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Article

Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression

  • Authors:
    • Masoumeh Gholinejad
    • Iraj Jafari Anarkooli
    • Amirhossein Taromchi
    • Alireza Abdanipour
  • View Affiliations / Copyright

    Affiliations: Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan 45139‑56184, Iran, Department of Anatomy, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan 45139‑56184, Iran
  • Pages: 439-446
    |
    Published online on: March 27, 2018
       https://doi.org/10.3892/br.2018.1083
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Abstract

Overproduction of free radicals during oxidative stress induces damage to key biomolecules and activates programed cell death pathways. Neuronal cell death in the nervous system leads to a number of neurodegenerative diseases. The aim of the present study was to evaluate the neuroprotective effect of adenosine on inhibition of apoptosis induced by hydrogen peroxide (H2O2) in bone marrow‑derived neural stem cells (B‑dNSCs), with focus on its regulatory effect on the expression of mammalian sterile 20‑like kinase 1 (Mst1), as a novel proapoptotic kinase. B‑dNSCs were exposed to adenosine at different doses (2, 4, 6, 8 and 10 µM) for 48 h followed by 125 µM H2O2 for 30 min. Using MTT, terminal deoxynucleotidyl transferase dUTP nick‑end labeling and real‑time reverse transcription polymerase chain reaction assays, the effects of adenosine on cell survival, apoptosis and Mst1, nuclear factor (erythroid‑derived 2)‑like 2 and B‑cell lymphoma 2 and adenosine A1 receptor expression were evaluated in pretreated B‑dNSCs compared with controls (cells treated with H2O2 only). Firstly, results of the MTT assay indicated 6 µM adenosine to be the most protective dose in terms of promotion of cell viability. Subsequent assays using this dosage indicated that apoptosis rate and Mst1 expression in B‑dNSCs pretreated with 6 µM adenosine were significantly decreased compared with the control group. These findings suggest that adenosine protects B‑dNSCs against oxidative stress‑induced cell death, and therefore, that it may be used to promote the survival rate of B‑dNSCs and as a candidate for the treatment of oxidative stress‑mediated neurological diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Gholinejad M, Jafari Anarkooli I, Taromchi A and Abdanipour A: Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8: 439-446, 2018.
APA
Gholinejad, M., Jafari Anarkooli, I., Taromchi, A., & Abdanipour, A. (2018). Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomedical Reports, 8, 439-446. https://doi.org/10.3892/br.2018.1083
MLA
Gholinejad, M., Jafari Anarkooli, I., Taromchi, A., Abdanipour, A."Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression". Biomedical Reports 8.5 (2018): 439-446.
Chicago
Gholinejad, M., Jafari Anarkooli, I., Taromchi, A., Abdanipour, A."Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression". Biomedical Reports 8, no. 5 (2018): 439-446. https://doi.org/10.3892/br.2018.1083
Copy and paste a formatted citation
x
Spandidos Publications style
Gholinejad M, Jafari Anarkooli I, Taromchi A and Abdanipour A: Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8: 439-446, 2018.
APA
Gholinejad, M., Jafari Anarkooli, I., Taromchi, A., & Abdanipour, A. (2018). Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomedical Reports, 8, 439-446. https://doi.org/10.3892/br.2018.1083
MLA
Gholinejad, M., Jafari Anarkooli, I., Taromchi, A., Abdanipour, A."Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression". Biomedical Reports 8.5 (2018): 439-446.
Chicago
Gholinejad, M., Jafari Anarkooli, I., Taromchi, A., Abdanipour, A."Adenosine decreases oxidative stress and protects H2O2‑treated neural stem cells against apoptosis through decreasing Mst1 expression". Biomedical Reports 8, no. 5 (2018): 439-446. https://doi.org/10.3892/br.2018.1083
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