Open Access

Anti‑diabetic effect of the lupinalbin A compound isolated from Apios americana: In vitro analysis and molecular docking study

  • Authors:
    • Hyo-Young Kim
    • Jang Hoon Kim
    • Hye Gwang Jeong
    • Chang Hyun Jin
  • View Affiliations

  • Published online on: February 26, 2021     https://doi.org/10.3892/br.2021.1415
  • Article Number: 39
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dipeptidyl peptidase 4 (DPP4) and α‑glucosidase inhibitors have been developed as anti‑diabetic agents for the treatment of diabetes mellitus. In the present study, the anti‑diabetic effects of the lupinalbin A compound isolated from Apios americana was investigated by measuring its inhibitory activity against DPP4 and α‑glucosidase. To detect the inhibitory effect of lupinalbin A, DPP4 and α‑glucosidase assays were performed in vitro. Molecular docking analysis was performed using AutoDock 4.2. The IC50 values of lupinalbin A against DPP4 and α‑glucosidase were 45.2 and 53.4 µM, respectively. Analysis of the enzyme kinetics revealed that lupinalbin A interacted with the active site of DPP4 in a competitive manner, with an inhibition constant (Ki) value of 35.1±2.0 µM, whereas the lupinalbin A interaction with α‑glucosidase was non‑competitive, with a Ki value of 45.0 µM. Molecular docking analysis revealed a binding pose between the DPP4 enzyme and lupinalbin A. Taken together, these data suggest lupinalbin A is more effective against DPP4 than α‑glucosidase, with regard to its anti‑diabetic effects.
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April-2021
Volume 14 Issue 4

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Spandidos Publications style
Kim H, Kim J, Jeong H and Jin C: Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study. Biomed Rep 14: 39, 2021
APA
Kim, H., Kim, J., Jeong, H., & Jin, C. (2021). Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study. Biomedical Reports, 14, 39. https://doi.org/10.3892/br.2021.1415
MLA
Kim, H., Kim, J., Jeong, H., Jin, C."Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study". Biomedical Reports 14.4 (2021): 39.
Chicago
Kim, H., Kim, J., Jeong, H., Jin, C."Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study". Biomedical Reports 14, no. 4 (2021): 39. https://doi.org/10.3892/br.2021.1415