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Anti‑diabetic effect of the lupinalbin A compound isolated from Apios americana: In vitro analysis and molecular docking study

  • Authors:
    • Hyo-Young Kim
    • Jang Hoon Kim
    • Hye Gwang Jeong
    • Chang Hyun Jin
  • View Affiliations / Copyright

    Affiliations: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup‑si, Jeollabuk‑do 56212, Republic of Korea, College of Pharmacy, Chungnam National University, Daejeon, Chungcheongnam‑do 34134, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 39
    |
    Published online on: February 26, 2021
       https://doi.org/10.3892/br.2021.1415
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Abstract

Dipeptidyl peptidase 4 (DPP4) and α‑glucosidase inhibitors have been developed as anti‑diabetic agents for the treatment of diabetes mellitus. In the present study, the anti‑diabetic effects of the lupinalbin A compound isolated from Apios americana was investigated by measuring its inhibitory activity against DPP4 and α‑glucosidase. To detect the inhibitory effect of lupinalbin A, DPP4 and α‑glucosidase assays were performed in vitro. Molecular docking analysis was performed using AutoDock 4.2. The IC50 values of lupinalbin A against DPP4 and α‑glucosidase were 45.2 and 53.4 µM, respectively. Analysis of the enzyme kinetics revealed that lupinalbin A interacted with the active site of DPP4 in a competitive manner, with an inhibition constant (Ki) value of 35.1±2.0 µM, whereas the lupinalbin A interaction with α‑glucosidase was non‑competitive, with a Ki value of 45.0 µM. Molecular docking analysis revealed a binding pose between the DPP4 enzyme and lupinalbin A. Taken together, these data suggest lupinalbin A is more effective against DPP4 than α‑glucosidase, with regard to its anti‑diabetic effects.
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1 

Wild S, Roglic G, Green A, Sicree R and King H: Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 27:1047–1053. 2004.PubMed/NCBI View Article : Google Scholar

2 

Kim SM, Lee JS, Lee J, Na JK, Han JH, Yoon DK, Baik SH, Choi DS and Choi KM: Prevalence of diabetes and impaired fasting glucose in Korea: Korean National Health and Nutrition Survey 2001. Diabetes Care. 29:226–231. 2006.PubMed/NCBI View Article : Google Scholar

3 

DeFronzo RA: Pathogenesis of type 2 diabetes mellitus. Med Clin North Am. 88:787–835, ix. 2004.PubMed/NCBI View Article : Google Scholar

4 

Zhao BT, Le DD, Nguyen PH, Ali MY, Choi JS, Min BS, Shin HM, Rhee HI and Woo MH: PTP1B, α-glucosidase, and DPP-IV inhibitory effects for chromene derivatives from the leaves of Smilax china L. Chem Biol Interact. 253:27–37. 2016.PubMed/NCBI View Article : Google Scholar

5 

Li ZP, Song YH, Uddin Z, Wang Y and Park KH: Inhibition of protein tyrosine phosphatase 1B (PTP1B) and α-glucosidase by xanthones from Cratoxylum cochinchinense, and their kinetic characterization. Bioorg Med Chem. 26:737–746. 2018.PubMed/NCBI View Article : Google Scholar

6 

Yazbeck R, Howarth GS and Abbott CA: Dipeptidyl peptidase inhibitors, an emerging drug class for inflammatory disease? Trends Pharmacol Sci. 30:600–607. 2009.PubMed/NCBI View Article : Google Scholar

7 

Hopsu-Havu VK and Glenner GG: A new dipeptide naphthylamidase hydrolyzing glycyl-prolyl-beta-naphthylamide. Histochemie. 7:197–201. 1966.PubMed/NCBI View Article : Google Scholar

8 

Gorrell MD, Gysbers V and McCaughan GW: CD26: A multifunctional integral membrane and secreted protein of activated lymphocytes. Scand J Immunol. 54:249–264. 2001.PubMed/NCBI View Article : Google Scholar

9 

Morimoto C and Schlossman SF: The structure and function of CD26 in the T-cell immune response. Immunol Rev. 161:55–70. 1998.PubMed/NCBI View Article : Google Scholar

10 

Drucker DJ: Dipeptidyl peptidase-4 inhibition and the treatment of type 2 diabetes: Preclinical biology and mechanisms of action. Diabetes Care. 30:1335–1343. 2007.PubMed/NCBI View Article : Google Scholar

11 

Mulvihill EE and Drucker DJ: Pharmacology, physiology, and mechanisms of action of dipeptidyl peptidase-4 inhibitors. Endocr Rev. 35:992–1019. 2014.PubMed/NCBI View Article : Google Scholar

12 

Wilson PW, Pichardo FJ, Liuzzo JA, Blackmon WJ and Reynolds BD: Amino acids in the american groundnut (Apios americana). J Food Sci. 52:224–225. 1987.

