Comparison of protease and aminopeptidase activities in meconium: A pilot study

  • Authors:
    • Ewa Skarżyńska
    • Paulina Wilczyńska
    • Bartosz Kiersztyn
    • Joanna Żytyńska-Daniluk
    • Artur Jakimiuk
    • Barbara Lisowska-Myjak
  • View Affiliations

  • Published online on: June 9, 2020     https://doi.org/10.3892/br.2020.1314
  • ArticleNumber: 7
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The successive accumulation of proteases and aminopeptidases in meconium are important physiological components of the intrauterine environment in which a fetus develops. The aim of the present study was to assess the changes in the activities of these enzymes in meconium of healthy infants, and to investigate whether there were any statistically significant associations between activity of the enzymes of interest and the mode of delivery. The activities of proteases and aminopeptidases were determined in meconium portions (n=110) using the substrates BODIPY FL casein and L-leucine-7-amido-4-methylcoumarin hydrochloride, respectively. Serial meconium samples (2-5 per neonate) were collected from healthy infants born vaginally (n=14), and by a cesarean section (n=16). Protease activity (104 RFU/h) was lower in the first meconium sample compared with the final sample from the same infant (3.99±2.03 vs. 5.76±2.24, respectively, mean ± standard deviation; P=0.004). Conversely, there was no significant difference in aminopeptidase activity (103 nM/l/h) between consecutive meconium samples (P=0.702). The ratios of the first-meconium sample enzyme activity to the last-meconium sample enzyme activity were lower for proteases compared with aminopeptidases (0.76±0.48 vs. 1.35±1.04, respectively mean ± standard deviation; P=0.014), and sustained in the infants born by a cesarean section (P=0.008). Spearman's correlation coefficient analysis between the first and last meconium samples showed the correlation increased in the infants born vaginally compared with the rest of the infants (proteases, R=0.618 vs. R=0.314; aminopeptidases, R=0.688 vs. R=0.566). Aminopeptidase activity did not exhibit any notable dynamic changes during meconium accumulation in the fetal intestine. In infants born vaginally compared with those born by a cesarean section, the activity of both proteases and aminopeptidases in the first meconium sample showed an improved correlation with the activity of the final meconium sample. This may suggest that in the intrauterine environment, during accumulation of meconium in the digestive tract of the fetus, the activity and/or levels of these enzymes and the substrates they catalyze were more stable in newborns born vaginally compared with infants born by caesarean section.
View Figures
View References

Related Articles

Journal Cover

August-2020
Volume 13 Issue 2

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Skarżyńska E, Wilczyńska P, Kiersztyn B, Żytyńska-Daniluk J, Jakimiuk A and Lisowska-Myjak B: Comparison of protease and aminopeptidase activities in meconium: A pilot study. Biomed Rep 13: 7, 2020
APA
Skarżyńska, E., Wilczyńska, P., Kiersztyn, B., Żytyńska-Daniluk, J., Jakimiuk, A., & Lisowska-Myjak, B. (2020). Comparison of protease and aminopeptidase activities in meconium: A pilot study. Biomedical Reports, 13, 7. https://doi.org/10.3892/br.2020.1314
MLA
Skarżyńska, E., Wilczyńska, P., Kiersztyn, B., Żytyńska-Daniluk, J., Jakimiuk, A., Lisowska-Myjak, B."Comparison of protease and aminopeptidase activities in meconium: A pilot study". Biomedical Reports 13.2 (2020): 7.
Chicago
Skarżyńska, E., Wilczyńska, P., Kiersztyn, B., Żytyńska-Daniluk, J., Jakimiuk, A., Lisowska-Myjak, B."Comparison of protease and aminopeptidase activities in meconium: A pilot study". Biomedical Reports 13, no. 2 (2020): 7. https://doi.org/10.3892/br.2020.1314