Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
October-2018 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2018 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Preparation of Astragalus membranaceus lectin and evaluation of its biological function

  • Authors:
    • Chong-Zhi Bai
    • Jian-Qing Hao
    • Xu-Liang Hao
    • Ma-Li Feng
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Chinese Medicine Hospital of Shanxi Province, Taiyuan, Shanxi 030012, P.R. China, Institute of Biotechnology, School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi 030012, P.R. China
  • Pages: 345-349
    |
    Published online on: July 18, 2018
       https://doi.org/10.3892/br.2018.1132
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Astragalus membranaceus lectin (AML) was abstracted as a supposedly novel agglutinin of 67 kDa from the seeds of Astragalus membranaceus. The seeds of Astragalus membranaceus were treated with acetate, ammonium sulfate precipitation, and purified by HiTrap SP XL ion column and Superdex G25 gel filtration chromatography to obtain the AML. AML contained 16.4% sugar, ~70% polar amino acids and ~30% hydrophobic amino acids. The AML exhibited agglutination activity toward human and animal erythrocytes, particularly human blood type O and rabbit erythrocytes. It also exhibited acid/alkali resistance and thermal denaturation above 64˚C. Compared with human normal liver HL-7702 cells, different concentrations of AML (6.25, 12.50, 25.00 and 50.00 µg/ml) exhibited superior inhibitory effects on the growth of SGC-7901, HepG2 and H22 carcinoma cell lines, and displayed marked antibacterial effects on bacteria; the half maximal inhibitory concentration for B. dysenteriae, S. aureus and E. coli were 85.4, 80.2 and 65.3 µg/ml, respectively.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jin M, Zhao K, Huang Q and Shang P: Structural features and biological activities of the polysaccharides from Astragalus membranaceus. Int J Biol Macromol. 64:257–266. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Shao BM, Xu W, Dai H, Tu P, Li Z and Gao XM: A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochem Biophys Res Commun. 320:1103–1111. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Cho WC and Leung KN: In vitro and in vivo Antitumor effects of Astragalus membranaceus. Cancer Lett. 252:43–54. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Vandenborre G, Smagghe G and Van Damme EJ: Plant lectins as defense proteins against phytophagous insects. Phytochemistry. 72:1538–1550. 2011. View Article : Google Scholar : PubMed/NCBI

5 

de Vasconcelos MA, Cunha CO, Arruda FV, Carneiro VA, Mercante FM, do Nascimento Neto LG, de Sousa GS, Rocha BA, Teixeira EH, Cavada BS, et al: : Lectin from Canavalia brasiliensis seeds (ConBr) is a valuable biotechnological tool to stimulate the growth of Rhizobium tropici in vitro. Molecules. 17:5244–5254. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Balzarini J, Neyts J, Schols D, Hosoya M, Van Damme E, Peumans W and De Clercq E: The mannose-specific plant lectins from Cymbidium hybrid and Epipactis helleborine and the (N-acetylglucosamine)n-specific plant lectin from Urtica dioica are potent and selective inhibitors of human immunodeficiency virus and cytomegalovirus replication in vitro. Antiviral Res. 18:191–207. 1992. View Article : Google Scholar : PubMed/NCBI

7 

Zuo Z, Fan H, Wang X, Zhou W and Li L: Purification and characterization of a novel plant lectin from Pinellia ternata with antineoplastic activity. Springerplus. 1:132012. View Article : Google Scholar : PubMed/NCBI

8 

Peumans WJ and Van Damme EJ: Lectins as plant defense proteins. Plant Physiol. 109:347–352. 1995. View Article : Google Scholar : PubMed/NCBI

9 

Kaltner H, García Caballero G, Ludwig AK, Manning JC and Gabius HJ: From glycophenotyping by (plant) lectin histochemistry to defining functionality of glycans by pairing with endogenous lectins. Histochem Cell Biol. 149:547–568. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Liu XQ, Wu H, Yu HL, Zhao TF, Pan YZ and Shi RJ: Purification of a lectin from Arisaema erubescens (Wall.) schott and its pro-inflammatory effects. Molecules. 16:9480–9494. 201111 View Article : Google Scholar : PubMed/NCBI

