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Article

IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients

  • Authors:
    • Amer Imraish
    • Tuqa Abu‑Thiab
    • Malek Zihlif
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, School of Science, The University of Jordan, Amman, Levant 11942, Jordan, Department of Pharmacology, School of Medicine, The University of Jordan, Amman, Levant 11942, Jordan
  • Article Number: 55
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    Published online on: April 13, 2021
       https://doi.org/10.3892/br.2021.1431
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Abstract

Immunoglobulin E (IgE) serves a crucial role in the pathogenesis of several allergic disorders, and elevated levels of total serum IgE have been associated with asthma. IgE is responsible for the release of several asthma‑associated inflammatory mediators from mast cells, such as histamine and prostaglandins. The aim of the present study was to assess the association of interleukin (IL)‑13 single nucleotide polymorphism (SNP) rs20541 and forkhead box O3a (FOXO3a) SNP rs13217795 with IgE levels in asthmatic patients and a healthy control group. Genetic polymorphism analysis of SNPs was performed using PCR/restriction fragment length polymorphism. Total serum IgE levels were measured using an ELISA kit. Genotypes were grouped into three models: Co‑dominant, dominant and recessive. Major and minor alleles for IL‑13 SNP rs20541 and FOXO3a SNP rs13217795 were C and T, whereas for IL‑13, they were G and A, respectively. There was a significant association between the IL‑13 rs20541 SNP and the total IgE serum levels, in which pure minor alleles were associated with a significant reduction (~5x lower) in IgE serum levels compared with the major alleles in asthmatic subjects and to a lesser extent in the control subjects. Additionally, the FOXO3a rs13217795 SNP was associated with a significant increase in total IgE levels (~5x higher) in the asthmatic patients compared with the control subjects. In conclusion, the present study confirmed that there was a significant association between the IL‑13 SNP rs20541 and asthma, and an association between the FOXO3a SNP rs13217795 with asthma pathogenicity in Jordanian subjects.
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Copy and paste a formatted citation
Spandidos Publications style
Imraish A, Abu‑Thiab T and Zihlif M: IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients. Biomed Rep 14: 55, 2021.
APA
Imraish, A., Abu‑Thiab, T., & Zihlif, M. (2021). IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients. Biomedical Reports, 14, 55. https://doi.org/10.3892/br.2021.1431
MLA
Imraish, A., Abu‑Thiab, T., Zihlif, M."IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients". Biomedical Reports 14.6 (2021): 55.
Chicago
Imraish, A., Abu‑Thiab, T., Zihlif, M."IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients". Biomedical Reports 14, no. 6 (2021): 55. https://doi.org/10.3892/br.2021.1431
Copy and paste a formatted citation
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Spandidos Publications style
Imraish A, Abu‑Thiab T and Zihlif M: IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients. Biomed Rep 14: 55, 2021.
APA
Imraish, A., Abu‑Thiab, T., & Zihlif, M. (2021). IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients. Biomedical Reports, 14, 55. https://doi.org/10.3892/br.2021.1431
MLA
Imraish, A., Abu‑Thiab, T., Zihlif, M."IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients". Biomedical Reports 14.6 (2021): 55.
Chicago
Imraish, A., Abu‑Thiab, T., Zihlif, M."IL‑13 and FOXO3 genes polymorphisms regulate IgE levels in asthmatic patients". Biomedical Reports 14, no. 6 (2021): 55. https://doi.org/10.3892/br.2021.1431
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