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Article

Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats

  • Authors:
    • Nan Zhao
    • Le Mu
    • Xiaoyu Chang
    • Lingqing Zhu
    • Yao Geng
    • Guanghua Li
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, School of Public Health and Management, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, School of Nursing, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
  • Article Number: 39
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    Published online on: August 24, 2020
       https://doi.org/10.3892/br.2020.1346
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Abstract

The aim of the present study was to investigate the effects of varying intensities of heat stress on the mRNA expression levels of neuropeptide Y (NPY), proopiomelanocortin (POMC) and stress hormones in rats. To establish a rat model of heat stress, the temperature and time were adjusted in a specialized heating chamber. Sprague‑Dawley (SD) rats were randomly divided into four groups; control (CN; temperature, 24±1˚C); moderate strength 6 h (MS6; temperature, 32±1˚C time, 6 h), moderate strength 24 h (MS24; temperature, 32±1˚C; time, 24 h) and high strength 6 h (HS6; temperature, 38±1˚C; time, 6 h) groups. SD rats were exposed to heat for 14 consecutive days. The levels of heat stress‑related factors, including corticotropin‑releasing hormone (CRH), cortisol (COR), epinephrine (EPI) and heat shock protein 70 (HSP70), were measured in the rat blood using ELISA. In addition, the weight of the spleen, thymus, hypophysis and hypothalamus were determined. The mRNA expressions levels of NPY and POMC were detected using quantitative PCR. The results showed that the CRH, COR and HSP70 levels were increased in the three heat stress groups compared with the CN group. Notably, the levels of CRH, EPI and HSP70 were increased in the HS6 group compared with the CN and MS6 groups (P<0.05). Furthermore, the weights of the hypophysis and hypothalamus in the HS6 group were significantly lower compared with the CN group (P<0.05). In addition, NPY and POMC expression levels were downregulated in the MS24 group compared with the CN group. The mRNA expression levels of NPY and POMC were altered in response to different intensities of heat stress. Therefore, their levels were downregulated and upregulated following long‑time and moderate‑time heat exposure, respectively. The results of the present study suggested that the reduced mRNA expression levels of NPY may be partially responsible for the heat‑induced injuries in rats following long‑time heat exposure.
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1 

Bagath M, Krishnan G, Devaraj C, Rashamol VP, Pragna P, Lees AM and Sejian V: The impact of heat stress on the immune system in dairy cattle: A review. Res Vet Sci. 126:94–102. 2019.PubMed/NCBI View Article : Google Scholar

2 

He X, Lu Z, Ma B, Zhang L, Li J, Jiang Y, Zhou G and Gao F: Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. J Therm Biol. 81:110–117. 2019.PubMed/NCBI View Article : Google Scholar

3 

Jay O and Brotherhood JR: Occupational heat stress in Australian workplaces. Temperature (Austin). 3:394–411. 2016.PubMed/NCBI View Article : Google Scholar

4 

Baker JD, Ozsan I, Rodriguez Ospina S, Gulick D and Blair LJ: Hsp90 Heterocomplexes regulate steroid hormone receptors: From stress response to psychiatric disease. Int J Mol Sci. 20(79)2018.PubMed/NCBI View Article : Google Scholar

5 

Rimoldi S, Lasagna E, Sarti FM, Marelli SP, Cozzi MC, Bernardini G and Terova G: Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions. Meta Gene. 6:17–25. 2015.PubMed/NCBI View Article : Google Scholar

6 

Spencer RL and Deak T: A users guide to HPA axis research. Physiol Behav. 178:43–65. 2019.PubMed/NCBI View Article : Google Scholar

7 

Morales-Medina JC, Dumont Y and Quirion R: A possible role of neuropeptide Y in depression and stress. Brain Res. 1314:194–205. 2010.PubMed/NCBI View Article : Google Scholar

8 

Li Q, Bartley AF and Dobrunz LE: Endogenously released neuropeptide Y suppresses hippocampal short-term facilitation and is impaired by stress-induced anxiety. J Neurosci. 37:23–37. 2017.PubMed/NCBI View Article : Google Scholar

9 

Reichmann F and Holzer P: Neuropeptide Y: A stressful review. Neuropeptides. 55:99–109. 2016.PubMed/NCBI View Article : Google Scholar

10 

Sayed S, Van Dam NT, Horn SR, Kautz MM, Parides M, Costi S, Collins KA, Iacoviello B, Iosifescu DV, Mathé AA, et al: A randomized dose-ranging study of neuropeptide Y in patients with posttraumatic stress disorder. Int J Neuropsychopharmacol. 21:3–11. 2018.PubMed/NCBI View Article : Google Scholar

