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Article

Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release

  • Authors:
    • Haruhiko Tokuda
    • Gen Kuroyanagi
    • Takashi Onuma
    • Yukiko Enomoto
    • Tomoaki Doi
    • Hiroki Iida
    • Takanobu Otsuka
    • Shinji Ogura
    • Toru Iwama
    • Kumi Kojima
    • Osamu Kozawa
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory/Biobank of Medical Genome Center, National Center for Geriatrics and Gerontology, Obu, Aichi 474‑8511, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Neurosurgery, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467‑8601, Japan
  • Pages: 365-372
    |
    Published online on: February 6, 2018
       https://doi.org/10.3892/br.2018.1058
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Abstract

It has been previously reported that HSP27 is released from platelets in type 2 diabetes mellitus (DM) patients according to phosphorylation. In the present study, we investigated the effect of ristocetin, a glycoprotein (GP)Ib/IX/V activator, on the release of HSP27 and the effect of anti‑platelet agents, such as acetylsalicylic acid (ASA), on this release. Forty-six patients with type 2 DM were recruited, and classified into two groups depending on administration of anti‑platelet agents, resulting in 31 patients without these agents (control group) and 15 patients with them (anti‑platelet group). Ristocetin potently induced the aggregation of platelets in the two groups. Ristocetin‑induced changes of the area under the curve of light transmittance and the ratio of the size of platelet aggregates in the anti‑platelet group were slightly different from those in the control group. On the other hand, the levels of phosphorylated‑HSP27 induced by ristocetin in the platelets from a patient of the anti‑platelet group taking ASA were significantly lower than those from a patient of the control group. The levels of HSP27 release from the ristocetin‑stimulated platelets were significantly correlated with the levels of phosphorylated‑HSP27 in the platelets from patients in the two groups. The levels of HSP27 release and those of platelet‑derived growth factor‑AB (PDGF‑AB) secretion stimulated by ristocetin in the anti‑platelet group were lower than those in the control group. In addition, the levels of HSP27 release and those of PDGF‑AB secretion stimulated by ADP in the anti‑platelet group were lower than those in the control group. These results strongly suggest that anti‑platelet agents inhibit the HSP27 release from platelets stimulated by ristocetin but not the aggregation in type 2 DM patients.
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Copy and paste a formatted citation
Spandidos Publications style
Tokuda H, Kuroyanagi G, Onuma T, Enomoto Y, Doi T, Iida H, Otsuka T, Ogura S, Iwama T, Kojima K, Kojima K, et al: Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release. Biomed Rep 8: 365-372, 2018.
APA
Tokuda, H., Kuroyanagi, G., Onuma, T., Enomoto, Y., Doi, T., Iida, H. ... Kozawa, O. (2018). Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release. Biomedical Reports, 8, 365-372. https://doi.org/10.3892/br.2018.1058
MLA
Tokuda, H., Kuroyanagi, G., Onuma, T., Enomoto, Y., Doi, T., Iida, H., Otsuka, T., Ogura, S., Iwama, T., Kojima, K., Kozawa, O."Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release". Biomedical Reports 8.4 (2018): 365-372.
Chicago
Tokuda, H., Kuroyanagi, G., Onuma, T., Enomoto, Y., Doi, T., Iida, H., Otsuka, T., Ogura, S., Iwama, T., Kojima, K., Kozawa, O."Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release". Biomedical Reports 8, no. 4 (2018): 365-372. https://doi.org/10.3892/br.2018.1058
Copy and paste a formatted citation
x
Spandidos Publications style
Tokuda H, Kuroyanagi G, Onuma T, Enomoto Y, Doi T, Iida H, Otsuka T, Ogura S, Iwama T, Kojima K, Kojima K, et al: Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release. Biomed Rep 8: 365-372, 2018.
APA
Tokuda, H., Kuroyanagi, G., Onuma, T., Enomoto, Y., Doi, T., Iida, H. ... Kozawa, O. (2018). Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release. Biomedical Reports, 8, 365-372. https://doi.org/10.3892/br.2018.1058
MLA
Tokuda, H., Kuroyanagi, G., Onuma, T., Enomoto, Y., Doi, T., Iida, H., Otsuka, T., Ogura, S., Iwama, T., Kojima, K., Kozawa, O."Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release". Biomedical Reports 8.4 (2018): 365-372.
Chicago
Tokuda, H., Kuroyanagi, G., Onuma, T., Enomoto, Y., Doi, T., Iida, H., Otsuka, T., Ogura, S., Iwama, T., Kojima, K., Kozawa, O."Ristocetin induces phosphorylated‑HSP27 (HSPB1) release from the platelets of type 2 DM patients: Anti‑platelet agent‑effect on the release". Biomedical Reports 8, no. 4 (2018): 365-372. https://doi.org/10.3892/br.2018.1058
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