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Article

Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia

  • Authors:
    • Guangbin Zhu
    • Changying Xie
    • Zhonghua Yang
    • Yongzhi Wang
    • Dong Chen
    • Xinghuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Daqing Oilfield General Hospital, Daqing, Heilongjiang 163000, P.R. China, Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 529-534
    |
    Published online on: April 23, 2018
       https://doi.org/10.3892/br.2018.1089
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Abstract

The present study aimed to determine whether the expression of transient receptor potential channel 5 (TRPC5) protein is altered in spermatozoa of patients with varicocele‑associated asthenozoospermia. TRPC5 expression in spermatozoa was determined by polymerase chain reaction and western blotting analyses, and indirect immunofluorescence was used for identification and immunolocalization of the TRPC5 channel in human sperm. Sperm motility and superoxide dismutase (SOD) activity were also determined with a computer‑assisted semen analysis system and assay kit, respectively. Compared with levels in control subjects, it was identified that TRPC5 protein expression, SOD activity and cellular motility in the sperm of patients with varicocele‑associated asthenozoospermia were reduced (P<0.001). Furthermore, the expression of TRPC5 was positively correlated with sperm motility (r=0.781, P<0.001) and SOD activity (r=0.933, P<0.001), indicated by partial correlation analysis. The present study may provide a novel target for the study and treatment of varicocele‑associated asthenozoospermia.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu G, Xie C, Yang Z, Wang Y, Chen D and Wang X: Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia. Biomed Rep 8: 529-534, 2018.
APA
Zhu, G., Xie, C., Yang, Z., Wang, Y., Chen, D., & Wang, X. (2018). Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia. Biomedical Reports, 8, 529-534. https://doi.org/10.3892/br.2018.1089
MLA
Zhu, G., Xie, C., Yang, Z., Wang, Y., Chen, D., Wang, X."Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia". Biomedical Reports 8.6 (2018): 529-534.
Chicago
Zhu, G., Xie, C., Yang, Z., Wang, Y., Chen, D., Wang, X."Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia". Biomedical Reports 8, no. 6 (2018): 529-534. https://doi.org/10.3892/br.2018.1089
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu G, Xie C, Yang Z, Wang Y, Chen D and Wang X: Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia. Biomed Rep 8: 529-534, 2018.
APA
Zhu, G., Xie, C., Yang, Z., Wang, Y., Chen, D., & Wang, X. (2018). Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia. Biomedical Reports, 8, 529-534. https://doi.org/10.3892/br.2018.1089
MLA
Zhu, G., Xie, C., Yang, Z., Wang, Y., Chen, D., Wang, X."Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia". Biomedical Reports 8.6 (2018): 529-534.
Chicago
Zhu, G., Xie, C., Yang, Z., Wang, Y., Chen, D., Wang, X."Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia". Biomedical Reports 8, no. 6 (2018): 529-534. https://doi.org/10.3892/br.2018.1089
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