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Article

Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer

  • Authors:
    • Shaian Tavakolian
    • Hossein Goudarzi
    • Farhad Torfi
    • Ebrahim Faghihloo
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 19839‑63113, Iran, Surgical Ward, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran 19839‑63113, Iran
  • Pages: 30-34
    |
    Published online on: November 21, 2019
       https://doi.org/10.3892/br.2019.1257
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Abstract

Given the global outbreak of breast cancer and its debilitating effect on women's health, it is not surprising that tremendous efforts have been made with an aim of shedding more light on the mechanisms involved in the pathogenesis of this type of cancer. Among the long list of risk factors associated with this malignancy, recently, the role of microRNAs (miRNAs or miRs) has turned into a hotspot for breast cancer investigations. miRNAs approximately 20 nucleotides in length and are located in either an exon or an intron, playing a role in the regulation of gene expression. In the present study, we extracted RNA from both the serum and cancerous tissue of breast cancer patients and after synthesizing the cDNA, we performed quantitative PCR to determine the expression levels of miR‑9 and miR‑192. The resulting data revealed that while the mRNA expression level of miR‑9 was significantly decreased in the breast cancer tissues, there was no noticeable change in the expression level of this miRNA in the serum samples. Likewise, we found that the marked downregulation of miR‑192 was only restricted to the cancerous tissues, but was not found in the serum of patients. Based on the meaningful downregulation of the expression of miR‑9 and miR‑192, this study provides a plausible framework for these miRNAs as effective biomarkers for breast cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Tavakolian S, Goudarzi H, Torfi F and Faghihloo E: Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer. Biomed Rep 12: 30-34, 2020.
APA
Tavakolian, S., Goudarzi, H., Torfi, F., & Faghihloo, E. (2020). Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer. Biomedical Reports, 12, 30-34. https://doi.org/10.3892/br.2019.1257
MLA
Tavakolian, S., Goudarzi, H., Torfi, F., Faghihloo, E."Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer". Biomedical Reports 12.1 (2020): 30-34.
Chicago
Tavakolian, S., Goudarzi, H., Torfi, F., Faghihloo, E."Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer". Biomedical Reports 12, no. 1 (2020): 30-34. https://doi.org/10.3892/br.2019.1257
Copy and paste a formatted citation
x
Spandidos Publications style
Tavakolian S, Goudarzi H, Torfi F and Faghihloo E: Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer. Biomed Rep 12: 30-34, 2020.
APA
Tavakolian, S., Goudarzi, H., Torfi, F., & Faghihloo, E. (2020). Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer. Biomedical Reports, 12, 30-34. https://doi.org/10.3892/br.2019.1257
MLA
Tavakolian, S., Goudarzi, H., Torfi, F., Faghihloo, E."Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer". Biomedical Reports 12.1 (2020): 30-34.
Chicago
Tavakolian, S., Goudarzi, H., Torfi, F., Faghihloo, E."Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer". Biomedical Reports 12, no. 1 (2020): 30-34. https://doi.org/10.3892/br.2019.1257
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