Silica nanoparticles of microrods enter lung epithelial cells

Tschernig,Thomas; Fischer,Thorben; Grissmer,Alexander; Beckmann,Anja; Meier,Carola; Lipp,Peter; Schneider,Marc

doi:10.3892/br.2018.1117

August-2018 Volume 9 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August-2018 Volume 9 Issue 2

Full Size Image

Article Open Access

Silica nanoparticles of microrods enter lung epithelial cells

Authors:
- Thomas Tschernig
- Thorben Fischer
- Alexander Grissmer
- Anja Beckmann
- Carola Meier
- Peter Lipp
- Marc Schneider
View Affiliations / Copyright

Affiliations: Institute of Anatomy and Cell Biology, Saarland University, D‑66421 Homburg/Saar, Germany, Department of Pharmacy, Biopharmaceutics and Pharmaceutical Technology, Saarland University, D‑66123 Saarbrücken, Germany

Copyright: © Tschernig et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 156-160
|
Published online on: June 26, 2018

https://doi.org/10.3892/br.2018.1117
Expand metrics +

Abstract

A novel type of microparticle has recently been engineered. It consists of amorphous silica nanoparticles and has a corncob‑like shape. It has already been demonstrated in vivo that alveolar macrophages in the lung are able to engulf this particulate carrier and that it also functions successfully as a gene delivery system. This subsequently raises the question as to whether epithelial cells may also be possible targets for these microrods. For this purpose, the alveolar epithelial cell line A549 was used presently. The epithelial character of these confluent cells was documented by the presence of tight junctions using a freeze‑fracture technique and transmission electron microscopy. A toxic effect of the particles incubated with these cells was largely excluded. The interaction of the microparticles with the epithelial cells was observed using confocal microscopy and live cell imaging. Interestingly, the particles entered the epithelial cells within hours. After 1 day, the intracellular particles began to disaggregate and release the silica nanoparticles. Thus, even epithelial cells may serve as targets for this novel carrier and gene delivery system. This is particularly important since safe and effective gene delivery remains an unsolved problem. In addition, delivery of anti‑cancer and anti‑infective drugs may be an application of this novel particulate carrier.

View Figures

View References

1	Armstrong DK, Cunningham S, Davies JC and Alton EW: Gene therapy in cystic fibrosis. Arch Dis Child. 99:465–468. 2014. View Article : Google Scholar : PubMed/NCBI
2	Möhwald M, Pinnapireddy SR, Wonnenberg B, Pourasghar M, Jurisic M, Jung A, Fink-Straube C, Tschernig T, Bakowsky U and Schneider M: Aspherical, Nanostructured Microparticles for Targeted Gene Delivery to Alveolar Macrophages. Adv Healthc Mater. (In press).
3	Kohler D, Schneider M, Krüger M, Lehr C-M, Möhwald H and Wang D: Template-assisted polyelectrolyte encapsulation of nanoparticles into dispersible, hierarchically nanostructured microfibers. Adv Mater. 23:1376–1379. 2011. View Article : Google Scholar : PubMed/NCBI
4	Lieber M, Smith B, Szakal A, Nelson-Rees W and Todaro G: A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 17:62–70. 1976. View Article : Google Scholar : PubMed/NCBI
5	Oberhofer M, Tian Q, Ruppenthal S, Wegener S, Reil JC, Körbel C, Hammer K, Menger M, Neuberger HR, Kaestner L, et al: Calcium dysregulation in ventricular myocytes from mice expressing constitutively active Rac1. Cell Calcium. 54:26–36. 2013. View Article : Google Scholar : PubMed/NCBI
6	Rash JE, Duffy HS, Dudek FE, Bilhartz BL, Whalen LR and Yasumura T: Grid-mapped freeze-fracture analysis of gap junctions in gray and white matter of adult rat central nervous system, with evidence for a “panglial syncytium” that is not coupled to neurons. J Comp Neurol. 388:265–292. 1997. View Article : Google Scholar : PubMed/NCBI
7	Diler E, Schwarz M, Nickels R, Menger MD, Beisswenger C, Meier C and Tschernig T: Influence of external calcium and thapsigargin on the uptake of polystyrene beads by the macrophage-like cell lines U937 and MH-S. BMC Pharmacol Toxicol. 15:162014. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Silica nanoparticles of microrods enter lung epithelial cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles