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Article

Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells

  • Authors:
    • Chung-Lung Cho
    • Ya-Zhe Lee
    • Chao-Neng Tseng
    • Joshua Cho
    • Yuan-Bin Cheng
    • Kuo‑Wei Wang
    • Han-Jung Chen
    • Shean-Jaw Chiou
    • Chia-Hua Chou
    • Yi-Ren Hong
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, R.O.C., University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA, Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C., Department of Neurosurgery, E-Da Hospital, I-Shou University, Kaohsiung 84001, Taiwan, R.O.C., Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C., Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
  • Pages: 416-422
    |
    Published online on: September 11, 2017
       https://doi.org/10.3892/br.2017.979
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Abstract

Pluchea indica (L.) Less. is a perennial plant known for its versatile uses in traditional medicine. Previous findings have shown that the extracts of Pluchea indica possess significant anti-inflammatory, anti-ulcer and anti-tuberculosis activity. The aim of this study was to demonstrate the anticancer activity of the hexane fraction of P. indica root extract (H-PIRE) in human glioblastoma cells using flow cytometric and western blot analysis. The results shoewd that, H-PIRE suppressed the growth of glioblastoma cells in a dose-dependent manner. H-PIRE treatment markedly decreased the population of cells in S and G2/M phases. The significant upregulation of acidic vesicular organelle (AVO) was detected during H-PIRE treatment. The expression levels of microtubule-associated light chain 3-II (LC3-II) protein, phosphorylated JNK and phosphorylated p38 were significantly increased, confirming the occurrence of autophagy during the process. Finally, the combination treatment of H-PIRE and LY294002, a pan PI3K inhibitor, further decreased cell viability, suggesting an additive anticancer effect. Taken together, our results suggest that H-PIRE suppresses the proliferation of glioblastoma cells by inducing cell cycle arrest and autophagy.
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Copy and paste a formatted citation
Spandidos Publications style
Cho C, Lee Y, Tseng C, Cho J, Cheng Y, Wang KW, Chen H, Chiou S, Chou C, Hong Y, Hong Y, et al: Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomed Rep 7: 416-422, 2017.
APA
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K. ... Hong, Y. (2017). Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomedical Reports, 7, 416-422. https://doi.org/10.3892/br.2017.979
MLA
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K., Chen, H., Chiou, S., Chou, C., Hong, Y."Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells". Biomedical Reports 7.5 (2017): 416-422.
Chicago
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K., Chen, H., Chiou, S., Chou, C., Hong, Y."Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells". Biomedical Reports 7, no. 5 (2017): 416-422. https://doi.org/10.3892/br.2017.979
Copy and paste a formatted citation
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Spandidos Publications style
Cho C, Lee Y, Tseng C, Cho J, Cheng Y, Wang KW, Chen H, Chiou S, Chou C, Hong Y, Hong Y, et al: Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomed Rep 7: 416-422, 2017.
APA
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K. ... Hong, Y. (2017). Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomedical Reports, 7, 416-422. https://doi.org/10.3892/br.2017.979
MLA
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K., Chen, H., Chiou, S., Chou, C., Hong, Y."Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells". Biomedical Reports 7.5 (2017): 416-422.
Chicago
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K., Chen, H., Chiou, S., Chou, C., Hong, Y."Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells". Biomedical Reports 7, no. 5 (2017): 416-422. https://doi.org/10.3892/br.2017.979
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