Evaluation of the presence of TRPC6 channels in human vessels: A pilot study using immunohistochemistry

Abdinghoff,Jan; Servello,Davide; Jacobs,Tobias; Beckmann,Anja; Tschernig,Thomas

doi:10.3892/br.2022.1525

Evaluation of the presence of TRPC6 channels in human vessels: A pilot study using immunohistochemistry

Authors:
- Jan Abdinghoff
- Davide Servello
- Tobias Jacobs
- Anja Beckmann
- Thomas Tschernig
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Affiliations: Institute of Anatomy and Cell Biology, Saarland University, Medical Campus, D‑66424 Homburg/Saar, Germany
Published online on: March 22, 2022 https://doi.org/10.3892/br.2022.1525
Article Number: 42
Copyright: © Abdinghoff et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The TRPC6 channel is permeable to calcium ions as well as other ions and plays an important role in the physiology and pathophysiology of vessels. Findings from animal and cell culture experiments have shown its involvement in important vascular processes such as the Bayliss effect or endothelial‑mediated vasodilatation. Furthermore, the relevance of TRPC6 channels in humans has become apparent based on diseases such as idiopathic pulmonary arterial hypertension, focal segmental glomerulosclerosis and atherosclerosis, amongst others. However, histological evidence that systematically detects TRPC6 channels in human vessels has not been provided to date. In this study, 40 vessel sections from nine body donors were obtained, processed and stained with a knockout‑validated antibody against the TRPC6 protein using immunohistochemistry and western blotting. More than half of the samples yielded evidence of TRPC6 channel expression in the intima and adventitia. TRPC6 channels were detected in the tunica media in only one of 40 cases. TRPC6 detection in the human intima confirmed several demonstrated physiological aspects of the TRPC6 channels in the vasculature and may also be involved in associated human diseases. The near absence of TRPC6 channels in the tunica media was in contrast to a view that is primarily based on animal studies, from which its presence was assumed.

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