Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
October-2018 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2018 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice

  • Authors:
    • Yu Gu
    • Zhuo-Lin Qiu
    • De-Zhao Liu
    • Guo-Liang Sun
    • Ying-Chao Guan
    • Zi-Qing Hei
    • Xiang Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 291-304
    |
    Published online on: July 26, 2018
       https://doi.org/10.3892/br.2018.1135
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Diabetic peripheral neuropathy (DPN) is a common complication of diabetes mellitus (DM). The pathogenic mechanisms of DPN and the therapeutic interventions required may be distinct between type 1 (T1) and type 2 (T2) DM. However, the molecular mechanisms underlying the pathogenesis of DPN in both types of diabetes remain unclear. The aim of the current study was to identify the changes in genes and pathways associated with DPN in sciatic nerves of T1- and T2DM mice using bioinformatics analysis. The microarray profiles of sciatic nerves of T1DM (GSE11343) and T2DM (GSE27382) mouse models were downloaded from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) in each. DEGs in the two types of DM (with fold change ≥2 and P<0.05) were identified with BRB-ArrayTools. Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the Database for Annotation, Visualization and Integrated Discovery. A protein-protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins and visualized using Cytoscape. Compared with control samples, 623 and 1,890 DEGs were identified in sciatic nerves of T1- and T2DM mice, respectively. Of these, 75 genes were coordinately dysregulated in the sciatic nerves of both models. Many DEGs unique to T1DM mice were localized to the nucleoplasm and were associated with regulation of transcription processes, while many unique to T2DM mice were localized at cell junctions and were associated with ion transport. In addition, certain DEGs may be associated with the different treatment strategies used for the two types of DM. This analysis provides insight into the functional gene sets and pathways operating in sciatic nerves in T1- and T2DM. The results should improve understanding of the molecular mechanisms underlying the pathophysiology of DPN, and provide information for the development of therapeutic strategies for DPN specific to each type of DM.
View Figures

Figure 1

Figure 2

View References

1 

Vincent AM, Callaghan BC, Smith AL and Feldman EL: Diabetic neuropathy: Cellular mechanisms as therapeutic targets. Nat Rev Neurol. 7:573–583. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Tesfaye S and Selvarajah D: Advances in the epidemiology, pathogenesis and management of diabetic peripheral neuropathy. Diabetes Metab Res Rev. 28 Suppl 1:8–14. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Premkumar LS and Pabbidi RM: Diabetic peripheral neuropathy: Role of reactive oxygen and nitrogen species. Cell Biochem Biophys. 67:373–383. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Levitt NS, Stansberry KB, Wynchank S and Vinik AI: The natural progression of autonomic neuropathy and autonomic function tests in a cohort of people with IDDM. Diabetes Care. 19:751–754. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Rathmann W, Ziegler D, Jahnke M, Haastert B and Gries FA: Mortality in diabetic patients with cardiovascular autonomic neuropathy. Diabet Med. 10:820–824. 1993. View Article : Google Scholar : PubMed/NCBI

6 

OBrien PD, Sakowski SA and Feldman EL: Mouse models of diabetic neuropathy. ILAR J. 54:259–272. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Callaghan BC, Hur J and Feldman EL: Diabetic neuropathy: One disease or two? Curr Opin Neurol. 25:536–541. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Sima AA and Kamiya H: Diabetic neuropathy differs in type 1 and type 2 diabetes. Ann NY Acad Sci. 1084:235–249. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Kamiya H, Murakawa Y, Zhang W and Sima AA: Unmyelinated fiber sensory neuropathy differs in type 1 and type 2 diabetes. Diabetes Metab Res Rev. 21:448–458. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Stevens MJ, Zhang W, Li F and Sima AA: C-peptide corrects endoneurial blood flow but not oxidative stress in type 1 BB/Wor rats. Am J Physiol Endocrinol Metab. 287:E497–E505. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Qu W, Han C, Li M, Zhang J and Li L: Revealing the underlying mechanism of diabetic nephropathy viewed by microarray analysis. Exp Clin Endocrinol Diabetes. 123:353–359. 2015. View Article : Google Scholar : PubMed/NCBI

12 

He K, Lv W, Zhang Q, Wang Y, Tao L and Liu D: Gene set enrichment analysis of pathways and transcription factors associated with diabetic retinopathy using a microarray dataset. Int J Mol Med. 36:103–112. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Lacroix-Fralish ML, Tawfik VL, Tanga FY, Spratt KF and DeLeo JA: Differential spinal cord gene expression in rodent models of radicular and neuropathic pain. Anesthesiology. 104:1283–1292. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Zhang L, Qu S, Liang A, Jiang H and Wang H: Gene expression microarray analysis of the sciatic nerve of mice with diabetic neuropathy. Int J Mol Med. 35:333–339. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Wiggin TD, Kretzler M, Pennathur S, Sullivan KA, Brosius FC and Feldman EL: Rosiglitazone treatment reduces diabetic neuropathy in streptozotocin-treated DBA/2J mice. Endocrinology. 149:4928–4937. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Pande M, Hur J, Hong Y, Backus C, Hayes JM, Oh SS, Kretzler M and Feldman EL: Transcriptional profiling of diabetic neuropathy in the BKS db/db mouse: A model of type 2 diabetes. Diabetes. 60:1981–1989. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Simon R, Lam A, Li MC, Ngan M, Menenzes S and Zhao Y: Analysis of gene expression data using BRB-ArrayTools. Cancer Inform. 3:11–17. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Huang W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Huang W, Sherman BT and Lempicki RA: Bioinformatics enrichment tools: Paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res. 37:1–13. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Wang J, Ma SH, Tao R, Xia LJ, Liu L and Jiang YH: Gene expression profile changes in rat dorsal horn after sciatic nerve injury. Neurol Res. 39:176–182. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Yang YL, Xiang RL, Yang C, Liu XJ, Shen WJ, Zuo J, Chang YS and Fang FD: Gene expression profile of human skeletal muscle and adipose tissue of Chinese Han patients with type 2 diabetes mellitus. Biomed Environ Sci. 22:359–368. View Article : Google Scholar : PubMed/NCBI

