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Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium

  • Authors:
    • Jae-Yong Tae
    • Hyunjin Lee
    • Hyuna Lee
    • Youngmin Song
    • Jun-Beom Park
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
    Copyright: © Tae et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 9
    |
    Published online on: November 11, 2020
       https://doi.org/10.3892/br.2020.1385
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Abstract

The aim of the present study was to evaluate the morphology, cellular viability and stem cell marker expression of three‑dimensional cultures of bone marrow and gingiva‑derived stem cells in different ratios. Stem cell spheroids were made with bone marrow and gingiva‑derived stem cells using ratios of 6:0 (Group 1), 4:2 (Group 2), 3:3 (Group 3), 2:4 (Group 4) and 0:6 (Group 5), respectively. The viability of cell spheroids was analyzed using a Live/Dead kit assay and a Cell Counting Kit‑8 assay. Total RNA extraction and reverse transcription‑quantitative PCR were performed to detect the mRNA expression levels of Nanog and β‑actin in each group. Stem cell spheroids were well formed in silicone elastomer‑based concave microwells with different ratios of bone marrow and gingiva‑derived stem cells. The shape of the spheroids and their viability were maintained throughout the entirety of the experimental procedure. Statistically significant increases in spheroid diameters were noted in Groups 4 and 5 on day 1 when compared with Group 1 on day 1. There was a significant increase in the cell viability values seen in Group 3 on day 1 when compared with Group 1 on day 1. Highest levels of Nanog expression was seen in Group 3 on day 10, but the increase was not significant when compared with Group 1 on day 1. Co‑culturing with higher ratios of gingiva‑derived stem cells produced stem cell spheroids with larger diameters and increased cellular viability. This co‑culture technique may be used in stem cell therapy with allogenic stem cell transplantation.
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1 

Pasca SP: The rise of three-dimensional human brain cultures. Nature. 553:437–445. 2018.PubMed/NCBI View Article : Google Scholar

2 

Mazza G, Al-Akkad W, Rombouts K and Pinzani M: Liver tissue engineering: From implantable tissue to whole organ engineering. Hepatol Commun. 2:131–141. 2017.PubMed/NCBI View Article : Google Scholar

3 

Ong SM, Zhang C, Toh YC, Kim SH, Foo HL, Tan CH, van Noort D, Park S and Yu H: A gel-free 3D microfluidic cell culture system. Biomaterials. 29:3237–3244. 2008.PubMed/NCBI View Article : Google Scholar

4 

Pamies D, Block K, Lau P, Gribaldo L, Pardo CA, Barreras P, Smirnova L, Wiersma D, Zhao L, Harris G, et al: Rotenone exerts developmental neurotoxicity in a human brain spheroid model. Toxicol Appl Pharmacol. 354:101–114. 2018.PubMed/NCBI View Article : Google Scholar

5 

Lee GH, Suh Y and Park JY: A paired bead and magnet array for molding microwells with variable concave geometries. J Vis Exp. 28(55548)2018.PubMed/NCBI View Article : Google Scholar

6 

Bauman E, Feijao T, Carvalho DTO, Granja PL and Barrias CC: Xeno-free pre-vascularized spheroids for therapeutic applications. Sci Rep. 8(230)2018.PubMed/NCBI View Article : Google Scholar

7 

Redondo-Castro E, Cunningham CJ, Miller J, Brown H, Allan SM and Pinteaux E: Changes in the secretome of tri-dimensional spheroid-cultured human mesenchymal stem cells in vitro by interleukin-1 priming. Stem Cell Res Ther. 9(11)2018.PubMed/NCBI View Article : Google Scholar

8 

Lee SI, Yeo SI, Kim BB, Ko Y and Park JB: Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests. Biomed Rep. 4:97–101. 2016.PubMed/NCBI View Article : Google Scholar

9 

Cen LP, Ng TK, Liang JJ, Zhuang X, Yao X, Yam GHF, Chen H, Cheung HS, Zhang M and Pang CP: Human periodontal ligament-derived stem cells promote retinal ganglion cell survival and axon regeneration after optic nerve injury. Stem Cells. 36:844–856. 2018.PubMed/NCBI View Article : Google Scholar

10 

Taniguchi D, Matsumoto K, Tsuchiya T, Machino R, Takeoka Y, Elgalad A, Gunge K, Takagi K, Taura Y, Hatachi G, et al: Scaffold-free trachea regeneration by tissue engineering with bio-3D printing. Interact Cardiovasc Thorac Surg. 26:745–752. 2018.PubMed/NCBI View Article : Google Scholar

11 

Oberringer M, Bubel M, Jennewein M, Guthörl S, Morsch T, Bachmann S, Metzger W and Pohlemann T: The role of adipose-derived stem cells in a self-organizing 3D model with regard to human soft tissue healing. Mol Cell Biochem. 445:195–210. 2018.PubMed/NCBI View Article : Google Scholar

12 

Tae JY, Lee SI, Ko Y and Park JB: Enhanced osteogenic differentiation potential of stem-cell spheroids created from a coculture of stem cells and endothelial cells. Implant Dent. 26:922–928. 2017.PubMed/NCBI View Article : Google Scholar

13 

Tae JY, Lee H, Lee H, Ko Y and Park JB: Osteogenic potential of cell spheroids composed of varying ratios of gingiva-derived and bone marrow stem cells using concave microwells. Exp Ther Med. 16:2287–2294. 2018.PubMed/NCBI View Article : Google Scholar

