Evaluation of miR‑135a/b expression in endometriosis lesions

Petracco,Rafaella; Dias,Ana  Cristina de Oliveira; Taylor,Hugh  S.; Petracco,Álvaro; Badalotti,Mariângela; Michelon,João  da Rosa; Marinowic,Daniel  Rodrigo; Hentschke,Marta; de Azevedo,Pamella  Nunes; Zanirati,Gabriele; Machado,Denise  Cantarelli

doi:10.3892/br.2019.1237

Evaluation of miR‑135a/b expression in endometriosis lesions

Authors:
- Rafaella Petracco
- Ana Cristina de Oliveira Dias
- Hugh S. Taylor
- Álvaro Petracco
- Mariângela Badalotti
- João da Rosa Michelon
- Daniel Rodrigo Marinowic
- Marta Hentschke
- Pamella Nunes de Azevedo
- Gabriele Zanirati
- Denise Cantarelli Machado
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Affiliations: School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90610‑000, Brazil, Quatro G P&D Ltda., Porto Alegre, Rio Grande do Sul 90619‑900, Brazil, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University, New Haven, CT 06520‑8063, USA
Published online on: September 2, 2019 https://doi.org/10.3892/br.2019.1237
Pages: 181-187

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Abstract

The pathogenesis of endometriosis is not clear; however, microRNAs (miRNAs/miRs) are involved in the pathogenesis. miRNAs are short noncoding RNAs involved in post‑transcriptional regulation of gene expression by silencing the expression of target genes. The expression of miR‑135a/b is associated with endometrial receptivity and implantation; the expression is also associated with the expression of certain genes, including homeobox protein Hox‑A10 (HOXA‑10). The present study investigated the expression of miR‑135a/b in eutopic and ectopic endometrium tissues throughout the different phases of the menstrual cycle. Samples of ectopic endometriosis lesions and eutopic endometrium tissue from 23 patients who underwent laparoscopic surgery were obtained and analyzed. miRNA was extracted and the expression levels of miR‑135a/b were determined by reverse transcription quantitative polymerase chain reaction assays using U6 as a housekeeping control. The expression levels of miR‑135a and miR‑135b in endometriosis lesions were decreased compared with the levels in endometrium tissue. However, miR‑135a/b expression levels were increased in the secretory phase compared with the proliferative phase in endometriosis lesions. The increased expression of miR‑135a/b during the secretory phase compared with the proliferative phase suggested that these genes serve a determinant role in the homeostasis of reproductive tissue. Therefore, the expression of genes may affect endometrial functioning, impairing embryo implantation.

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