A correction formula for neuron‑specific enolase measurement in hemolyzed neonatal serum samples

Wang,Qiang; Wang,Guangrong; Lu,Xiaolan; Guo,Xiaolan; Du,Qin; Lin,Fang; Fan,Quming; Zhang,Guoyuan; Wang,Dongsheng

doi:10.3892/br.2018.1081

May-2018 Volume 8 Issue 5

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May-2018 Volume 8 Issue 5

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Article

A correction formula for neuron‑specific enolase measurement in hemolyzed neonatal serum samples

Authors:
- Qiang Wang
- Guangrong Wang
- Xiaolan Lu
- Xiaolan Guo
- Qin Du
- Fang Lin
- Quming Fan
- Guoyuan Zhang
- Dongsheng Wang
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Affiliations: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China
Pages: 491-496
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Published online on: March 21, 2018

https://doi.org/10.3892/br.2018.1081
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Abstract

As a specific biomarker in neonatal hypoxic‑ischemic encephalopathy (HIE), the measurement of neuron‑specific enolase (NSE) has been advocated as a predictor of outcome in neurological injury. However, the measured levels of NSE may be influenced by hemolysis. In the current study, the change in the concentration of NSE in serum was measured by chemiluminescence prior to and following the addition of individual frozen and thawed red blood cells from 86 neonates that were collected within 24 h of birth. The changes in the concentration of NSE were compared with the changes in the concentration of hemoglobin (Hb), measured by the hemoglobin cyanide (HiCN) method, to establish a correction formula. The performance of the correction formula was evaluated by comparing the corrected concentration of NSE using the individual constants and the correction formula. The average individual constant of NSE from the 86 hemolyzed neonatal serum samples was 25.15±3.94 mg/g Hb. The concentration variation between NSE and Hb in neonatal sera could be described by the equation ΔNSEserum=1.8594+24.0670 xΔHbHiCN (r²=0.8045, P<0.001). There was no statistically significant difference in the NSE corrected results between the individual constant group and the correction formula group (Z=‑1.645, P=0.100). The linear regression formula of Hb measured with the instrumental method compared with the HiCN method was Hbinstr=0.9816xHbHiCN+0.5596 (r²=0.9924, P<0.001). Based on these regression analyses, the correction formula for NSE in hemolyzed neonatal serum was determined as NSEcorr=NSEmeas‑24.0670xHbHiCN‑1.8594 or NSEcorr=NSEmeas‑24.5181xHbinstr+11.8609. In conclusion, hemolysis has a substantial influence on the accurate measurement of NSE in neonatal serum samples. For hemolyzed neonatal serum samples, correcting the NSE results using a correction formula is essential to evaluate the severity of neonatal hypoxic ischemic encephalopathy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A correction formula for neuron‑specific enolase measurement in hemolyzed neonatal serum samples

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