14 

Jin SH, Lee JE, Yun JH, Kim I, Ko Y and Park JB: Isolation and characterization of human mesenchymal stem cells from gingival connective tissue. J Periodontal Res. 50:461–467. 2015.PubMed/NCBI View Article : Google Scholar

15 

Jeong CH, Kim SM, Lim JY, Ryu CH, Jun JA and Jeun SS: Mesenchymal stem cells expressing brain-derived neurotrophic factor enhance endogenous neurogenesis in an ischemic stroke model. Biomed Res Int. 2014(129145)2014.PubMed/NCBI View Article : Google Scholar

16 

World Medical Association. World medical association declaration of helsinki: Ethical principles for medical research involving human subjects. JAMA. 310:2191–2194. 2013.PubMed/NCBI View Article : Google Scholar

17 

Kang SH, Park JB, Kim I, Lee W and Kim H: Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold. J Periodontal Implant Sci. 49:258–267. 2019.PubMed/NCBI View Article : Google Scholar

18 

Tae JY, Ko Y and Park JB: Evaluation of fibroblast growth factor-2 on the proliferation of osteogenic potential and protein expression of stem cell spheroids composed of stem cells derived from bone marrow. Exp Ther Med. 18:326–331. 2019.PubMed/NCBI View Article : Google Scholar

19 

Lee H, Min SK, Song Y, Park YH and Park JB: Bone morphogenetic protein-7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva-derived stem cells. Exp Ther Med. 18:2867–2876. 2019.PubMed/NCBI View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

21 

Kook YM, Kim H, Kim S, Heo CY, Park MH, Lee K and Koh WG: Promotion of vascular morphogenesis of endothelial cells co-cultured with human adipose-derived mesenchymal stem cells using polycaprolactone/gelatin nanofibrous scaffolds. Nanomaterials (Basel). 8(117)2018.PubMed/NCBI View Article : Google Scholar

22 

Oliveira MN, Pillat MM, Motaln H, Ulrich H and Lah TT: Kinin-B1 receptor stimulation promotes invasion and is involved in cell-cell interaction of co-cultured glioblastoma and mesenchymal stem cells. Sci Rep. 8(1299)2018.PubMed/NCBI View Article : Google Scholar

23 

Lee SI, Ko Y and Park JB: Evaluation of the maintenance of stemness, viability, and differentiation potential of gingiva-derived stem-cell spheroids. Exp Ther Med. 13:1757–1764. 2017.PubMed/NCBI View Article : Google Scholar

24 

Ha DH, Pathak S, Yong CS, Kim JO, Jeong JH and Park JB: Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Sci Rep. 6(34910)2016.PubMed/NCBI View Article : Google Scholar

25 

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K and Yamanaka S: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131:861–872. 2007.PubMed/NCBI View Article : Google Scholar

26 

Zhou X, Zhou YP, Huang GR, Gong BL, Yang B, Zhang DX, Hu P and Xu SR: Expression of the stem cell marker, Nanog, in human endometrial adenocarcinoma. Int J Gynecol Pathol. 30:262–270. 2011.PubMed/NCBI View Article : Google Scholar

27 

Lee H, Min SK and Park JB: Effects of demographic factors on adipogenic and chondrogenic differentiation in bone marrow-derived stem cells. Exp Ther Med. 17:3548–3554. 2019.PubMed/NCBI View Article : Google Scholar

28 

Khoshlahni N, Sagha M, Mirzapour T, Zarif MN and Mohammadzadeh-Vardin M: Iron depletion with deferoxamine protects bone marrow-derived mesenchymal stem cells against oxidative stress-induced apoptosis. Cell Stress Chaperones. 25:1059–1069. 2020.PubMed/NCBI View Article : Google Scholar

29 

Dustin ML: Visualization of cell-cell interaction contacts: Synapses and kinapses. Self Nonself. 2:85–97. 2011.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Tae J, Lee H, Lee H, Song Y and Park J: Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium. Biomed Rep 14: 9, 2021.
APA
Tae, J., Lee, H., Lee, H., Song, Y., & Park, J. (2021). Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium. Biomedical Reports, 14, 9. https://doi.org/10.3892/br.2020.1385
MLA
Tae, J., Lee, H., Lee, H., Song, Y., Park, J."Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium". Biomedical Reports 14.1 (2021): 9.
Chicago
Tae, J., Lee, H., Lee, H., Song, Y., Park, J."Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium". Biomedical Reports 14, no. 1 (2021): 9. https://doi.org/10.3892/br.2020.1385
Copy and paste a formatted citation
x
Spandidos Publications style
Tae J, Lee H, Lee H, Song Y and Park J: Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium. Biomed Rep 14: 9, 2021.
APA
Tae, J., Lee, H., Lee, H., Song, Y., & Park, J. (2021). Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium. Biomedical Reports, 14, 9. https://doi.org/10.3892/br.2020.1385
MLA
Tae, J., Lee, H., Lee, H., Song, Y., Park, J."Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium". Biomedical Reports 14.1 (2021): 9.
Chicago
Tae, J., Lee, H., Lee, H., Song, Y., Park, J."Morphological stability, cellular viability and stem cell marker expression of three‑dimensional cultures of stem cells from bone marrow and periodontium". Biomedical Reports 14, no. 1 (2021): 9. https://doi.org/10.3892/br.2020.1385
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