Open Access

Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco‑2 cells

  • Authors:
    • Atcharaporn Ontawong
    • Acharaporn Duangjai
    • Chutima Srimaroeng
  • View Affiliations

  • Published online on: July 12, 2021     https://doi.org/10.3892/br.2021.1449
  • Article Number: 73
  • Copyright: © Ontawong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The major constituents of Coffea arabica (coffee), including caffeine, chlorogenic acid and caffeic acid, exhibit antihyperglycemic properties in in vitro and in vivo models. However, whether Coffea arabica bean extract (CBE) regulates glucose uptake activity and the underlying mechanisms involved remain unclear. The aim of the present study was to examine the effects of CBE on glucose absorption and identify the mechanisms involved using an in vitro model. The uptake of a fluorescent glucose analog into Caco‑2 colorectal adenocarcinoma cells was determined. The expression levels of sodium glucose co‑transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) were evaluated. In addition, glycoside hydrolase enzyme activity was investigated. It was observed that CBE inhibited disaccharidase enzyme activity. Furthermore, CBE exerted an inhibitory effect on intestinal glucose absorption by downregulating SGLT1‑ and GLUT2‑mediated 5' AMP‑activated protein kinase phosphorylation and suppressing hepatocyte nuclear factor 1α expression. These data suggest that CBE may attenuate glucose absorption and may have potentially beneficial antihyperglycemic effects in the body; however, the mechanisms underlying the effects of CBE must be elucidated through further investigation.
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September-2021
Volume 15 Issue 3

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Spandidos Publications style
Ontawong A, Duangjai A and Srimaroeng C: <em>Coffea arabica</em> bean extract inhibits glucose transport and disaccharidase activity in Caco‑2 cells. Biomed Rep 15: 73, 2021
APA
Ontawong, A., Duangjai, A., & Srimaroeng, C. (2021). <em>Coffea arabica</em> bean extract inhibits glucose transport and disaccharidase activity in Caco‑2 cells. Biomedical Reports, 15, 73. https://doi.org/10.3892/br.2021.1449
MLA
Ontawong, A., Duangjai, A., Srimaroeng, C."<em>Coffea arabica</em> bean extract inhibits glucose transport and disaccharidase activity in Caco‑2 cells". Biomedical Reports 15.3 (2021): 73.
Chicago
Ontawong, A., Duangjai, A., Srimaroeng, C."<em>Coffea arabica</em> bean extract inhibits glucose transport and disaccharidase activity in Caco‑2 cells". Biomedical Reports 15, no. 3 (2021): 73. https://doi.org/10.3892/br.2021.1449