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Article

Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats

  • Authors:
    • Saman Sargazi
    • Hamidreza Galavi
    • Sadegh Zarei
  • View Affiliations / Copyright

    Affiliations: Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Razavi Khorasan 9177948974, Iran, Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743181, Iran, Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd 8916978477, Iran
  • Pages: 565-570
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    Published online on: April 23, 2018
       https://doi.org/10.3892/br.2018.1090
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Abstract

Diazinon is an organophosphate toxicant that has been identified to induce oxidative stress within biological systems through altering biochemical and hematological indices, by generating free radicals while deteriorating the cellular antioxidant defense system. The present study was designed to evaluate the effectiveness of tert‑butylhydroquinone (TBHQ), a synthetic food additive, in serving as an antioxidant against sub‑lethal diazinon‑induced oxidative stress in male Wistar rats. Animals were randomly divided into 4 groups: Control (treated with corn oil as a vehicle), diazinon (10 mg/kg/day), TBHQ (0.028 g/kg of diet) and a combination group of TBHQ (0.028 g/kg of diet) plus diazinon (10 mg/kg/day). All animals were treated orally once a day by gastric tube and treatments were continued for 7 weeks. Hematological indices, including red blood cell (RBC) indices and white blood cell (WBC) and platelet counts, were measured at the end of the treatment schedule. On comparison of the treatment groups with the untreated control group, RBC count, hemoglobin (Hb), hematocrit (Hct), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were significantly decreased in the diazinon and TBHQ+diazinon groups, while MCHC in the TBHQ group and MCV in the diazinon and TBHQ+diazinon groups were significantly increased. When the diazinon and TBHQ+diazinon groups were compared with the TBHQ group, RBC, Hb, Hct, and MCHC were significantly decreased whereas MCV was increased in both groups. Additionally, when comparing the TBHQ+diazinon group with the diazinon group, MCHC was determined to be significantly decreased. The results of the current experiments suggested that TBHQ could not efficiently protect blood cells against diazinon toxicity.
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Copy and paste a formatted citation
Spandidos Publications style
Sargazi S, Galavi H and Zarei S: Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats. Biomed Rep 8: 565-570, 2018.
APA
Sargazi, S., Galavi, H., & Zarei, S. (2018). Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats. Biomedical Reports, 8, 565-570. https://doi.org/10.3892/br.2018.1090
MLA
Sargazi, S., Galavi, H., Zarei, S."Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats". Biomedical Reports 8.6 (2018): 565-570.
Chicago
Sargazi, S., Galavi, H., Zarei, S."Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats". Biomedical Reports 8, no. 6 (2018): 565-570. https://doi.org/10.3892/br.2018.1090
Copy and paste a formatted citation
x
Spandidos Publications style
Sargazi S, Galavi H and Zarei S: Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats. Biomed Rep 8: 565-570, 2018.
APA
Sargazi, S., Galavi, H., & Zarei, S. (2018). Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats. Biomedical Reports, 8, 565-570. https://doi.org/10.3892/br.2018.1090
MLA
Sargazi, S., Galavi, H., Zarei, S."Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats". Biomedical Reports 8.6 (2018): 565-570.
Chicago
Sargazi, S., Galavi, H., Zarei, S."Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats". Biomedical Reports 8, no. 6 (2018): 565-570. https://doi.org/10.3892/br.2018.1090
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