11 

Sah R and Geracioti TD: Neuropeptide Y and posttraumatic stress disorder. Mol Psychiatry. 18:646–655. 2013.PubMed/NCBI View Article : Google Scholar

12 

Backstrom T and Winberg S: Central corticotropin releasing factor and social stress. Front Neurosci. 7(117)2013.PubMed/NCBI View Article : Google Scholar

13 

Zhou JJ, Gao Y, Zhang X, Kosten TA and Li DP: Enhanced hypothalamic NMDA receptor activity contributes to hyperactivity of HPA axis in chronic stress in male rats. Endocrinology. 159:1537–1546. 2018.PubMed/NCBI View Article : Google Scholar

14 

Zhou JN and Fang H: Transcriptional regulation of corticotropin-releasing hormone gene in stress response. IBRO Rep. 5:137–146. 2018.PubMed/NCBI View Article : Google Scholar

15 

Zhang S, Lv F, Yuan Y, Fan C, Li J, Sun W and Hu J: Whole-brain mapping of monosynaptic afferent inputs to cortical CRH neurons. Front Neurosci. 13(565)2019.PubMed/NCBI View Article : Google Scholar

16 

Carney BC, Dougherty RD, Moffatt LT, Simbulan-Rosenthal CM, Shupp JW and Rosenthal DS: Promoter methylation status in pro-opiomelanocortin (POMC) does not contribute to dyspigmentation in hypertrophic scar. J Burn Care Res. 41:339–346. 2020.PubMed/NCBI View Article : Google Scholar

17 

Duque-Díaz E, Alvarez-Ojeda O and Coveñas R: Enkephalins and ACTH in the mammalian nervous system. Vitam Horm. 111:147–193. 2019.PubMed/NCBI View Article : Google Scholar

18 

Koss KJ and Gunnar MR: Annual research review: Early adversity, the hypothalamic-pituitary-adrenocortical axis, and child psychopathology. J Child Psychol Psychiatry. 59:327–346. 2018.PubMed/NCBI View Article : Google Scholar

19 

Szczepek AJ, Dietz GPH, Reich U, Hegend O, Olze H and Mazurek B: Differences in Stress-induced modulation of the auditory system between Wistar and Lewis rats. Front Neurosci. 12(828)2018.PubMed/NCBI View Article : Google Scholar

20 

Dmitrieva NO, Almeida DM, Dmitrieva J, Loken E and Pieper CF: A day-centered approach to modeling cortisol: Diurnal cortisol profiles and their associations among U.S. adults. Psychoneuroendocrinology. 38:2354–2365. 2013.PubMed/NCBI View Article : Google Scholar

21 

Skobowiat C, Nejati R, Lu L, Williams RW and Slominski AT: Genetic variation of the cutaneous HPA axis: An analysis of UVB-induced differential responses. Gene. 530:1–7. 2013.PubMed/NCBI View Article : Google Scholar

22 

Chen X, Gianferante D, Hanlin L, Fiksdal A, Breines JG, Thoma MV and Rohleder N: HPA-axis and inflammatory reactivity to acute stress is related with basal HPA-axis activity. Psychoneuroendocrinology. 78:168–176. 2017.PubMed/NCBI View Article : Google Scholar

23 

Chauhan NR, Kapoor M, Prabha Singh L, Gupta RK, Chand MR, Tulsawani R, Nanda S and Bala SS: Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation. Neuroscience. 358:79–92. 2017.PubMed/NCBI View Article : Google Scholar

24 

Phadke D, Beller JP and Tribble C: The disparate effects of epinephrine and norepinephrine on hyperglycemia in cardiovascular surgery. Heart Surg Forum. 21:E522–E526. 2018.PubMed/NCBI View Article : Google Scholar

25 

Quas JA, Yim IS, Oberlander TF, Nordstokke D, Essex MJ, Armstrong JM, Bush N, Obradović J and Boyce WT: The symphonic structure of childhood stress reactivity: Patterns of sympathetic, parasympathetic, and adrenocortical responses to psychological challenge. Dev Psychopathol. 26:963–982. 2014.PubMed/NCBI View Article : Google Scholar

26 

Qiu Dingjie. Study on the variation of liver hsp70 expression and mRNA abundance by heat stress in rats Fujian Agriculture and Forestry University, 2010.

