High-resolution melting for detecting KRAS mutations in colorectal cancer

  • Authors:
    • Raelson Rodrigues Miranda
    • Tiago Donizetti Silva
    • Nora Manoukian Forones
  • View Affiliations

  • Published online on: November 7, 2019     https://doi.org/10.3892/br.2019.1254
  • Pages: 269-273
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Abstract

Colorectal cancer is a leading cause of cancer that may metastasize. KRAS gene sequence of exon 2 should be examined for identification of patients that can be treated with anti-EGFR. The aim of the present study was to evaluate the efficacy of high-resolution melting (HRM) to detect KRAS mutations in colorectal cancer (CRC) tumors. The exon 2 of KRAS was amplified from 47 adenocarcinoma CRC tissues. The tumors were subjected to high-resolution melt using quantitative PCR to identify wild‑type and mutant subgroups. The results were compared to the mutations detected by next‑generation sequences (NGS). The study included 47 patients, with a mean age of 62 years, of whom 24 patients were male. Most of the patients had stage II or stage III tumors. The mean melting temperatures for the wild-type and mutated group at exon 2 were 78.13˚C and 77.28˚C, respectively (P<0.001, 95% CI = 0.11‑0.4). The sensitivity and specificity of high‑resolution melting were 83.3 and 96.6%, respectively, with a high concordance between the NGS and HRM methods for detecting KRAS mutation in exon 2 (ĸ = 0.816; P=0.625). Thus, HRM could be used as an alternative method for detecting KRAS mutations in colorectal cancer tissue.
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December-2019
Volume 11 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Miranda RR, Silva TD and Forones NM: High-resolution melting for detecting KRAS mutations in colorectal cancer. Biomed Rep 11: 269-273, 2019
APA
Miranda, R.R., Silva, T.D., & Forones, N.M. (2019). High-resolution melting for detecting KRAS mutations in colorectal cancer. Biomedical Reports, 11, 269-273. https://doi.org/10.3892/br.2019.1254
MLA
Miranda, R. R., Silva, T. D., Forones, N. M."High-resolution melting for detecting KRAS mutations in colorectal cancer". Biomedical Reports 11.6 (2019): 269-273.
Chicago
Miranda, R. R., Silva, T. D., Forones, N. M."High-resolution melting for detecting KRAS mutations in colorectal cancer". Biomedical Reports 11, no. 6 (2019): 269-273. https://doi.org/10.3892/br.2019.1254