13 

Ichige M, Fukuda E, Miida S, Hattan J, Misawa N, Saito S, Fujimaki T, Imoto M and Shindo K: Novel isoflavone glucosides in groundnut (Apios americana Medik) and their antiandrogenic activities. J Agric Food Chem. 61:2183–2187. 2013.PubMed/NCBI View Article : Google Scholar

14 

Kaneta H, Koda M, Saito S, Imoto M, Kawada M, Yamazaki Y, Momose I and Shindo K: Biological activities of unique isoflavones prepared from Apios americana Medik. Biosci Biotechnol Biochem. 80:774–778. 2016.PubMed/NCBI View Article : Google Scholar

15 

Kim JH, Kim HY, Kang SY, Kim YH and Jin CH: Soluble epoxide hydrolase inhibitory activity of components isolated from Apios americana Medik. Molecules. 22(1432)2017.PubMed/NCBI View Article : Google Scholar

16 

Kim JH, Kim HY, Kang SY, Kim JB, Kim YH and Jin CH: Chemical constituents from Apios americana and their inhibitory activity on tyrosinase. Molecules. 23(232)2018.PubMed/NCBI View Article : Google Scholar

17 

Iwai K and Matsue H: Ingestion of Apios americana Medikus tuber suppresses blood pressure and improves plasma lipids in spontaneously hypertensive rats. Nutr Res. 27:218–224. 2007.

18 

Gilbert ER and Liu D: Anti-diabetic functions of soy isoflavone genistein: Mechanisms underlying its effects on pancreatic β-cell function. Food Funct. 4:200–212. 2013.PubMed/NCBI View Article : Google Scholar

19 

Kim HY, Kim JH, So Y, Kang SY, Jeong HG and Jin CH: Anti-inflammatory effect of lupinalbin A isolated from Apios americana on lipopolysaccharide-treated RAW264.7 cells. Molecules. 23(583)2018.PubMed/NCBI View Article : Google Scholar

20 

Bai Y, Xu Y, Chang J, Wang X, Zhao Y and Yu Z: Bioactives from stems and leaves of mung beans (Vigna radiata L.). J Funct Foods. 25:314–322. 2016.

21 

Yang D, Xie H, Jiang Y and Wei X: Phenolics from strawberry cv. Falandi and their antioxidant and α-glucosidase inhibitory activities. Food Chem. 194:857–863. 2016.PubMed/NCBI View Article : Google Scholar

22 

Kim JH, Ryu YB, Lee WS and Kim YH: Neuraminidase inhibitory activities of quaternary isoquinoline alkaloids from Corydalis turtschaninovii rhizome. Bioorg Med Chem. 22:6047–6052. 2014.PubMed/NCBI View Article : Google Scholar

23 

Meng XY, Zhang HX, Mezei M and Cui M: Molecular docking: A powerful approach for structure-based drug discovery. Curr Comput Aided Drug Des. 7:146–157. 2011.PubMed/NCBI View Article : Google Scholar

24 

de Ruyck J, Brysbaert G, Blossey R and Lensink MF: Molecular docking as a popular tool in drug design, an in silico travel. Adv Appl Bioinform Chem. 9:1–11. 2016.PubMed/NCBI View Article : Google Scholar

25 

Heitz MP and Rupp JW: Determining mushroom tyrosinase inhibition by imidazolium ionic liquids: A spectroscopic and molecular docking study. Int J Biol Macromol. 107 (Pt B):1971–1981. 2018.PubMed/NCBI View Article : Google Scholar

26 

Seong SH, Ali MY, Kim HR, Jung HA and Choi JS: BACE1 inhibitory activity and molecular docking analysis of meroterpenoids from Sargassum serratifolium. Bioorg Med Chem. 25:3964–3970. 2017.PubMed/NCBI View Article : Google Scholar

27 

Fan J, Johnson MH, Lila MA, Yousef G and de Mejia EG: Berry and citrus phenolic compounds inhibit dipeptidyl peptidase IV: Implications in diabetes management. Evid Based Complement Alternat Med. 2013(479505)2013.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Kim J, Jeong H and Jin C: Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study. Biomed Rep 14: 39, 2021.
APA
Kim, H., Kim, J., Jeong, H., & Jin, C. (2021). Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study. Biomedical Reports, 14, 39. https://doi.org/10.3892/br.2021.1415
MLA
Kim, H., Kim, J., Jeong, H., Jin, C."Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study". Biomedical Reports 14.4 (2021): 39.
Chicago
Kim, H., Kim, J., Jeong, H., Jin, C."Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study". Biomedical Reports 14, no. 4 (2021): 39. https://doi.org/10.3892/br.2021.1415
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Kim J, Jeong H and Jin C: Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study. Biomed Rep 14: 39, 2021.
APA
Kim, H., Kim, J., Jeong, H., & Jin, C. (2021). Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study. Biomedical Reports, 14, 39. https://doi.org/10.3892/br.2021.1415
MLA
Kim, H., Kim, J., Jeong, H., Jin, C."Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study". Biomedical Reports 14.4 (2021): 39.
Chicago
Kim, H., Kim, J., Jeong, H., Jin, C."Anti‑diabetic effect of the lupinalbin A compound isolated from <em>Apios americana</em>: <em>In vitro</em> analysis and molecular docking study". Biomedical Reports 14, no. 4 (2021): 39. https://doi.org/10.3892/br.2021.1415
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