Open Access

B1 repetitive sequence methylation enhances wound healing of second‑degree burns in rats

  • Authors:
    • Jiraroch Meevassana
    • Panupong Nacharoenkul
    • Jade Wititsuwannakul
    • Nakarin Kitkumthorn
    • Kevin J. Hamill
    • Apichai Angspatt
    • Apiwat Mutirangura
  • View Affiliations

  • Published online on: February 3, 2022     https://doi.org/10.3892/br.2022.1503
  • Article Number: 20
  • Copyright: © Meevassana et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The accumulation of DNA damage in burn wounds delays wound healing. DNA methylation by short interspersed nuclear element (SINE) small interfering (si)RNA prevents DNA damage and promotes cell proliferation. Therefore, SINE siRNA may be able to promote burn wound healing. Here, a SINE B1 siRNA was used to treat burn wounds in rats. Second‑degree burn wounds were introduced on the backs of rats. The rats were then divided into three groups: a B1 siRNA‑treated, saline‑treated control, and saline + calcium phosphate‑nanoparticle‑treated control group (n=15/group). The wounds were imaged on days 0, 7, 14, 21 and 28 post‑injury. The tissue sections were processed for methylation, histological and immunohistochemical examination, and scored based on the overall expression of histone H2AX phosphorylated on serine 139 (γH2AX) and 8‑hydroxy‑2'‑deoxyguanosine (8‑OHdG). Burn wound closure improved in the B1 siRNA‑treated group compared with that in the control group, especially from days 14‑28 post‑injury (P<0.001). The overall pathological score and degree of B1 methylation in the B1 siRNA‑treated group improved significantly at days 14‑28 post‑injury, with the maximum improvement observed on day 14 (P<0.01) compared with the NSS and Ca‑P nanoparticle groups. Immunohistochemical staining revealed lower expression of γH2AX and 8‑OHdG in the B1 siRNA‑treated group than in the control groups at days 14‑28 post‑injury; the maximum improvement was observed on days 14 and 21. These data imply that administering SINE siRNA is a promising therapeutic option for managing second‑degree burns.
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March-2022
Volume 16 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Meevassana J, Nacharoenkul P, Wititsuwannakul J, Kitkumthorn N, Hamill KJ, Angspatt A and Mutirangura A: B1 repetitive sequence methylation enhances wound healing of second‑degree burns in rats. Biomed Rep 16: 20, 2022
APA
Meevassana, J., Nacharoenkul, P., Wititsuwannakul, J., Kitkumthorn, N., Hamill, K.J., Angspatt, A., & Mutirangura, A. (2022). B1 repetitive sequence methylation enhances wound healing of second‑degree burns in rats. Biomedical Reports, 16, 20. https://doi.org/10.3892/br.2022.1503
MLA
Meevassana, J., Nacharoenkul, P., Wititsuwannakul, J., Kitkumthorn, N., Hamill, K. J., Angspatt, A., Mutirangura, A."B1 repetitive sequence methylation enhances wound healing of second‑degree burns in rats". Biomedical Reports 16.3 (2022): 20.
Chicago
Meevassana, J., Nacharoenkul, P., Wititsuwannakul, J., Kitkumthorn, N., Hamill, K. J., Angspatt, A., Mutirangura, A."B1 repetitive sequence methylation enhances wound healing of second‑degree burns in rats". Biomedical Reports 16, no. 3 (2022): 20. https://doi.org/10.3892/br.2022.1503