27 

Zininga T, Ramatsui L and Shonhai A: Heat shock proteins as immunomodulants. Molecules. 23(2846)2018.PubMed/NCBI View Article : Google Scholar

28 

Pockley AG and Henderson B: Extracellular cell stress (heat shock) proteins-immune responses and disease: An overview. Philos Trans R Soc Lond B Biol Sci. 373(20160522)2018.PubMed/NCBI View Article : Google Scholar

29 

Chen H, Wu Y, Zhang Y, Jin L, Luo L, Xue B, Lu C, Zhang X and Yin Z: Hsp70 inhibits lipopolysaccharide-induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination. J FEBS Lett. 580:3145–3152. 2006.PubMed/NCBI View Article : Google Scholar

30 

Morimoto R, Sarge KD and Abravaya K: Transcriptional regulation of heat shock genes. A paradigm for inducible genomic responses. J Biol Chem. 267:21987–21990. 1992.PubMed/NCBI

31 

Richter-Landsberg C and Goldbaum O: Stress proteins in neural cells: Functional roles in health and disease. Cell Mol Life Sci. 60:337–349. 2003.PubMed/NCBI View Article : Google Scholar

32 

Zhang W, Yang H, Zhu L, Luo Y, Nie L and Li G: Role of EGFR/ErbB2 and PI3K/AKT/e-NOS in Lycium barbarum polysaccharides ameliorating endothelial dysfunction induced by oxidative stress. Am J Chin Med. 47:1523–1539. 2019.PubMed/NCBI View Article : Google Scholar

33 

He S, Yu Q, He Y, Hu R, Xia S and He J: Dietary resveratrol supplementation inhibits heat stress-induced high-activated innate immunity and inflammatory response in spleen of yellow-feather broilers. Poult Sci. 98:6378–6387. 2019.PubMed/NCBI View Article : Google Scholar

34 

Liu J, Zhao H, Wang Y, Shao Y, Zhang L and Xing M: Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus. Int Immunopharmacol. 64:60–68. 2018.PubMed/NCBI View Article : Google Scholar

35 

Xinlong C: Apoptosis of rat thymus cells induced by heat injury j. Foreign Med. 19:233–234. 1998.

36 

Xu J, Tang S, Yin B, Sun J, Song E and Bao E: Correction to: Co-enzyme Q10 and acetyl salicylic acid enhance Hsp70 expression in primary chicken myocardial cells to protect the cells during heat stress. Mol Cell Biochem. 461:213–214. 2019.PubMed/NCBI View Article : Google Scholar

37 

Xingguo W: Role of hypothalamic npy in chronic stress-induced depression Nanchang University, 2013.

38 

Ping W: Patterns of crh, crhr1 and crhr2 gene expression in brain and pituitary of rats induced by hypoxia Zhejiang University, 2011.

39 

Farzi A, Reichmann F and Holzer P: The homeostatic role of neuropeptide Y in immune function and its impact on mood and behaviour. Acta Physiol (Oxf). 213:603–627. 2015.PubMed/NCBI View Article : Google Scholar

40 

In lewicz DP, Hill SR, Jav JL, West CH, Zavosh AS and Sipols AJ: Effect of recurrent yohimbine on immediate and post-hoc behaviors, stress hormones, and energy homeostatic parameters. Physiol Behay. 129:186–193. 2014.PubMed/NCBI View Article : Google Scholar

41 

Zhou Y, Kruyer A and Ho A: Cocaine place conditioning increases pro-opiomelanocortin gene expression in rat hypothalamus. Neurosci Lett. 530:59–63. 2012.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhao N, Mu L, Chang X, Zhu L, Geng Y and Li G: Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats. Biomed Rep 13: 39, 2020.
APA
Zhao, N., Mu, L., Chang, X., Zhu, L., Geng, Y., & Li, G. (2020). Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats. Biomedical Reports, 13, 39. https://doi.org/10.3892/br.2020.1346
MLA
Zhao, N., Mu, L., Chang, X., Zhu, L., Geng, Y., Li, G."Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats". Biomedical Reports 13.5 (2020): 39.
Chicago
Zhao, N., Mu, L., Chang, X., Zhu, L., Geng, Y., Li, G."Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats". Biomedical Reports 13, no. 5 (2020): 39. https://doi.org/10.3892/br.2020.1346
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao N, Mu L, Chang X, Zhu L, Geng Y and Li G: Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats. Biomed Rep 13: 39, 2020.
APA
Zhao, N., Mu, L., Chang, X., Zhu, L., Geng, Y., & Li, G. (2020). Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats. Biomedical Reports, 13, 39. https://doi.org/10.3892/br.2020.1346
MLA
Zhao, N., Mu, L., Chang, X., Zhu, L., Geng, Y., Li, G."Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats". Biomedical Reports 13.5 (2020): 39.
Chicago
Zhao, N., Mu, L., Chang, X., Zhu, L., Geng, Y., Li, G."Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats". Biomedical Reports 13, no. 5 (2020): 39. https://doi.org/10.3892/br.2020.1346
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