Microsatellite analysis of genetic diversity in the Tupaia belangeri yaoshanensis

Su,Ao-Lei; Lan,Xiu-Wan; Huang,Ming-Bo; Nong,Wei; Li,Qingdi  Quentin; Leng,Jing

doi:10.3892/br.2017.969

October-2017 Volume 7 Issue 4

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October-2017 Volume 7 Issue 4

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Article

Microsatellite analysis of genetic diversity in the Tupaia belangeri yaoshanensis

Authors:
- Ao-Lei Su
- Xiu-Wan Lan
- Ming-Bo Huang
- Wei Nong
- Qingdi Quentin Li
- Jing Leng
View Affiliations / Copyright

Affiliations: Department of Microbiology and Immunology, Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models of Guangxi, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China, Department of Biochemistry and Molecule Biology, Guangxi Colleges and Universities Key Laboratory of Preclinical Medicine Research, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, GA 30310, USA, National Institutes of Health, Bethesda, MD 20892, USA
Pages: 349-352
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Published online on: August 18, 2017

https://doi.org/10.3892/br.2017.969
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Abstract

The Chinese tree shrew (Tupaia belangeri yaoshanensis) has long been proposed to serve as an animal model for studying human diseases. However, its overall genetic diversity and population structure remain largely unknown. In the present study, we investigated the genetic diversity of population microsatellite DNA in wild Tupaia belangeri yaoshanensis. Sixteen microsatellite loci were assessed in 76 wild Tupaia belangeri yaoshanensis. The target microsatellite DNA fragments were amplified from the peripheral blood DNA of the animals by polymerase chain reaction (PCR), and the PCR-amplified products were verified by DNA sequencing and used for the analysis of allele, effective allele, genetic heterozygosity, polymorphism and population structure. Our results showed that of the 16 microsatellite loci examined, 5 microsatellite loci were monomorphic and 11 microsatellite loci were polymorphic. We detected 61 alleles in the polymorphic loci and found 2-10 (with an average of 5.5455) alleles per locus. Our data also showed that the observed and expected heterozygosities ranged from 0.087 to 0.8947 and 0.1368 to 0.7892 with an average of 0.3968 and 0.4796, respectively. Taken together, the results revealed a considerably high heterozygosity and high genetic diversity at the molecular level in the population of wild Tupaia belangeri yaoshanensis. The identified markers from the present study may be useful for individual identification and parentage testing, as well as for the quantification of population heterogeneity in the Chinese tree shrew.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Microsatellite analysis of genetic diversity in the Tupaia belangeri yaoshanensis

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