Profile of vitamin D receptor gene polymorphism TaqI in patients with periodontitis

Hamrun,Nurlindah; Ruslin,Muhammad; Marlina,Erni; Oktawati,Sri; Saito,Takashi; Yusuf,Andi Sitti Hajrah; Ou,Keng-Liang

doi:10.3892/br.2022.1518

Profile of vitamin D receptor gene polymorphism TaqI in patients with periodontitis

Authors:
- Nurlindah Hamrun
- Muhammad Ruslin
- Erni Marlina
- Sri Oktawati
- Takashi Saito
- Andi Sitti Hajrah Yusuf
- Keng-Liang Ou
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Affiliations: Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, South Sulawesi 90425, Indonesia, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hasanuddin University, Makassar, South Sulawesi 90425, Indonesia, Department of Oral Medicine, Faculty of Dentistry, Hasanuddin University, Makassar, South Sulawesi 90425, Indonesia, Department of Periodontology, Faculty of Dentistry, Hasanuddin University, Makassar, South Sulawesi 90425, Indonesia, Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 060‑8586, Japan, Department of Dentistry, Taipei Medical University‑Shuang Ho Hospital, New Taipei City 235, Taiwan, R.O.C.
Published online on: March 1, 2022 https://doi.org/10.3892/br.2022.1518
Article Number: 35
Copyright: © Hamrun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to assess the incidence of the vitamin D receptor (VDR) gene polymorphism TaqI in patients with periodontitis, and the potential association of this polymorphism with the severity of the disease. This was a case‑controlled study, which included 162 adults divided into two groups as follows: Case group (81 patients diagnosed with periodontitis) and control group (81 patients without periodontitis). Venous blood was obtained from each sample from which DNA was extracted. The gene polymorphism was determined using restricted fragment length polymorphism‑PCR and DNA sequencing to identify endonuclease restrictions in exon 9 (TaqI). The data were analyzed using an independent samples t‑test. VDR gene polymorphisms were detected in periodontitis cases with TT (86.4%), Tt (12.4%) and tt (1.2%) genotypes. DNA sequencing confirmed a change in the sequence of the VDR gene nucleotides in patients with periodontitis. The data indicated that the severity of periodontal tissue damage may be influenced by changes in the nucleotide sequence.

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