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Article

Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia

  • Authors:
    • Nootchanat Mairuae
    • Poonlarp Cheepsunthorn
  • View Affiliations / Copyright

    Affiliations: Biomedical Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
  • Pages: 359-364
    |
    Published online on: February 9, 2018
       https://doi.org/10.3892/br.2018.1062
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Abstract

Iron accumulation in activated microglia has been consistently reported in neurodegenerative diseases. Previous results suggest that these cells facilitate neuroinflammation leading to neuronal cell death. Therefore, chemical compounds that alleviate the activation of iron‑rich microglia may result in neuroprotection. In the present study, the effect of valproic acid (VPA) on microglial activation under iron‑rich conditions was investigated. BV‑2 microglial cells were exposed to lipopolysaccharide (LPS; 1 µg/ml) and iron (300 µg/ml) with or without VPA (1.6 mM). The results demonstrated that VPA attenuated the activation of iron‑rich BV2 cells induced by LPS by down‑regulating the mRNA expression of inducible nitric oxide (NO) synthase and interleukin 1β (IL‑1β; P<0.01), to ultimately reduce the production of NO and IL‑1β (P<0.01). These events were accompanied by an attenuation in the nuclear translocation of nuclear factor‑κB p65 subunit (P<0.01). These findings suggest that VPA may be therapeutically useful for attenuating the activation of iron‑rich microglia.
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Copy and paste a formatted citation
Spandidos Publications style
Mairuae N and Cheepsunthorn P: Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia. Biomed Rep 8: 359-364, 2018.
APA
Mairuae, N., & Cheepsunthorn, P. (2018). Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia. Biomedical Reports, 8, 359-364. https://doi.org/10.3892/br.2018.1062
MLA
Mairuae, N., Cheepsunthorn, P."Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia". Biomedical Reports 8.4 (2018): 359-364.
Chicago
Mairuae, N., Cheepsunthorn, P."Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia". Biomedical Reports 8, no. 4 (2018): 359-364. https://doi.org/10.3892/br.2018.1062
Copy and paste a formatted citation
x
Spandidos Publications style
Mairuae N and Cheepsunthorn P: Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia. Biomed Rep 8: 359-364, 2018.
APA
Mairuae, N., & Cheepsunthorn, P. (2018). Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia. Biomedical Reports, 8, 359-364. https://doi.org/10.3892/br.2018.1062
MLA
Mairuae, N., Cheepsunthorn, P."Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia". Biomedical Reports 8.4 (2018): 359-364.
Chicago
Mairuae, N., Cheepsunthorn, P."Valproic acid attenuates nitric oxide and interleukin‑1β production in lipopolysaccharide‑stimulated iron‑rich microglia". Biomedical Reports 8, no. 4 (2018): 359-364. https://doi.org/10.3892/br.2018.1062
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