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Characterization of human dental pulp cells grown in chemically defined serum‑free medium

  • Authors:
    • Sakiko Fujii
    • Katsumi Fujimoto
    • Noriko Goto
    • Yoshimitsu Abiko
    • Asayo Imaoka
    • Jinchang Shao
    • Kazuko Kitayama
    • Masami Kanawa
    • Agung Sosiawan
    • Ketut Suardita
    • Fusanori Nishimura
    • Yukio Kato
  • View Affiliations / Copyright

    Affiliations: Department of Dental Science for Health Promotion, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734‑8553, Japan, Department of Dental and Medical Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734‑8553, Japan, Department of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734‑8553, Japan, Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271‑8587, Japan, Two Cells Co., Ltd., Hiroshima 734‑0816, Japan, Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima 734‑8553, Japan, Department of Dental Public Health, Faculty of Dental Medicine, Airlangga University, Surabaya, East Java 60132, Indonesia, Department of Conservative Dentistry, Faculty of Dental Medicine, Airlangga University, Surabaya, East Java 60132, Indonesia
    Copyright: © Fujii et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 350-358
    |
    Published online on: February 16, 2018
       https://doi.org/10.3892/br.2018.1066
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Abstract

Dental pulp cells (DPCs) are promising candidates for use as transplantable cells in regenerative medicine. However, ex vivo expansion of these cells typically requires culture media containing fetal bovine serum, which may cause infection and immunological reaction following transplantation. In addition, the proliferation and differentiation of DPCs markedly depend upon serum batches. Therefore, the present study examined whether DPCs could be expanded under serum‑free conditions. DPCs obtained from four donors were identified to proliferate actively in the serum‑free medium, STK2, when compared with those cells in control medium (Dulbecco's modified Eagle's medium containing 10% serum). The high proliferative potential with STK2 was maintained through multiple successive culture passages. DNA microarray analyses demonstrated that the gene expression profile of DPCs grown in STK2 was similar to that of cells grown in the control medium; however, a number of genes related to cell proliferation, including placental growth factor and inhibin‑βE, were upregulated in the STK2 cultures. Following induction of osteogenesis, DPCs grown in STK2 induced alkaline phosphatase activity and calcification at higher levels compared with the control medium cultures, indicating maintenance of differentiation potential in STK2. This serum‑free culture system with DPCs may have applications in further experimental studies and as a clinical strategy in regenerative medicine.
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1 

Gronthos S, Mankani M, Brahim J, Robey PG and Shi S: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 97:pp. 13625–13630. 2000; View Article : Google Scholar : PubMed/NCBI

2 

Stanislawski L, Carreau JP, Pouchelet M, Chen ZH and Goldberg M: In vitro culture of human dental pulp cells: Some aspects of cells emerging early from the explant. Clin Oral Investig. 1:131–140. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Couble ML, Farges JC, Bleicher F, Perrat-Mabillon B, Boudeulle M and Magloire H: Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int. 66:129–138. 2000. View Article : Google Scholar : PubMed/NCBI

4 

He H, Yu J, Liu Y, Lu S, Liu H, Shi J and Jin Y: Effects of FGF2 and TGFbeta1 on the differentiation of human dental pulp stem cells in vitro. Cell Biol Int. 32:827–834. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Iohara K, Zheng L, Ito M, Tomokiyo A, Matsushita K and Nakashima M: Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells. 24:2493–2503. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Iohara K, Zheng L, Wake H, Ito M, Nabekura J, Wakita H, Nakamura H, Into T, Matsushita K and Nakashima M: A novel stem cell source for vasculogenesis in ischemia: Subfraction of side population cells from dental pulp. Stem Cells. 26:2408–2418. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Iohara K, Imabayashi K, Ishizaka R, Watanabe A, Nabekura J, Ito M, Matsushita K, Nakamura H and Nakashima M: Complete pulp regeneration after pulpectomy by transplantation of CD105+ stem cells with stromal cell-derived factor-1. Tissue Eng Part A. 17:1911–1920. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Min JH, Ko SY, Cho YB, Ryu CJ and Jang YJ: Dentinogenic potential of human adult dental pulp cells during the extended primary culture. Hum Cell. 24:43–50. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Shi S, Bartold PM, Miura M, Seo BM, Robey PG and Gronthos S: The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res. 8:191–199. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Sloan AJ and Waddington RJ: Dental pulp stem cells: What, where, how? Int J Paediatr Dent. 19:61–70. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Spath L, Rotilio V, Alessandrini M, Gambara G, De Angelis L, Mancini M, Mitsiadis TA, Vivarelli E, Naro F, Filippini A and Papaccio G: Explant-derived human dental pulp stem cells enhance differentiation and proliferation potentials. J Cell Mol Med. 14(6b): 1–1644. 2010.