11 

Ye XY, Ng TB, Tsang PW and Wang J: Isolation of a homodimeric lectin with antifungal and antiviral activities from red kidney bean (Phaseolus vulgaris) seeds. J Protein Chem. 20:367–375. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Heinrich EL, Welty LA, Banner LR and Oppenheimer SB: Direct targeting of cancer cells: A multiparameter approach. Acta Histochem. 107:335–344. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Bruce A: Guide for the Care and Use of Laboratory Animals. National Academies Press; Washington: pp. 85–123. 1996

14 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

15 

Qin XZ, Yang XP and Chen J: Hemagglutinins of higher fungi in Altay. Xinjiang Nongye Kexue. 53:142–148. 2016.(In Chinese).

16 

Dubois M, Gilles KA, Hamilton JK, Rebers PA and Smith F: Colorimetric method for determination of sugars and related substances. Anal Chem. 28:350–356. 1956. View Article : Google Scholar

17 

Chou TC: The median-effect principle and the combination index for quantitation of synergism and antagonism. Synergism and Antagonism in Chemotherapy. Chou TC and Rideout DC: Academic Press; San Diego, CA: pp. 61–102. 1991

18 

Barbul A, Sisto DA, Wasserkrug HL and Efron G: Arginine stimulates lymphocyte immune response in healthy human beings. Surgery. 90:244–251. 1981.PubMed/NCBI

19 

Zhu LF, Yan QJ, Jiang ZQ and Huang LH: Isolation and purification of a lectin from roots of Astragalus membranaceus. Chin Tradit Herbal Drugs. 41:714–717. 2010.

20 

Kaur A, Singh J, Kamboj SS, Sexana AK, Pandita RM and Shamnugavel M: Isolation of an N-acetyl-D-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity. Phytochemistry. 66:1933–1940. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Vaz AF, Costa RM, Melo AM, Oliva ML, Santana LA, Silva-Lucca RA, Coelho LC and Correia MT: Biocontrol of Fusarium species by a novel lectin with low ecotoxicity isolated from Sebastiania jacobinensis. Food Chem. 119:1507–1513. 2010. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Bai C, Hao J, Hao X and Feng M: Preparation of Astragalus membranaceus lectin and evaluation of its biological function. Biomed Rep 9: 345-349, 2018.
APA
Bai, C., Hao, J., Hao, X., & Feng, M. (2018). Preparation of Astragalus membranaceus lectin and evaluation of its biological function. Biomedical Reports, 9, 345-349. https://doi.org/10.3892/br.2018.1132
MLA
Bai, C., Hao, J., Hao, X., Feng, M."Preparation of Astragalus membranaceus lectin and evaluation of its biological function". Biomedical Reports 9.4 (2018): 345-349.
Chicago
Bai, C., Hao, J., Hao, X., Feng, M."Preparation of Astragalus membranaceus lectin and evaluation of its biological function". Biomedical Reports 9, no. 4 (2018): 345-349. https://doi.org/10.3892/br.2018.1132
Copy and paste a formatted citation
x
Spandidos Publications style
Bai C, Hao J, Hao X and Feng M: Preparation of Astragalus membranaceus lectin and evaluation of its biological function. Biomed Rep 9: 345-349, 2018.
APA
Bai, C., Hao, J., Hao, X., & Feng, M. (2018). Preparation of Astragalus membranaceus lectin and evaluation of its biological function. Biomedical Reports, 9, 345-349. https://doi.org/10.3892/br.2018.1132
MLA
Bai, C., Hao, J., Hao, X., Feng, M."Preparation of Astragalus membranaceus lectin and evaluation of its biological function". Biomedical Reports 9.4 (2018): 345-349.
Chicago
Bai, C., Hao, J., Hao, X., Feng, M."Preparation of Astragalus membranaceus lectin and evaluation of its biological function". Biomedical Reports 9, no. 4 (2018): 345-349. https://doi.org/10.3892/br.2018.1132
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team