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Article

Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts

  • Authors:
    • Young Il Kim
    • Chan‑Yang Lee
    • Min Kyung Shin
  • View Affiliations / Copyright

    Affiliations: Medical Science Research Institute, Kyung Hee University Medical Center, Seoul 02447, Republic of Korea, Department of Dermatology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
  • Pages: 17-22
    |
    Published online on: November 21, 2019
       https://doi.org/10.3892/br.2019.1258
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Abstract

Activins are members of the transforming growth factor‑β (TGF‑β) superfamily and play important roles in proliferation, differentiation, and apoptosis of various target cells. We investigated changes of activin, activin receptor (ActR), and Smad‑signaling gene expression with increasing passage number in normal human dermal fibroblasts. The expression of mRNA and protein was measured by reverse transcription‑quantitative polymerase chain reaction and immunoblot analysis from passage numbers 5 to 15. Activin A and follistatin transcript levels increased with increasing passage number. ActR types IA, IB, IIA and IIB mRNA levels decreased at high passage number. The levels of Smad2, 3 and 4 protein decreased with increasing passage number, which also attenuated phosphorylation of Smad2 and 3 protein expression. Smad7 was enhanced with increasing passage number. These results suggest that expression of activin‑signaling in aging normal human dermal fibroblasts increases activin A and follistatin, whereas ActR‑Smad signaling is decreased.
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Copy and paste a formatted citation
Spandidos Publications style
Kim YI, Lee CY and Shin MK: Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts. Biomed Rep 12: 17-22, 2020.
APA
Kim, Y.I., Lee, C., & Shin, M.K. (2020). Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts. Biomedical Reports, 12, 17-22. https://doi.org/10.3892/br.2019.1258
MLA
Kim, Y. I., Lee, C., Shin, M. K."Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts". Biomedical Reports 12.1 (2020): 17-22.
Chicago
Kim, Y. I., Lee, C., Shin, M. K."Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts". Biomedical Reports 12, no. 1 (2020): 17-22. https://doi.org/10.3892/br.2019.1258
Copy and paste a formatted citation
x
Spandidos Publications style
Kim YI, Lee CY and Shin MK: Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts. Biomed Rep 12: 17-22, 2020.
APA
Kim, Y.I., Lee, C., & Shin, M.K. (2020). Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts. Biomedical Reports, 12, 17-22. https://doi.org/10.3892/br.2019.1258
MLA
Kim, Y. I., Lee, C., Shin, M. K."Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts". Biomedical Reports 12.1 (2020): 17-22.
Chicago
Kim, Y. I., Lee, C., Shin, M. K."Downregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts". Biomedical Reports 12, no. 1 (2020): 17-22. https://doi.org/10.3892/br.2019.1258
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