12 

Tran-Hung L, Mathieu S and About I: Role of human pulp fibroblasts in angiogenesis. J Dent Res. 85:819–823. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Yamada Y, Fujimoto A, Ito A, Yoshimi R and Ueda M: Cluster analysis and gene expression profiles: A cDNA microarray system-based comparison between human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy. Biomaterials. 27:3766–3781. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Liu H, Gronthos S and Shi S: Dental pulp stem cells. Methods Enzymol. 419:99–113. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Sakai K, Yamamoto A, Matsubara K, Nakamura S, Naruse M, Yamagata M, Sakamoto K, Tauchi R, Wakao N, Imagama S, et al: Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms. J Clin Invest. 122:80–90. 2012.PubMed/NCBI

16 

Nakamura S, Yamada Y, Katagiri W, Sugito T, Ito K and Ueda M: Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp. J Endod. 35:1536–1542. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Gottipamula S, Muttigi MS, Kolkundkar U and Seetharam RN: Serum-free media for the production of human mesenchymal stromal cells: A review. Cell Prolif. 46:608–627. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Ishikawa I, Sawada R, Kato Y, Tsuji K, Shao J, Yamada T, Kato R and Tsuchiya T: Effectivity of the novel serum-free medium STK2 for proliferating human mesenchymal stem cells. Yakugaku Zasshi. 129:381–384. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Tsugeno Y, Sato F, Muragaki Y and Kato Y: Cell culture of human gingival fibroblasts, oral cancer cells and mesothelioma cells with serum-free media, STK1 and STK2. Biomed Rep. 2:644–648. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Fukuta M, Nakai Y, Kirino K, Nakagawa M, Sekiguchi K, Nagata S, Matsumoto Y, Yamamoto T, Umeda K, Heike T, et al: Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media. PLoS One. 9:e1122912014. View Article : Google Scholar : PubMed/NCBI

21 

Inoue H, Takahashi H, Hashimura M, Eshima K, Akiya M, Matsumoto T and Saegusa M: Cooperation of Sox4 with β-catenin/p300 complex in transcriptional regulation of the Slug gene during divergent sarcomatous differentiation in uterine carcinosarcoma. BMC Cancer. 16:532016. View Article : Google Scholar : PubMed/NCBI

22 

Fujii S, Fujimoto K, Goto N, Kanawa M, Kawamoto T, Pan H, Srivatanakul P, Rakdang W, Pornprasitwech J, Saskianti T, et al: Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomed Rep. 3:566–572. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Edgar R, Domrachev M and Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 30:207–210. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Sasamoto T, Fujimoto K, Kanawa M, Kimura J, Takeuchi J, Harada N, Goto N, Kawamoto T, Noshiro M, Suardita K, et al: DEC2 is a negative regulator for the proliferation and differentiation of chondrocyte lineage-committed mesenchymal stem cells. Int J Mol Med. 38:876–884. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Nakashima M, Iohara K and Sugiyama M: Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration. Cytokine Growth Factor Rev. 20:435–440. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Nakashima M, Iohara K, Murakami M, Nakamura H, Sato Y, Ariji Y and Matsushita K: Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: A pilot clinical study. Stem Cell Res Ther. 8:612017. View Article : Google Scholar : PubMed/NCBI

28 

Song M, Lee JH, Bae J, Bu Y and Kim EC: Human dental pulp stem cells are more effective than human bone marrow-derived mesenchymal stem cells in cerebral ischemic injury. Cell Transplant. 26:1001–1016. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Sawada R, Yamada T, Tsuchiya T and Matsuoka A: Microarray analysis of the effects of serum-free medium on gene expression changes in human mesenchymal stem cells during the in vitro culture. Yakugaku Zasshi. 130:1387–1393. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fujii S, Fujimoto K, Goto N, Abiko Y, Imaoka A, Shao J, Kitayama K, Kanawa M, Sosiawan A, Suardita K, Suardita K, et al: Characterization of human dental pulp cells grown in chemically defined serum‑free medium. Biomed Rep 8: 350-358, 2018.
APA
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J. ... Kato, Y. (2018). Characterization of human dental pulp cells grown in chemically defined serum‑free medium. Biomedical Reports, 8, 350-358. https://doi.org/10.3892/br.2018.1066
MLA
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J., Kitayama, K., Kanawa, M., Sosiawan, A., Suardita, K., Nishimura, F., Kato, Y."Characterization of human dental pulp cells grown in chemically defined serum‑free medium". Biomedical Reports 8.4 (2018): 350-358.
Chicago
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J., Kitayama, K., Kanawa, M., Sosiawan, A., Suardita, K., Nishimura, F., Kato, Y."Characterization of human dental pulp cells grown in chemically defined serum‑free medium". Biomedical Reports 8, no. 4 (2018): 350-358. https://doi.org/10.3892/br.2018.1066
Copy and paste a formatted citation
x
Spandidos Publications style
Fujii S, Fujimoto K, Goto N, Abiko Y, Imaoka A, Shao J, Kitayama K, Kanawa M, Sosiawan A, Suardita K, Suardita K, et al: Characterization of human dental pulp cells grown in chemically defined serum‑free medium. Biomed Rep 8: 350-358, 2018.
APA
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J. ... Kato, Y. (2018). Characterization of human dental pulp cells grown in chemically defined serum‑free medium. Biomedical Reports, 8, 350-358. https://doi.org/10.3892/br.2018.1066
MLA
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J., Kitayama, K., Kanawa, M., Sosiawan, A., Suardita, K., Nishimura, F., Kato, Y."Characterization of human dental pulp cells grown in chemically defined serum‑free medium". Biomedical Reports 8.4 (2018): 350-358.
Chicago
Fujii, S., Fujimoto, K., Goto, N., Abiko, Y., Imaoka, A., Shao, J., Kitayama, K., Kanawa, M., Sosiawan, A., Suardita, K., Nishimura, F., Kato, Y."Characterization of human dental pulp cells grown in chemically defined serum‑free medium". Biomedical Reports 8, no. 4 (2018): 350-358. https://doi.org/10.3892/br.2018.1066
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