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Article

Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12

  • Authors:
    • Matthew Krivacka Kay
    • Jian Zhang
    • Mahua Choudhury
  • View Affiliations / Copyright

    Affiliations: Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, Texas, TX 77843, USA
  • Article Number: 50
    |
    Published online on: April 1, 2021
       https://doi.org/10.3892/br.2021.1426
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Abstract

The long non coding RNA deleted in leukemia 2 gene (Dleu2) has recently been demonstrated to be an active player in the progression of several types of cancer, including hepatocellular carcinoma. Dleu2 may serve a role in modulating the downstream effects‑mediated by alternative splicing of its multiple exons. However, the proportional expression of these alternative splicing populations of the Dleu2 exons is currently unknown. To determine how Dleu2 could be affected by alternative splicing, a series of alternative splicing primer sets were designed to investigate which transcripts were preferentially activated when Dleu2 was targeted for downregulation or upregulation. A specific Dleu2 small interfering RNA that targeted an exon upstream of the tumor suppressor microRNA site significantly knocked down Dleu2 expression across all the primer sets used, which targeted 13 different alternative splicing transcripts over 5 different promoter sites in the mouse liver cell line, AML‑12. Similarly, 50 µM Resveratrol led to significant upregulation of Dleu2 in 11 alternative splicing transcripts. These results show that Dleu2 is capable of successful modulation across alternative splicing transcripts that can be screened, and also that Resveratrol can be a potential nutraceutical, which may potentially lead to novel approaches in the use of lncRNA Dleu2 for diagnostics and regulation.
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Copy and paste a formatted citation
Spandidos Publications style
Kay MK, Zhang J and Choudhury M: Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12. Biomed Rep 14: 50, 2021.
APA
Kay, M.K., Zhang, J., & Choudhury, M. (2021). Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12. Biomedical Reports, 14, 50. https://doi.org/10.3892/br.2021.1426
MLA
Kay, M. K., Zhang, J., Choudhury, M."Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12". Biomedical Reports 14.6 (2021): 50.
Chicago
Kay, M. K., Zhang, J., Choudhury, M."Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12". Biomedical Reports 14, no. 6 (2021): 50. https://doi.org/10.3892/br.2021.1426
Copy and paste a formatted citation
x
Spandidos Publications style
Kay MK, Zhang J and Choudhury M: Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12. Biomed Rep 14: 50, 2021.
APA
Kay, M.K., Zhang, J., & Choudhury, M. (2021). Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12. Biomedical Reports, 14, 50. https://doi.org/10.3892/br.2021.1426
MLA
Kay, M. K., Zhang, J., Choudhury, M."Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12". Biomedical Reports 14.6 (2021): 50.
Chicago
Kay, M. K., Zhang, J., Choudhury, M."Screening for alternative splicing of lncRNA Dleu2 in the mouse liver cell line AML-12". Biomedical Reports 14, no. 6 (2021): 50. https://doi.org/10.3892/br.2021.1426
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