Leptin and the GA genotype of rs2167270 of the <em>LEP</em> gene increase the risk of prediabetes

Aljanabi,Mukhallad A.; Alfaqih,Mahmoud A.; Khanfar,Mariam; Amarin,Zouhair O.; Elsalem,Lina; Saadeh,Rami; Al‑Μughales,Faheem

doi:10.3892/br.2021.1420

Leptin and the GA genotype of rs2167270 of the LEP gene increase the risk of prediabetes

Authors:
- Mukhallad A. Aljanabi
- Mahmoud A. Alfaqih
- Mariam Khanfar
- Zouhair O. Amarin
- Lina Elsalem
- Rami Saadeh
- Faheem Al‑Μughales
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Affiliations: Department of Physiology and Biochemistry, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Obstetrics and Gynecology, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Pharmacology, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Community Medicine and Public Health, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan
Published online on: March 9, 2021 https://doi.org/10.3892/br.2021.1420
Article Number: 44

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Abstract

Prediabetes is a precursor stage that frequently develops to definitive type 2 diabetes mellitus (T2DM). Therefore, identifying individuals with prediabetes can allow for early intervention measures that delay or prevent disease progression to T2DM. Several biochemical changes appear to be associated with prediabetes, including an increase in the serum levels of leptin. In Jordan, this association has not been previously investigated. In the present study, the serum levels of leptin were measured in 122 prediabetes subjects and 122 controls. Furthermore, the genotypes of three single nucleotide polymorphisms in the LEP gene (rs7799039, rs2167270 and rs791620) were investigated for their association with prediabetes using PCR‑restriction fragment length polymorphism. The results revealed a significant increase in serum leptin levels in the prediabetes group. It was also shown that the GA genotype and the A allele of rs2167270 were significantly associated with an increased risk of prediabetes (P<0.05). These findings were shown to be independent of body mass index, waist circumference and serum glucose levels. To the best of our knowledge, the present study is the first in Jordan to have reported an association between serum leptin levels and the GA genotype of rs2167270 with an increased risk of prediabetes, identified both in the univariate and multivariate models.

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