22 

Sun J, Wang D and Jin T: Insulin alters the expression of components of the Wnt signaling pathway including TCF-4 in the intestinal cells. Biochim Biophys Acta. 1800:344–351. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Matsumoto K and Yokoyama S: Gene expression analysis on the liver of cholestyramine-treated type 2 diabetic model mice. Biomed Pharmacother. 64:373–378. View Article : Google Scholar : PubMed/NCBI

24 

Abdul-Rahman O, Sasvari-Szekely M, Ver A, Rosta K, Szasz BK, Kereszturi E and Keszler G: Altered gene expression profiles in the hippocampus and prefrontal cortex of type 2 diabetic rats. BMC Genomics. 13:812012. View Article : Google Scholar : PubMed/NCBI

25 

Mounier C and Posner BI: Transcriptional regulation by insulin: From the receptor to the gene. Can J Physiol Pharmacol. 84:713–724. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Schuelert N, Gorodetskaya N, Just S, Doods H and Corradini L: Electrophysiological characterization of spinal neurons in different models of diabetes type 1- and type 2-induced neuropathy in rats. Neuroscience. 291:146–154. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Echeverry S, Shi XQ and Zhang J: Characterization of cell proliferation in rat spinal cord following peripheral nerve injury and the relationship with neuropathic pain. Pain. 135:37–47. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Sun C, Zhang J, Chen L, Liu T, Xu G, Li C, Yuan W, Xu H and Su Z: IL-17 contributed to the neuropathic pain following peripheral nerve injury by promoting astrocyte proliferation and secretion of proinflammatory cytokines. Mol Med Rep. 15:89–96. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Guzen FP, de Almeida Leme RJ, de Andrade MS, de Luca BA and Chadi G: Glial cell line-derived neurotrophic factor added to a sciatic nerve fragment grafted in a spinal cord gap ameliorates motor impairments in rats and increases local axonal growth. Restor Neurol Neurosci. 27:1–16. 2009.PubMed/NCBI

30 

Chang-Chen KJ, Mullur R and Bernal-Mizrachi E: Beta-cell failure as a complication of diabetes. Rev Endocr Metab Disord. 9:329–343. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Kim B, McLean LL, Philip SS and Feldman EL: Hyperinsulinemia induces insulin resistance in dorsal root ganglion neurons. Endocrinology. 152:3638–3647. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Bian O, Zhang H, Guan Q, Sun Y and Zeng D: High-dose insulin inhibits gap junction intercellular communication in vascular smooth muscle cells. Mol Med Rep. 12:331–336. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Takenaka T, Inoue T, Okada H, Ohno Y, Miyazaki T, Chaston DJ, Hill CE and Suzuki H: Altered gap junctional communication and renal haemodynamics in Zucker fatty rat model of type 2 diabetes. Diabetologia. 54:2192–2201. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Ly LD, Xu S, Choi SK, Ha CM, Thoudam T, Cha SK, Wiederkehr A, Wollheim CB, Lee IK and Park KS: Oxidative stress and calcium dysregulation by palmitate in type 2 diabetes. Exp Mol Med. 49:e2912017. View Article : Google Scholar : PubMed/NCBI

35 

Sano T, Iwashita M, Nagayasu S, Yamashita A, Shinjo T, Hashikata A, Asano T, Kushiyama A, Ishimaru N, Takahama Y, et al: Protection from diet-induced obesity and insulin resistance in mice lacking CCL19-CCR7 signaling. Obesity (Silver Spring). 23:1460–1471. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gu Y, Qiu Z, Liu D, Sun G, Guan Y, Hei Z and Li X: Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice. Biomed Rep 9: 291-304, 2018.
APA
Gu, Y., Qiu, Z., Liu, D., Sun, G., Guan, Y., Hei, Z., & Li, X. (2018). Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice. Biomedical Reports, 9, 291-304. https://doi.org/10.3892/br.2018.1135
MLA
Gu, Y., Qiu, Z., Liu, D., Sun, G., Guan, Y., Hei, Z., Li, X."Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice". Biomedical Reports 9.4 (2018): 291-304.
Chicago
Gu, Y., Qiu, Z., Liu, D., Sun, G., Guan, Y., Hei, Z., Li, X."Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice". Biomedical Reports 9, no. 4 (2018): 291-304. https://doi.org/10.3892/br.2018.1135
Copy and paste a formatted citation
x
Spandidos Publications style
Gu Y, Qiu Z, Liu D, Sun G, Guan Y, Hei Z and Li X: Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice. Biomed Rep 9: 291-304, 2018.
APA
Gu, Y., Qiu, Z., Liu, D., Sun, G., Guan, Y., Hei, Z., & Li, X. (2018). Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice. Biomedical Reports, 9, 291-304. https://doi.org/10.3892/br.2018.1135
MLA
Gu, Y., Qiu, Z., Liu, D., Sun, G., Guan, Y., Hei, Z., Li, X."Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice". Biomedical Reports 9.4 (2018): 291-304.
Chicago
Gu, Y., Qiu, Z., Liu, D., Sun, G., Guan, Y., Hei, Z., Li, X."Differential gene expression profiling of the sciatic nerve in type 1 and type 2 diabetic mice". Biomedical Reports 9, no. 4 (2018): 291-304. https://doi.org/10.3892/br.2018.1135
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team