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Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury

  • Authors:
    • Miaomiao Zhao
    • Juan Xie
    • Haorui Shen
    • Xiaoxiao Wang
    • Qiuling Wu
    • Linghui Xia
  • View Affiliations / Copyright

    Affiliations: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300000, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 8
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    Published online on: November 6, 2020
       https://doi.org/10.3892/br.2020.1384
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Abstract

Hepatic veno‑occlusive disease (VOD) is a life‑threatening complication of hematopoietic stem cell transplantation, which urgently requires effective prevention and treatment. Endothelial damage is recognized as the first event in patients with hepatic VOD. However, the mechanism by which endothelial injury induces thrombosis in hepatic VOD is still not clear. In the present study, monocrotaline (MCT) was used to induce endothelial cell injury in EA.hy926 cells to imitate in vitro hepatic VOD. MCT significantly increased apoptosis in EA.hy926 endothelial cells and the secretion of endothelial microparticles (EMPs) which can be used to reflect the level of endothelial injury. Additionally, MCT significantly enhanced the expression of soluble tissue factor (TF) and EMP‑bound TF protein, suggesting that EMPs may participate in the development of hepatic VOD by regulating coagulation. Ginsenoside Rb1, a major constituent and effective ingredient of Panax ginseng, was found to significantly decrease MCT‑induced endothelial injury and release of EMPs. Moreover, Ginsenoside Rb1 decreased soluble TF released by EA.hy926 cells and EMP‑bound TF protein induced by MCT. These data suggest that ginsenoside Rb1 may serve as a potent prophylactic and/or as a treatment of hepatic VOD by protecting endothelial cells and preventing microthrombosis induced by endothelial injury.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao M, Xie J, Shen H, Wang X, Wu Q and Xia L: Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury. Biomed Rep 14: 8, 2021.
APA
Zhao, M., Xie, J., Shen, H., Wang, X., Wu, Q., & Xia, L. (2021). Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury. Biomedical Reports, 14, 8. https://doi.org/10.3892/br.2020.1384
MLA
Zhao, M., Xie, J., Shen, H., Wang, X., Wu, Q., Xia, L."Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury". Biomedical Reports 14.1 (2021): 8.
Chicago
Zhao, M., Xie, J., Shen, H., Wang, X., Wu, Q., Xia, L."Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury". Biomedical Reports 14, no. 1 (2021): 8. https://doi.org/10.3892/br.2020.1384
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao M, Xie J, Shen H, Wang X, Wu Q and Xia L: Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury. Biomed Rep 14: 8, 2021.
APA
Zhao, M., Xie, J., Shen, H., Wang, X., Wu, Q., & Xia, L. (2021). Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury. Biomedical Reports, 14, 8. https://doi.org/10.3892/br.2020.1384
MLA
Zhao, M., Xie, J., Shen, H., Wang, X., Wu, Q., Xia, L."Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury". Biomedical Reports 14.1 (2021): 8.
Chicago
Zhao, M., Xie, J., Shen, H., Wang, X., Wu, Q., Xia, L."Role of endothelial‑microparticles and the tissue factor pathway in ginsenoside Rb1‑mediated prevention of umbilical vein endothelial cell injury". Biomedical Reports 14, no. 1 (2021): 8. https://doi.org/10.3892/br.2020.1384
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