cell-free fetal DNA and pregnancy-related complications (Review). Mol Med Rep. 11:2367–2372. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Hahn S, Rusterholz C, Hösli I and Lapaire O: Cell-free nucleic acids as potential markers for preeclampsia. Placenta. 32 Suppl:S17–S20. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Dugoff L, Barberio A, Whittaker PG, Schwartz N, Sehdev H and Bastek JA: Cell-free DNA fetal fraction and preterm birth. Am J Obstet Gynecol. 215:231.e1–7. 2016. View Article : Google Scholar

16 

Sekizawa A, Jimbo M, Saito H, Iwasaki M, Matsuoka R, Okai T and Farina A: Cell-free fetal DNA in the plasma of pregnant women with severe fetal growth restriction. Am J Obstet Gynecol. 188:480–484. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Gourvas V, Dalpa E, Konstantinidou A, Vrachnis N, Spandidos DA and Sifakis S: Angiogenic factors in placentas from pregnancies complicated by fetal growth restriction (Review). Mol Med Rep. 6:23–27. 2012.PubMed/NCBI

18 

Smid M, Galbiati S, Lojacono A, Valsecchi L, Platto C, Cavoretto P, Calza S, Ferrari A, Ferrari M and Cremonesi L: Correlation of fetal DNA levels in maternal plasma with Doppler status in pathological pregnancies. Prenat Diagn. 26:785–790. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Alberry MS, Maddocks DG, Hadi MA, Metawi H, Hunt LP, Abdel-Fattah SA, Avent ND and Soothill PW: Quantification of cell free fetal DNA in maternal plasma in normal pregnancies and in pregnancies with placental dysfunction. Am J Obstet Gynecol. 200:98.e1–98.e6. 2009. View Article : Google Scholar

20 

Al Nakib M, Desbrière R, Bonello N, Bretelle F, Boubli L, Gabert J and Levy-Mozziconacci A: Total and fetal cell-free DNA analysis in maternal blood as markers of placental insufficiency in intrauterine growth restriction. Fetal Diagn Ther. 26:24–28. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Veĭko NN, Bulycheva NV, Roginko OA, Veĭko RV, Ershova ES, Kozdoba OA, Kuz'min VA, Vinogradov AM, Iudin AA and Speranskiĭ AI: Ribosomal repeat in the cell free DNA as a marker for cell death. Biomed Khim. 54:78–93. 2008.(In Russian). PubMed/NCBI

22 

Korzeneva IB, Kostuyk SV, Ershova LS, Osipov AN, Zhuravleva VF, Pankratova GV, Porokhovnik LN and Veiko NN: Human circulating plasma DNA significantly decreases while lymphocyte DNA damage increases under chronic occupational exposure to low-dose gamma-neutron and tritium β-radiation. Mutat Res. 779:1–15. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Macanovic M and Lachmann PJ: Measurement of deoxyribonuclease I (DNase) in the serum and urine of systemic lupus erythematosus (SLE)-prone NZB/NZW mice by a new radial enzyme diffusion assay. Clin Exp Immunol. 108:220–226. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Gannushkina IV and Konorova IL: Cell-free plasmic DNA as a blood factor determining hemodynamics in health and in vascular pathology]. Patol Fiziol Eksp Ter. 2–10. 2008.(In Russian). PubMed/NCBI

25 

Speranskii AI, Kostyuk SV, Veiko NN and Kalashnikova EA: Enrichment of extracellular DNA from the cultivation medium of human peripheral blood mononuclears with genomic CpG rich fragments results in increased cell production of IL-6 and TNF-a via activation of the NF-kB signaling pathway. Biomed Khim. 62:331–340. 2016.(In Russian). View Article : Google Scholar : PubMed/NCBI

26 

Seredkina N, Zykova SN and Rekvig OP: Progression of murine lupus nephritis is linked to acquired renal Dnase1 deficiency and not to up-regulated apoptosis. Am J Pathol. 175:97–106. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Fenton K, Fismen S, Hedberg A, Seredkina N, Fenton C, Mortensen ES and Rekvig OP: Anti-dsDNA antibodies promote initiation, and acquired loss of renal Dnase1 promotes progression of lupus nephritis in autoimmune (NZBxNZW)F1 mice. PLoS One. 4:e84742009. View Article : Google Scholar : PubMed/NCBI

28 

Kolialexi A, Tsangaris GT, Antsaklis A, Tzortzatou F, Amentas C, Koratzis A and Mavrou A: Apoptosis in maternal peripheral blood during pregnancy. Fetal Diagn Ther. 16:32–37. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Kolialexi A, Tsangaris GT, Mavrou A, Antsaklis A, Tzortzatou F, Touliatou V and Metaxotou C: Use of annexin V antibody to identify apoptotic cells during pregnancy. Ann N Y Acad Sci. 945:145–150. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ershova E, Sergeeva V, Klimenko M, Avetisova K, Klimenko P, Kostyuk E, Veiko N, Veiko R, Izevskaya V, Kutsev S, Kutsev S, et al: Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy. Biomed Rep 7: 319-324, 2017.
APA
Ershova, E., Sergeeva, V., Klimenko, M., Avetisova, K., Klimenko, P., Kostyuk, E. ... Kostyuk, S. (2017). Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy. Biomedical Reports, 7, 319-324. https://doi.org/10.3892/br.2017.968
MLA
Ershova, E., Sergeeva, V., Klimenko, M., Avetisova, K., Klimenko, P., Kostyuk, E., Veiko, N., Veiko, R., Izevskaya, V., Kutsev, S., Kostyuk, S."Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy". Biomedical Reports 7.4 (2017): 319-324.
Chicago
Ershova, E., Sergeeva, V., Klimenko, M., Avetisova, K., Klimenko, P., Kostyuk, E., Veiko, N., Veiko, R., Izevskaya, V., Kutsev, S., Kostyuk, S."Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy". Biomedical Reports 7, no. 4 (2017): 319-324. https://doi.org/10.3892/br.2017.968
Copy and paste a formatted citation
x
Spandidos Publications style
Ershova E, Sergeeva V, Klimenko M, Avetisova K, Klimenko P, Kostyuk E, Veiko N, Veiko R, Izevskaya V, Kutsev S, Kutsev S, et al: Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy. Biomed Rep 7: 319-324, 2017.
APA
Ershova, E., Sergeeva, V., Klimenko, M., Avetisova, K., Klimenko, P., Kostyuk, E. ... Kostyuk, S. (2017). Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy. Biomedical Reports, 7, 319-324. https://doi.org/10.3892/br.2017.968
MLA
Ershova, E., Sergeeva, V., Klimenko, M., Avetisova, K., Klimenko, P., Kostyuk, E., Veiko, N., Veiko, R., Izevskaya, V., Kutsev, S., Kostyuk, S."Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy". Biomedical Reports 7.4 (2017): 319-324.
Chicago
Ershova, E., Sergeeva, V., Klimenko, M., Avetisova, K., Klimenko, P., Kostyuk, E., Veiko, N., Veiko, R., Izevskaya, V., Kutsev, S., Kostyuk, S."Circulating cell‑free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy". Biomedical Reports 7, no. 4 (2017): 319-324. https://doi.org/10.3892/br.2017.968
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team